Bacterial Toxin Research Citations

Enzyme-linked immunosorbent assay (ELISA):

Samples were grown overnight in LB-L media, then subcultured 1:100 in LB-L media and grown for 8 h at 37 C and 225 rpm. The cultures were pelleted by one centrifugation step of 2272g for 10 min and the supernatant was passed through a 0.45 m filter. The wells of a 96-well microtiter plate (Santa Cruz Biotechnology, Inc., part# sc-204463) was coated with 100 L of a solution of 4 g mL¹ protective antigen (PA) of the anthrax toxin (List Biological Laboratories, part# 171E) in 5 mM HEPES, 50 mM NaCl, pH 7.5 covered at 4 C overnight. Liquid was removed by inversion and wells were incubated with a 200 L blocking solution (200 L 2% milk, 0.05% TBST) at room temperature for 1 h. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Vaccines, TLR Agonists, and Multi-Analyte Assays:

Licensed vaccines were commercially obtained. Ultrapure LPS (Salmonella minnesota R595, List Biological Laboratories, Campbell, CA, USA) was used at 100ng/ml.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… PTX was obtained from List Biological Laboratories (Campbell, CA, USA) …

Coimmunoprecipitation:

Coimmunoprecipitation experiments were carried out as described before³⁵. Briefly, HEK293T cells were transiently transfected by using the calcium phosphate method. After incubation for 24h, the cells were stimulated with Ang II (1M) for the time indicated and harvested thereafter. For inhibitor experiments, cells were pretreated with the inhibitor or its control (Candesartan 100nM, PP2 10M, PTX 200ng/ml, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The following inhibitors were used: Pertussis toxin (PTX) (List Biological Laboratories, 100 ng/ml), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CNS Autoimmune Disease Induction and Analysis:

…Mice received 200 ng B. pertussis toxin (Ptx) (List Biological) by i.p. injection on days 0 and 2. …At day 0 and 2, mice received Ptx. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

…Normal mouse EC (NEC) were originally isolated from mouse mammary glands and cultured in EGM-2 [6]. Growth factors or lipopolysaccharide (LPS, List Biological Laboratories 201) were added to cultures at indicated concentrations.

• Product #201 – LPS from Escherichia coli O111:B4

Pertussis-Toxin List Biological Laboratories, Campbell,CA, USA

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peptide priming:

…On days 0 and 2, 300ng of pertussis toxin (Ptx, List Biological Labratories, Campbell CA, USA) was injected intraperionteallly in all immunizations to compare both the self and foreign immune responses.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Isolation and cultivation of OECs derived from luc tg rats:

…The composition of KCM is as follows: DMEM high-glucose medium (Gibco), Nutrient Mixture F-12 (Gibco), 10% fetal bovine serum (Japan Bio Serum), 400ng/mL hydrocortisone succinate (Wako), 2nM 3,3,5- triiodo-l-thyronine sodium salt (MP Biomedicals), 1nM cholera toxin (List Biological Laboratory), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and immunoblotting:

MCF10A and MCF10DCIS.com [37] cells were cultured in Hams F12/DMEM (Cellgro) supplemented with …100ng/ml cholera toxin (List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae