Bacterial Toxin Research Citations

Preparation of Mice:

…Diphtheria toxin (List Biologicals, Campbell, CA) was administered intraperitoneally as described in the online supplement. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Antibodies and regents:

… Cholera toxin B (CTx-B) subunit-biotin conjugate reacting with GM1 is from List Biological Labs (Campbell, CA). …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

All animals used were pathogen-free adult male Sprague-Dawley rats (300-350g)

Animal lesion surgery:

…Injections of ibotenic acid (n = 11, IBO, Tocris, Ellisville, MO), 0.9% saline (n = 26, Med-Vet, Mettawa, IL) or cholera toxin subunit B (n = 4, CTB, List Biological, Campbell, CA) were administered directly into the brain using a fine glass pipette (1 mm glass stock, tapering slowly to a 10-20um tip) connected to an air compression system.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials and Methods:

… Pertussis toxin (PTX) was from List Biologicals (Campbell, USA) (stock solution: 0.5 µg/µL in water), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were injected in both thigh muscles with 50 L of a formulation containing…or 20 g/mL anthrax recombinant protective antigen (rPA) (List Biological Laboratories, Campbell, CA)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Lung culture, drug treatments and imaging for morphometry:

… Lung cultures were treated with the following additions to the media to achieve final concentrations as follows: …botulinum toxin, serotype A (BTx): 50, 100, or 200 ng/mL (List Biologicals #128A, Campbell, California, USA). BTx was stored at 4°C (in a locked refrigerator), reconstituted at 100 ng/µL in water with 1 mg/mL BSA and handled in a fume hood. …

Neuronal tracing of afferent projections from C2 nerve:

Adult male Wistar rats (250 g, n = 5) were anaesthetised with 5 % isoflurane in O₂. A dorsal midline incision was then made from the base of the skull to the scapula. …Tissue was then sectioned coronally at 50 m and washed three times in PBS before incubation in goat anti CTb (1:10,000 in PBS containing 0.1 % triton X-100, List Biological) overnight at 4 C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction:

… In addition, the mice received 400 ng pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and induction of EAE:

… A second injection of MOG emulsion was injected in the left flank one week later. In addition, animals received an intraperitoneal injection of 500 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in 0.2 mL PBS on days 0 and 2 of the study. Normal control mice were anesthetized and received sterile PBS injections of equal volume in place of MOG and pertussis immunizations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Generation and polarization of macrophages:

…M0 macrophages were also similarly generated from fresh monocytes isolated from PBMCs by the Percoll-based method. For M1 or M2 macrophage polarization, M0 cells were treated for 2 days with 20 ng ml 1 recombinant human IFN g (Life Technologies) and 100 ng ml 1 Escherichia coli LPS (List Biological Laboratories, Campbell, CA), or 20 ng ml 1 recombinant human interleukin 4 (Life Technologies), respectively.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/