Bacterial Toxin Research Citations

Retrograde labeling of spinal motoneurons and dorsal root ganglion neurons:

To label spinal motoneurons and dorsal root ganglion neurons, the tracer was then placed at the cut end of sciatic nerve 1cm distal to the anastomosis in the polyethylene capsule containing a solution of 1% cholera toxin subunit B low salt (CTB; List Biological Laboratories, Campbell, CA, USA) and left in place for 30 minutes. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Macrophage isolation and cell culture:

Collected bone marrow was grown on nontissue culturetreated dishes for 6 days, then plated on tissue culture dishes,…and/or LPS (10 ng/ml; Salmonella minnesota; List Biologicals) such that BMMs were harvested on day 7.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Experimental Autoimmune Encephalomyelitis:

… On days 0 and 2, mice received 200 µg of pertussis toxin (List Biological) intraperitoneally. Development of disease was monitored daily according to the following criteria: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell treatment and cytokine measurements:

…Treatments included each fraction or starting material alone or in combination with 10 or 100 ng/mL of LPS from Salmonella minnesota R595 (List Biological Laboratories, USA) for 1618 hr. Supernatants were collected, centrifuged at 16,000 x g for 5 min. and stored at -80C until analysis…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Briefly, 3.75 mg (1 mg protein) of Kraft All Natural peanut butter was gavaged with 5 µg or 2.5 µg of cholera toxin (CT) (List Biological Laboratories, Campbell, CA, USA) once a week for 4 weeks or for 10 consecutive days, respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Isolated macrophage cultures (n = 4) were treated with …ultrapure E. coli LPS (O111:B4; List Biological Lab), …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Quantum Dots:

As a positive agonist of pro-inflammatory responses, cells were treated with 10 ng/ml of E. coli LPS (List Biologicals, Campbell, CA) for 24 h for MTEC and 4 h for macrophages, in volumes of medium equivalent to those used for Qdots.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cells, mice and reagents:

… LPS (Escherichia coli 0111:B4) from List Biological Laboratories; …

Results

Dex enhances LPS-induced expression of Lcn2 and PTX3 in macrophages:

Consistent with previous observations (30, 31), Dex suppressed expression of the NF-B-regulated cytokine genes Il6 (encoding IL-6) and Il12b (encoding IL-12 p40) in LPS-stimulated BMMs at both mRNA (Fig. 1A) and protein levels (Fig. 1B). Although LPS elicits expression of Lcn2 and Ptx3 via NF-B activation in macrophages (11, 13, 18, 32, 33), little is known whether Dex affects LPS-induced expression of Lcn2 and Ptx3. …

• Product #201 – LPS from Escherichia coli O111:B4

B cell enrichment and proliferation analysis:

B cells were enriched by negative selection using biotin-conjugated anti-CD43 (S7) antibody B cells (2 10⁵/well) were plated in 96-well flat-bottom plates and stimulated with LPS (10 g/ml) (List Biological Laboratories, Campbell, CA),

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

…Escherichia coli 0111:B4 LPS

PMN and macrophage harvests and culture:

Macrophages were stimulated with media,…LPS (List Biologicals, Campbell, CA; 10ngml¹),…for 24h and cell supernatants harvested for cytokine analysis.

• Product #201 – LPS from Escherichia coli O111:B4