Bacterial Toxin Research Citations

Immunizations, oral tolerance induction, and FTY720 treatment

To induce local IgA immunity mice were fed 10 mg of ovalbumin (OVA) protein grade V (Merck KGaA, Darmstadt, Germany) dissolved in 0.5 mL of phosphate buffer saline (PBS) containing 3% (w/v) sodium bicarbonate with or without the addition of 10 µg cholera toxin (CT) adjuvant (List Biological Laboratories, Campbell, CA, USA) to each dose. The mice were given three oral administrations, 10 days apart, using intragastric intubation. To induce oral tolerance, mice were fed three doses of 20 mg OVA dissolved in 0.5 mL of PBS containing 3% (w/v) sodium bicarbonate 7 days apart. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Brain Imaging

Mice were anaesthetized with isoflurane and rectal temperatures recorded before being inoculated i.t. with either EPS+, EPS- or 50 μg ultra-pure lipopolysaccharide from Escherichia coli O111:B4 (ListLabs; #421) in a final volume of 50 μl PBS. At 4 h post infection, the mice were anaesthetized under isoflurane and rectal temperatures recorded before intravenous perfusion with cold PBS. The mice were then perfused with cold 4% formalin. The brains were then harvested and stored in 4% formalin for 24 h at 4 °C. After 24 h, the brains were then washed with PBS 3 times (10 minutes each) while swirling. Brains were then stored in 20% sucrose and kept at 4 °C.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Phagocytosis Marker Assay

BMDMs were stimulated with LPS at 100 ng/mL (ULTRA PURE LPS from Escherichia coli O111:B4, #421, List Labs) or saline vehicle for 4 hours. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

2.2 DT or saline administration

Adult mice (6–16 weeks of age) received two intramuscular (IM) injections of DT (List Biological Laboratories, Campbell, CA) at either 25 ng/g (low-dose) or 50 ng/g (high-dose), spaced 2 days apart. Some control mice were administered IM saline.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The mice were also injected intravenously with pertussis toxin (1040ng; List Biological Laboratories) on the day of immunization and after 48h. A control (CTR) group of 19 mice received complete Freund’s adjuvant and pertussis toxin, but no MOG35-55 peptide. Four mice were used in preliminary MRI experiments to set up the acquisition protocol …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

nthesized by Shenggong Biotech Shanghai Co., Ltd. Complete Freund’s adjuvant (CFA)(SLBW1457) was purchased from Sigma?Aldrich (St. Louis, USA). Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA). HRP goat anti-rabbit antibody (GB23303) was obtained from Servicebio Biotechnology Co., Ltd. Anti-fluorescence quenching solution (P0126) and haematoxylin … nthesized by Shenggong Biotech Shanghai Co., Ltd. Complete Freund’s adjuvant (CFA)(SLBW1457) was purchased from Sigma?Aldrich (St. Louis, USA). Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA). HRP goat anti-rabbit antibody (GB23303) was obtained from Servicebio Biotechnology Co., Ltd. Anti-fluorescence quenching solution (P0126) and haematoxylin

Immunogenicity Studies of PA83 variants in Mice

Immunogenicity studies were performed with 3 and 10 μg of plant-produced dPA83(E), dPA83(P) and commercial recombinant PA (rPA, List Biological Laboratories, Campbell, CA, used as a positive control) proteins, administered intraperitoneally in seven- to eight-week-old Balb/c mice (6 animals/group). Mice were injected with three doses of protein samples adsorbed in 0.3% Alhydrogel at three-week intervals (day 0, 21 and 42). …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Goat anti-CTb (1:5000) List Biological Labs Cat# 703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.3 Antigen-induced EAE (aEAE) models

The aEAE models were established in our previous study.4, 5 Mice were anesthetized by intraperitoneal injection of pentobarbital (0.2 mL/kg) per our previous protocol. To actively induce disease, mice were subcutaneously injected with a 200-mL emulsion containing 200 µg of myelin oligodendrocyte glycoprotein peptides 35–55 (MOG35–55; amino acids 35–55 of bovine MOG; Sigma-Aldrich; purified using high-performance liquid chromatography; purity >95%) and complete Freund’s adjuvant Sigma-Aldrich, St. Louis, MO, USA) over six sites at the tail base and on the flank. At 0 and 24 h after immunization, mice were intraperitoneally injected with pertussis toxin (List Biological, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)