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December 9, 2023
Scientific Reports
Stern, D;Meyer, TC;Treindl, F;Mages, HW;Krüger, M;Skiba, M;Krüger, JP;Zobel, CM;Schreiner, M;Grossegesse, M;Rinner, T;Peine, C;Stoliaroff-Pépin, A;Harder, T;Hofmann, N;Michel, J;Nitsche, A;Stahlberg, S;Kneuer, A;Sandoni, A;Kubisch, U;Schlaud, M;Mankertz, A;Schwarz, T;Corman, VM;Müller, MA;Drosten, C;de la Rosa, K;Schaade, L;Dorner, MB;Dorner, BG;
Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
Recombinant antigens
Pertussis toxin (salt free) was obtained from List Labs (Campbell, CA, USA), …
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
December 9, 2023
Nature Communications
Elhai, M;Micheroli, R;Houtman, M;Mirrahimi, M;Moser, L;Pauli, C;Bürki, K;Laimbacher, A;Kania, G;Klein, K;Schätzle, P;Bertoncelj, MF;Edalat, SG;Keusch, L;Khmelevskaya, A;Toitou, M;Geiss, C;Rauer, T;Sakkou, M;Kollias, G;Armaka, M;Distler, O;Ospelt, C;
Product: LPS from Escherichia coli J5 (Rc)
SF stimulation
SF were stimulated with human recombinant TNF (10 ng/ml; R&D Systems), human recombinant IL1β (1 ng/ml; R&D Systems), lipopolysaccaride (LPS) from Escherichia coli J5 (100 ng/ml; List Biological Laboratories), polyI-C (PIC) (10 μg/ml; InvivoGen), bacterial lipopeptide (bLP) palmitoyl-3-cysteine-serine-lysine-4 (300 ng/ml; InvivoGen) or human recombinant TGFβ (10 ng/ml; R&D Systems) for 24 h. …
December 8, 2023
Research Square
Yamamura, T;Takewaki, D;Kiguchi, Y;Masuoka, H;Manu, M;Raveney, B;Narushima, S;Kurokawa, R;Ogata, Y;Kimura, Y;Sato, N;Ozawa, Y;Yagishita, S;Araki, T;Miyake, S;Sato, W;Suda, W;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
EAE induction
For EAE induction, mice were injected subcutaneously at two sites on the back with 200 mg MOG35–55peptide (synthesised by Toray Research Center, Tokyo, Japan) and 1 mg heat-killed Mycobacterium tuberculosis H37RA emulsified in complete Freund’s adjuvant (Difco, KS, USA). Four hundred nanograms of Pertussis toxin (List Biological Laboratories, CA, USA) were injected intraperitoneally on days 0 and 2 after immunisation. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
December 8, 2023
Science Advances
Zhai, D;Yan, S;Samsom, J;Wang, L;Su, P;Jiang, A;Zhang, H;Jia, Z;Wallach, I;Heifets, A;Zanato, C;Tseng, CC;Wong, AHC;Greig, IR;Liu, F;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Experimental autoimmune encephalitis
EAE was induced as described previously (33). Female C57BL/6 mice (9 weeks of age) were immunized by subcutaneous injection with recombinant MOG35–55 (200 μg, Biomatik Corporation, Cambridge, ON, Canada) emulsified in complete Freund’s adjuvant (incomplete Freund’s adjuvant, Sigma-Aldrich, Oakville, ON, Canada; 4 mg/ml Mycobacterium tuberculosis, strain H37Ra, BD Biosciences, Missisauga, ON, Canada) into four sites on the back (50 μl per site). Pertussis toxin (200 ng, List Biological Laboratories Inc., Campbell, CA, USA) was injected intraperitoneally on days 0 and 2 after immunization. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
December 7, 2023
Research Square
Wang, J;Lei, Y;Zhu, F;Yu, J;Huo, X;Yang, C;Zhao, P;Huang, Y;Hao, M;Liu, Y;Liu, X;Li, H;Sun, B;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.2. Induction of EAE
rMOG (2mg/ml) was emulsified with an equal volume of complete Freund’s adjuvant (CFA, 4mg/mL, Sigma, F5506). EAE animal model was induced by subcutaneous injection of the above emulsion into the back. 300ng pertussis toxin (List Biological Laboratories, 180228A1) was injected into the tail vein on the day of immunization and the second day after immunization. Female mice under the same conditions were used as control, and CFA was injected subcutaneously. …
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
December 7, 2023
Frontiers In Molecular Neuroscience
Quillet, R;Gutierrez-Mecinas, M;Polgár, E;Dickie, AC;Boyle, KA;Watanabe, M;Todd, AJ;
Product: Anti-Cholera Toxin B Subunit (Goat)
December 7, 2023
The Journal Of Neuroscience
Fisher, K;Garner, J;Darian-Smith, C;
Product: Anti-Cholera Toxin B Subunit (Goat)
… For CT-B processing, sections were blocked in 10% horse serum (2h, room temperature) and incubated in anti-CT-B made in goat (List Biological #703; 1:4000 dilution) in TBS-TX/3% horse 234 serum for 2-3 days at 4oC. After thorough washing, sections were further incubated in ImmPRES …
December 5, 2023
Cell Metabolism
De Virgiliis, F;Mueller, F;Palmisano, I;Chadwick, JS;Luengo-Gutierrez, L;Giarrizzo, A;Yan, Y;Danzi, MC;Picon-Muñoz, C;Zhou, L;Kong, G;Serger, E;Hutson, TH;Maldonado-Lasuncion, I;Song, Y;Scheiermann, C;Brancaccio, M;Di Giovanni, S;
Product: Anti-Cholera Toxin B Subunit (Goat)
… Anti-Cholera Toxin B Subunit (Goat) List Biological Laboratories Cat#703; …
December 4, 2023
International Journal Of Molecular Sciences
Holloman, JP;Dimas, SH;Archambault, AS;Filipello, F;Du, L;Feng, J;Zhao, Y;Bollman, B;Piccio, L;Steelman, AJ;Hu, H;Wu, GF;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
4.4. EAE Induction and Clinical Score Assessment
Active EAE was induced as previously described [34]. Briefly, mice were subcutaneously injected with 200 μg of MOG35–55 peptide (Genscript, RP10245) emulsified in incomplete Freud’s adjuvant (Sigma, F5506; St. Louis, MO, USA) with mycobacterium tuberculosis H37Ra (BD Difco, BD 231141; Pittsburgh, PA, USA) and intraperitoneally injected with 200 ng of Pertussis Toxin (List Biological Laboratories, Campbell, CA, USA). …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
December 1, 2023
Thesis
María Josefina Peña Mosca
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Materials
Pertussis toxin from Bordetella pertussis 180 List Biological laboratories
Pertussis toxin mutant from Bordetella pertussis 184 List Biological laboratories
3.2.3. Inhibition of Gi-coupled receptors
3.2.3.1. In-vitro inhibition
Cells were cultured as described in 3.2.2.1 with 200 ng/ml pertussis toxin (PTx) for 2 h. Control cells were incubated with a PTx inactive mutant in similar conditions.
3.2.3.2 In-vivo inhibition
To inhibit chemokine-coupled receptors, mice were i.p injected with 12,5 µg PTx. Control mice were injected with a PTx inactive mutant in similar conditions.
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #184 – Pertussis Toxin Mutant
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