Bacterial Toxin Research Citations

…,according to themanufacturer’s instructions as a positive control, the endothelial cells were incubated with LPS (1 ng/mL) from Escherichia coli 0111.B4 (List Biological Laboratories, Campbell, CA). …

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

… Mycobacterium tuberculosis H37Ra (Difco Laboratories). At the time of and 2 d after immunization, mice were injected ip with 300 ng Bordetella pertussis toxin (List Biological Laboratories) in PBS. Mice were examined daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Two hundred nanograms pertussis toxin (List Biological Laboratories, Campbell, CA) was administered iv on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…containing 400 µg killed Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI) on day 0 followed by ip treatments with 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… The animals also received 200 ng pertussis toxin (List Biological Laboratories) ip on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental animals:

… In a separate cohort of Gaa^/ animals (n = 4), intrapleural injection (20 l) of cholera toxin subunit dissolved in sterile saline for a 2% solution (List Biological Laboratories, Campbell, CA) was used to retrogradely label phrenic motoneurons. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE induction

EAE was induced in mice by subcutaneous (S.C.) injection into the hindquarters of 50 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅), …Immunized mice also received two intraperitoneal (IP) injections of pertussis toxin (Bordatella pertussis) (PT, List Biological LabsCampbell, CA, USA)first, on the day of the induction and again 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

CT was purchased from List Biological Laboratories (Campbell, CA). …

Analysis of lung migratory dendritic cells and BMDCs:

Mice (n = 15) were i.n. administered with 2 µg of CT and medLN cells were prepared before or 13 days after the administration. For i.n. administration, mice were lightly anesthetized by isoflurane (Ifran, Hana Pharm, Kyounggi-Do, Korea) inhalation and CT in a volume of 50 µl of phosphate-buffered saline (PBS) was applied to the left nostril. The CT-administered mice didnt have any pathologic appearance compared to untreated mice during the days. …

Cytokine assays:

BMDCs were incubated with PBS or CT (2 µg/ml) for the indicated days and the culture supernatants were harvested at the indicated time points. …

In vitro T cell differentiation:

To induce T cell differentiation in DC-assisted system, isolated in vivo migratory DC subsets, BMDCs (untreated or CT-treated), or phenotype-based, isolated BMDCs were used. …The BMDCs were incubated with CT for a day and in some experiments, CT-treated BMDCs were FACS sorted using FACSAria II (BD Bioscience) based on CD86 expression. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

Lipopolysaccharide (LPS) from Escherichia coli O111:B4 was purchased from List Biological Laboratories, Inc.

• Product #201 – LPS from Escherichia coli O111:B4

PBMC isolation and culture:

PBMC were isolated from collected blood or leukopheresis by density gradient centrifugation, according to the manufacturers instructions. Cells were cryopreserved in liquid nitrogen suspended in FBS containing 10% (vol/vol) DMSO. Culturing of PBMCs for in vitro expansion was performed by incubating in … and Fim2/3 (List Biological Labs) at 5 g/mL. …

• Product #186 – Fimbriae 2/3 from B. pertussis