Citations

Reagents:

Cholera enterotoxin was from List Biologicals (Campbell, CA, USA).

Tissue culture:

MCF-10A cells were maintained in DMEM/F12 (1:1) medium supplemented with 5% horse serum, 10 µg/ml insulin, 10 ng/ml EGF, 0.5 µg/ml hydrocortisone and 100 ng/ml Cholera toxin.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Lipid Analyses and Visualization:

T…  Cell surface GM1 levels were examined by flow cytometry. Cells were detached by trypsin and incubated with 1 µg/ml biotin-conjugated cholera toxin B subunit (biotin-CTxB) (List Biological Laboratories) for 60 min at 4°C. …

Immunofluorescence Microscopy:

Cells were seeded on glass coverslips in 6-well plates and grown for 2 days in medium A. After incubation of the cells in medium F overnight, they were fixed with 4% paraformaldehyde for 10 min and blocked with 5% FBS in PBS for 1 h. Specimens were incubated with biotin-conjugated CTxB (1 µg/ml) and anti-caveolin-1 antibody or with biotin-conjugated CTxB (1 µg/ml) and anti-flotillin-1 antibody for 1 h at room temperature. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

EAE:

To induce EAE, groups of 8-12 wk-old mice were immunized with 150 µg of MOG_35-55 emulsified 1:1 in CFA with 400 µg of Mycobacterium tuberculosis H37RA (Difco Laboratories) in the two flanks subcutaneously. 250 ng of pertussis toxin (List Biological Laboratories) was injected intraperitoneally on the day of immunization and 2 d later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde Labeling of VTA-Projecting Hypothalamic Neurons with CTB:

To label perikarya of hypothalamic neurons projecting to the VTA, the rats (n = 52) were given CTB (0.5% solution; #103, List Biological Laboratories, INC, Campbell, CA, USA) via unilateral iontophoresis (5 A, 7 s on-off) into the VTA for 20 min. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

CT-B processing:

Sections were rinsed (3 10 min) with 0.01 M potassium phosphate-buffered saline (KPBS, pH 7.4, 21C) and incubated overnight in 1antibody (goat anti-choleragenoid, List Biological Laboratories) at 4C.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… Pertussis toxin (PTX) was obtained from List Biological Laboratories (Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction by active immunization:

…They were also injected intraperitoneally with 20 µg/kg of PTX (List Biological Laboratories) immediately and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization with whole-cell vaccine:

Animals were intranasally immunized twice (with a week apart) with the whole-cell antigen (WCA) derived from strain RM200 (38). The dose of 10-l vaccine volume was made of 100 µg (dry weight) of WCA and 1 µg cholera toxin (CT) (List Biological Laboratories, Campbell, CA) as a mucosal adjuvant in PBS.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

7-week-old female C57BL/6 mice were purchased from DBL. EAE was induced by subcutaneous immunization with 100 µg of MOG₃₅₅₅ peptide…At 0h and 48h after immunization, mice were intraperitoneally treated with 200ng of pertussis toxin (List Biological Laboratories Inc.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS was from List Biological Laboratories Inc (Campbell, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/