Bacterial Toxin Research Citations

Characterisation of the model:

Podo-DTR mice and wild-type littermates as controls were injected with a single dose of diphtheria toxin (DT) (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA) ranging from 0.1166ng/g body weight (bw). …

ACEi protection experiment:

Groups of 16 transgenic (Tg) mice and eight wild-type (WT) littermates were given captopril (200mg/L) (Product no. C4042, Sigma Aldrich, UK) in their drinking water or placebo (water alone) 24h after a single intraperitoneal (i.p.) injection of diphtheria toxin at 5ng/g bw (Product no. 150, Lot 15023A1; List Biological Laboratories Inc., California, USA). …

Urine and blood analysis:

Urine and serum creatinine concentrations were measured using the creatinase reaction, with the exception of the first cohort of the model evaluation studies where Jaffe reaction was used (for the Podo-DTR line 47 given 1ng/g DT).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Intracellular Cytokine Staining:

PBMCs collected from chronically rhCMV-infected rhesus monkeys stimulated with 10 g/mL Staphylococcus aureus enterotoxin type B (List Biological Laboratories) were included in each experiment as a positive control.

Experimental autoimmune encephalomyelitis:

EAE was induced in 8-10 week old C57BL/6 female mice using a previously established protocol by Miller… Pertussis toxin (200 ng) (List Biological Laboratories Inc., Campbell, CA) was injected intraperitoneally on the day of and 48 h following immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study Groups:

We studied two groups of 6 supine mechanically ventilated sheep. …During this ventilation period, sheep received a continuous intravenous infusion of endotoxin (10 ngkg^-1min^-1, Escherichia coli O55:B5, List Biological Laboratories Inc, California).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

When cells were treated with levosimendan or pimobendan, these agents were added 30 minutes before the challenge with lipopolysaccharide (LPS; Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Cell culture and transfection:

The breast cancer cell line MCF-7 and the normal breast cell line MCF-10A, …and cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA). MCF-7 was grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS). All cells were cultured on the surface of a 75-cm2 culture flask. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Eight-ten week old male wild-type (WT) C57BL/6JBomTac (Taconic, Skensved, Denmark) and syndecan-4 knock-out (KO) mice [20] were randomized to intraperitoneal injection with a single dose of LPS (10 mg/kg, List Biological Laboratories Inc., Campbell, CA) dissolved in …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Animals and EAE induction:

Female C57BL/6 mice averaging 8 weeks of age were obtained from Dooyeol Biotech (Seoul, Korea). …EAE was induced by subcutaneous immunization in C57BL/6 mice with 50 µg MOG₃₅₅₅ (peptide MOG; …In addition, 300 ng pertussis toxin (List Biological Laboratories, CA, USA) was administered by intravenous injection on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Un-nicked Diphtheria Toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA) and was used to deplete Foxp3+ Tregs from DEREG mice (Lahl et al., 2007) by administering 1g i.p. DT per 2 days from day -2 to day 6 p.i. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Tracer injections:

Rats were given intraperitoneal injections of a mixture of 45% ketamine (100 mg/ml), 35% xylazine (20 mg/ml) and 20% saline at a dose of 0.16 ml/100 g body weight, and several minutes later were placed into a Kopf stereotaxic instrument. Small bore holes were drilled in the skull above targeted structures and 1% Fluorogold (FG, Fluorochrome, Inc, Englewood, CO) in 0.067 cacodylate buffer or 10% subunit of cholera toxin (Ct; List Biological Laboratories Inc., Campbell, CA) in 0.1M Sorenson’s phosphate buffer (SPB; pH 7.4) were injected iontophoretically into the brains from 1.0 mm filament-containing borosilicate glass pipettes pulled to outside diameters of 15-18 m (for FG) or 20-25 m (for Ct). The injections were made over a period of 15 min using positive pulses (7s on/7s off) of 1 A (for FG) or 3 A (for Ct). Animals were given subcutaneous (s.c.) injections of carprofen (20 mg/kg) immediately after surgery and once daily for the next 3 days. Stereotaxic coordinates were established with the aid of the atlas of Paxinos and Watson (1997). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt