Bacterial Toxin Research Citations

 In vitro Lipopolysaccharide and Q-VD-OPh Stimulation:

After 24 h, the cell culture medium was replaced with DMEM/ antibiotics. WT and caspase-6 KO primary microglial cells were stimulated with 10 ng/ml (diluted in sterile water) of Escherichia coli lipopolysaccharide (LPS; 055:B5; List Biological Laboratories, USA) or sterile water (control) each 12-well plate always contained both treatments. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… Goat anti cholera toxin B-subunit (anti-CTb), List Biological Laboratories, Inc. … Cholera toxin subunit B [CTbList Biological Laboratories, Campbell, CA, USA] diluted in distilled water [1%] was used for the retrograde tracer injection control experiments. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Isolation and culture of human urothelial cells

… Isolated cells were washed in keratinocyte serum-free medium (KSFM; Invitrogen) supplemented with 50 µg/ml bovine pituitary extract, 5 ng/ml human recombinant epidermal growth factor (both Invitrogen), and 30 ng/ml cholera toxin (List Biological Laboratories). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE and Evaluation of Clinical Disease:

EAE was induced in mice as previously described… Subsequent to immunization, the mice received intraperitoneal injections of pertussis toxin (500 ng; List Biological Laboratories, Campbell, CA, USA) on the first day of sensitization and again after 2 d.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Recombinant protective antigen of anthrax (PA) and recombinant lethal factor (LF) were purchased from List Biologicals (Campbell, CA, USA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction of EAE (in mice): 

EAE was induced by subcutaneous injection on both flanks and base of the tail of 200 µg myelin oligoglycoprotein (MOG_35-55) peptide (Alpha Diagnostic Intl. Inc.) emulsified in 200 µl of complete Freund’s adjuvant (Difco). 200 ng of Bordetella pertussis toxin (List Biological Laboratories, Inc.) was injected intraperitoneally in 200 µl on the day of immunization and 48 hours later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization screening:

The threshold dose of PA for rabbits was chosen by injecting rabbits intramuscularly (i.m.) on days 0 and 14 with either 10 g, 1 g, 0.1 g, 0.01 g, or 0 g of PA (List Biological Laboratories, Campbell, Calif.) diluted in PBS in a volume of 0.5 ml, each adsorbed to 1 mg of aluminum per ml … The primary and secondary antibody response profiles were examined by injecting rabbits i.m. with 50 g of PA/Alhydrogel on days 0 and 21 and collecting blood periodically to test serum for the presence of antibodies to PA and TNA titers in the serum.

2.3. Protection against aerosol exposure

Rabbits were injected i.m. with 0.1 g of PA/Alhydrogel and boosted with 0.1 g of PA/Alhydrogel 4 days (day 86), 8 days (day 82), or 12 days (day 78) before aerosol exposure on day 90 following the primary immunization. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE induction:

Male C57BL/6 mice (810 wk old) were induced with EAE using 50 mg MOGp and 125 mg desiccated Mycobacterium tuberculosis H37Ra …as well as i.p. injections of 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) in PBS on days 0 and 2, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… As controls of cell responsiveness, cells were exposed to 10 ng/ml Salmonella minnesota ultrapure lipopolysaccharide (LPS; List Biologicals Laboratories, Campbell, CA), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE:

… MOG 35-55 peptide emulsified in 100 µl of IFA (Thermo Scientific) and PBS (1:1) supplemented with 250 µg Mycobacterium Tuberculosis H37RA (Difco Laboratories Inc.) on day 0. They were intraperitoneally administrated with 200 µg of pertussis toxin (List Biological Labs, Inc …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)