Additional information
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Official purchase order required.
International shipping and handling: $50 fee added to each shipment.
Products are for research purposes only and are
not for use in humans or as diagnostic agents.
$700.00
#112 (0.2 mg)

Certificate Of Analysis
Form
Storage temp
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... along with biotin-conjugated Cholera toxin B subunit (1:500, List Labs, Cat# 112). ...
... Next, sections were incubated for 24 h at room temperature (RT) with a goat anti-CT-B antibody (List Biological Laboratories, CA; ...
Reagents and antibodies:
...Cholera toxin B subunit (CTxB) biotin conjugate was from List Biological Laboratories ...
FACS analysis:
Cell surface expression of gangliosides was analysed by flow cytometry. The following antibodies/agents were used to detect cell surface gangliosides; GM3, M2590; GM2, 1011; GM1, biotin-conjugated CTxB, ...
...Sections were then incubated with cholera toxin B subunit-biotin conjugate (List Biological Laboratories, Campbell, CA, USA) diluted at 0.5 µg/ml with PBS plus 3% BSA, at room temperature for 60 min. ...
Reagents and Cell Culture:
... The following probe/antibodies were used: biotin-conjugated cholera toxin B subunit (List Biological Laboratories, Campbell, CA, USA); ...
Immunofluorescent study:
Each section was pretreated in the same way as described above for immunohistochemistry. For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:500; List Biological Laboratories, Inc.) and ...
... We used Cholera toxin-B-biotin conjugate (List Biological Labs, Campbell, CA) as a probe for GM1 ...
Lipid Analyses and Visualization:
T... Cell surface GM1 levels were examined by flow cytometry. Cells were detached by trypsin and incubated with 1 µg/ml biotin-conjugated cholera toxin B subunit (biotin-CTxB) (List Biological Laboratories) for 60 min at 4°C. ...
Immunofluorescence Microscopy:
Cells were seeded on glass coverslips in 6-well plates and grown for 2 days in medium A. After incubation of the cells in medium F overnight, they were fixed with 4% paraformaldehyde for 10 min and blocked with 5% FBS in PBS for 1 h. Specimens were incubated with biotin-conjugated CTxB (1 µg/ml) and anti-caveolin-1 antibody or with biotin-conjugated CTxB (1 µg/ml) and anti-flotillin-1 antibody for 1 h at room temperature. ...
Antibodies and Regents:
...Cholera toxin B subunit-biotin conjugate reacting with GM1 is from List Biological Labs (Campbell, CA). ...
... Ganglioside GM1 (GM1) was detected using 1 µg/ml biotinylated cholera toxin B subunit (List Biological Laboratories Inc., Campbell, CA) ...
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List Labs
540 Division Street
Campbell, California
List Biotherapeutics
9800 Crosspoint BIvd Suite 2011
Indianapolis, IN 46256, USA





