Citations

… goat anti-CTB antibody (List Biological Laboratories, Campbell, CA, USA), …were used as primary antibodies. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Cell Preparation, Reagents, and Stimulation:

In some experiments, cells were pretreated with Ac-YVAD-cmk (50 M, ALX-260028-M001, Enzo Life Sciences) for 30 min before S. Typhimurium infection or fliC transfection. Otherwise, cells were treated with anthrax protective antigen (1 g/mL, List Biologicals)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunofluorescence microscopy:

… To detect the localization of GM1 gangliosides, TG cell monolayers were incubated for 5min with biotin-cholera toxin B subunit (CTB) (10µg/ml, List Biological Laboratories, Campbell, CA), rinsed thrice in RPMI 1640 medium, and then incubated with L. monocytogenes for 30min at 37°C. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… For the rPA, 7.5 g of rPA (LIST Biological Laboratories) was mixed with alum and either saline or 50 g of Poly I:C was added to the mix on the day of immunization. Antibody assays. To measure levels of antibodies to the rPA …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Competition binding assay:

Apparent dissociation constants (K_d values) for the binding of actoxumab and bezlotoxumab to various purified toxins were determined by competition ELISA, as described previously (14). Briefly, high-protein-binding ELISA plates (Fisher Scientific, Waltham, MA) were coated overnight at 4C with 0.5 g/ml TcdA or TcdB (List Biological Laboratories, Campbell, CA), washed, and subsequently blocked for 1 h at 37C.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Toxoid vaccine preparation:

Vaccine for immunization of animals was prepared in the following way: lyophilized C. difficile toxins A (Product #152C) and B (Product #155) (List Biological Laboratories, Campbell, CA, USA) were separately reconstituted and then dialyzed into 20mM MOPS-HCl pH 7.4, 150mM NaCl, at concentrations of ~0.6mg toxin per ml.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

…mixed with 10 µg of cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA) once a week for three consecutive weeks, or …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents and antibodies:

Cholera toxin (Product #100)…were from List Biological (Campbell, CA, USA)…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and methods

Cell culture:

…100 ng/mL cholera toxin (List Biological Laboratories),…

Three-dimensional morphogenesis assay:

Forty microliters of Matrigel (growth factor reduced, BD) was added to each well of 48-well plates and then the plates were incubated at 37­°C to allow solidification of the Matrigel. MCF10A cells were trypsinized and resuspended in assay medium (DMEM/F12 supplemented with…100 ng/mL cholera toxin, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS from Escherichia coli K12 D31m4 (Re) was obtained from List Biological Laboratories (Campbell, California, USA).

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)