Citations

Cell culture:

For primary adult microglia culture CNS mononuclear cells were isolated from brain and spinal cord of 46 week old mice under sterile conditions and cultured in VLE-DMEM (Biochrom) with 10% FCS, 50 M -ME and 15% of M-CSF containing supernatant from L929 cells. The protocol was adapted from Ponomarev [21]. Cells were stimulated on d14 with 50 g/ml poly (I:C), 6 g/ml CpG2216 (TIB MOLBIOL), 100 ng/ml Lipopolysaccharide (LPS) from Salmonella minnesota R595 (List Biological Laboratories, Inc.), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Mice:

For Cre activation, tamoxifen…was given via gavage to neonatal mice (0.075mgg¹ for Lgr5EGFP-CreERT2 and 0.75mgg¹ Plp1-CreERT strains) and dams (0.225mgg¹, i.p.). Diphtheria toxin (6.25ngg¹ i.p., List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

To identify the retinofugal projection, 4 days before sacrifice, 2.5 m lof the orthogradely transported tracer CTB was intravitreally injected (1%, diluted in distilled water; List Biological Laboratories, Campbell, CA) following previously described protocols that are standard in our laboratory …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Female BALB/c mice (n = 10) were sensitized to EW by oral gavage (5 mg of protein plus 10 µg of cholera toxin List Biologicals, Campbell, CA per mouse), as described by Jimnez-Saiz, Rupa, and Mine (2011). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Intraperitoneal (ip) injections of pertussis toxin (200 ng) in phosphate buffered saline (List Biological Laboratories, Campbell, CA) were given on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Culture: Human Oral Mucosal Epithelial Cell Sheets:

…1 nM cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Lipopolysaccaride (LPS) from Salmonella typhimurium was from List Biological Laboratories (Campbell, CA). …

• Product #225 – LPS from Salmonella typhimurium

Induction of chronic relapsing EAE and neurological assessment:

Female C57BL/6 mice (8-12 weeks of age) were purchased from Charles River Laboratories. Mice were injected subcutaneously (s.c.) in the back with 200µL of recombinant MOG_35-55 (200µg, Biomatik Corporation, Cambridge, ON, Canada), which was emulsified in incomplete Freund’s adjuvant (Sigma, Oakville, ON, Canada) and supplemented with 4 mg/mL Mycobacterium tuberculosis (strain H37Ra, BD Biosciences, Missisauga, ON, Canada) in four different sites (50µL per site). Two hundred nanograms of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) was injected intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mycobacterium tuberculosis (H37Ra strain; BD Diagnostics). On the day of immunization and 48 h later, the mice were also injected iv with pertussis toxin (200 ng/mouse; List Biological Laboratories) in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction in mice:

…A total volume of 100 µl was injected subcutaneously (s.c.) on each side of the tail base. The mice were also given 200ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitonally (i.p.). Another 200ng of pertussis toxin was given 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)