Citations

Animals and EAE induction:

C57BL/6J, 2D2 and Rag1^-/- mice were obtained from the Jackson Laboratories (Bar Harbor ME). IFNAR1^-/- mice (Ifnar1) were obtained from the Mutant Mouse Regional Resource Centers (MMRRC). 2D2 mice [25] were maintained under our established breeding protocol at the CHU de QubecUniversit Laval. Six (6) to 10 week old female mice were used in all experiments. For in vivo T cell transfer, stimulated cells were injected in cold PBS (Cellgro) intravenously, 5 x10⁶ cells per Rag1^-/- recipient. Recipient animals received 200 ng of pertussis toxin (List Biological Labs) intraperitoneally on day 0 (d0) and d2 post-transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Botulinum Neurotoxin Type B Light Chain (BoNTA-LC) was purchased from List Biological Laboratories (CA, USA).

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

Materials and methods:  Puried CT was purchased from List Biological Laboratories. …

CT used to perform the following (refer to paper for details): 

Cell stimulation, cAMP assay and flow cytometry.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experiments were performed on adult male C57BL/6 mice …The mice were anesthetized with isoflurane (2 %) and 9-15 nanoliters of a 0.2% solution of cholera toxin b subunit (CTb; List Biol. Lab) were injected into the intended target following craniotomy or laminectomy as described previously (Lu et al., 2000; VanderHorst and Ulfhake, 2006). …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Histology- antibody characterization:

Antibodies used in this study (see Table 1) were as characterized as follows. …The staining patterns with the CTb antibody depended solely upon the site of CTb injection; this antibody stains nothing in animals not injected with CTb.

Table 1…CTb, Cholera toxin b subunit, List Biological, goat polyclonal, 703, AB_2341545, 1:10,000. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Key reagents and their sources were as follows: Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories), …

• Product #201 – LPS from Escherichia coli O111:B4

Stereotaxic injections of tracers and viruses:

For peripheral sensory nerve labeling experiments, ~ 1.0 2.0 L of 1 % (w/v) cholera toxin subunit B (CTb, … List Laboratories) (Angelucci et al 1996) was injected subcutaneously and unilaterally on the left hemi-section of the face into one of maxillary, mandibular, or ophthalmic nerve terminal branching zones of trigeminal ganglion (Vg) afferent neurons. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of experimental autoimmune uveitis:

… The reactivity of the immune system was enhanced by intraperitoneal application of 1.2 µg pertussis toxin (List Biologicals, Campbell, CA) dissolved in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell isolation and propagation:

Adjacent normal lung tissue from a surgical specimen of lung tumor was obtained with the written informed consent of the patient. …1 nM cholera toxin (List Biological Labs), …All cells were maintained at 37°C in a humidified incubator, with 5% CO2. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

siRNA, antibodies and reagents:

Pertussis toxin (PTX)…was purchased from List Biological Laboratories.

Active systemic anaphylaxis:

Active systemic anaphylaxis model was achieved as previously described²⁰: five mice were immunized with i.p. injection of 1mg BSA and 300ng pertussis toxin as adjuvant in normal saline.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Culture of porcine urothelial cells:

Porcine urothelial cells (PUC) were isolated either from bladder biopsies or ureter specimens of minipigs under sterile conditions. … Isolated cells were washed in keratinocyte serum-free medium (KSFM; Invitrogen), supplemented with 50 µg/ml bovine pituitary extract, 5 ng/ ml human recombinant epidermal growth factor (both Invitrogen), 30 ng/ml cholera toxin (List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae