Citations

…8 BoNT/A-LC, BoNT/B-LC, and BoNT/C-LC were obtained from List Biological Labs, Inc. (CA, USA). …

• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant
• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant
• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

EAE:

… 200 ng of pertussis toxin (List Biological Lab, Epsom, England) was given intraperitoneally (ip) on days 0 and 2 post inoculation (pi). Mice were scored daily for clinical signs …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Each mouse was immunized with a subcutaneous injection of 20 l MOG 35-55 /CFA emulsion and an intraperitoneally injection of 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) in sterile PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

Ultrapure LPS (Escherichia coli 0111:B4) was obtained from List Biological Laboratories.

TLR4 is expressed on different subsets of immune and nonimmune cells [36], and the function of TLR4 varies by cell type. For example, we have shown that TLR4 is essential to LPS uptake by hepatocytes…

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Materials and Reagents:

Anthrax lethal toxins (PA and LF) and Escherichia coli O111:B4 LPS were purchased from the List Biological Laboratories.

Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Lethal Factor Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

RESULTS:

HDAC8 inhibition restores the production of pro-IL-1 in response to LPS in LeTx-exposed murine macrophages. LeTx suppresses expression of various inflammatory cytokines through inactivating MEKs (3-5). Consistent with these reports, a sub-lethal dose of LeTx (100 ng/ml PA & LF, a.a. for 4 h) completely cleaved MEK1 and inhibited expression of pro-IL-1 induced by LPS (100 ng/ml) for more than 2 days in RAW264.7 macrophages (Fig. 1A). …

Hormone treatment and induction of EAE:

Female C57BL/6 wild-type mice were implanted subcutaneously with 2.5mg/60-day release 17 -estradiol pellets … Mice received intraperitoneal injections of pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin and clodronate liposome injections:

Diphtheria toxin (lot no. 15043A1; List Biological Laboratories) was diluted in PBS and administered via intraperitoneal injection on days 2 (50 g/kg mouse) and 1 (10 g/kg) before intratracheal LPS, and on days +1, +3, and +5 (10 g/kg) following intratracheal LPS as previously described (52). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

Purified lipopolysaccharides (LPS) from Escherichia coli were obtained from List Biological Laboratory (Campbell, CA) and dissolved in endotoxin-free water. …

Immunoblot assay with hepatocytes and liver tissue:

For immunoblotting of caspase-1 and IL-1, hepatocytes were plated in 6-well plates (810⁵ cells/ml) and primed with LPS (500ng/ml) for 4hr. 

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Surgical procedures performed in mice: 

Tract tracing was performed by injection of Cholera toxin B (CTB) (List Biological Laboratory, Campbell, CA) 1 µl of 1% wt/vol in sterile water injected into both sciatic nerves three days before perfusion to visualize ascending sensory tract (AST) axons.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAE:

Mice (8-12 weeks old) were immunized subcutaneously in the hind limbs on day… Two hundred nanograms of pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)