Bacterial Toxin Research Citations

… We used cholera toxin B subunit (CTb) as a retrograde tracer to identify neurons in the vBNST with direct efferent projections to the VTA. Rats were anesthetized with ketamine and xylazine and placed in a stereotaxic frame. Heat-sterilized Hamilton syringes were used to pressure inject 0.2 µl biotinylated CTb (List Biological Laboratories) unilaterally into the VTA over a 10 min period (posterior to bregma 5.6 mm, lateral 2.6 mm, ventral 8.6 mm; Paxinos and Watson, 2006). Syringes were left in place for 20 min after injections and slowly removed from the brain over a 10 min period. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… In vivo transport assays were performed in 6 months old WT (n=4) and -adducin KO (n=4) mice. Mice were anesthetized with ketamine (75 mg/kg) and medetomidine (1 mg/kg) and analgesia with buprenorphine (0.08 mg/kg) was done prior to the cirurgical procedure. Eyes were wet with local optic anesthetic (1 drop per eye). Injection was performed intravitreously in a 45°C angle with a 30-33 gauge needle syringe with 2 µl of the 0.1% cholera toxin subunit conjugated with Alexa Fluor 568 (List Biological Laboratories) with a rate of 1 µl per 15 seconds, at most. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cholera toxin B (CTB, 1%, 200 nl, List Biologicals) was bilaterally microinjected at T2 centered on the intermediolateral cell column, two injections each of 200 nl per side and the wound closed. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Dorsal column injury:

Three days before perfusion, animals (Fisher rats) in the 8-week time point after dorsal root injury and in the dorsal column injury group received 3 µl of 1% cholera toxin B (CTB) (103B, List Laboratories, Inc., Campbell, CA) into the sciatic nerve to transganglionically label dorsal root and dorsal column axons.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Spinal Cord Injections:

Layer V corticospinal motor neurons were labeled by stereotaxic injection of cholera toxin B subunit (CTB), a retrograde neuronal tracer, into the cervical enlargement at C5 of 5 month-old CD1 mice….and 500 nL of CTB tracer (6.67 g per l, List Biological Labs) was injected into the right dorsal corticospinal tract (dCST)⁵⁴. CTB infusion progressed at 0.2 mL per min through a glass micropipette connected by special adapters and high pressure tubing to a 10 mL Hamilton syringe….Cervical cord was cryosectioned at 40m and for dCST injection site verification by CTB immunofluorescence (Goat anti-CTB, List Biological Labs Product #703).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE Induction and Treatment:

Mice were immunized s.c. for active induction of EAE as described… Two hundred nanograms of pertussis toxin (List Biological Lab, …were given intraperitoneally on days 0 and 2 post-immunization (p.i.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Western blot analysis was performed as in [24] with the following modifications. Primary antibodies were diluted in blocking buffer at 1:1000 (Goat anti-PA polyclonal, List Biological Laboratories) or…

Reagents:

Anthrax Lethal Factor and Protective Antigen were purchased from List Biological Laboratories, Inc.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, Calif., USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every 2 days to induce chronic endotoxemia (n = 9 for each designated time point). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Murine EAE model:

EAE was induced in female WT and Abcc8/ mice at 8 weeks of age, …Each mouse then received 400 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (IP) on post-induction day (pid) 0 and pid-2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)