Bacterial Toxin Research Citations

EAE was induced using standard protocols as described … Pertussis toxin (PT) (300 ng) (List Biological laboratories Inc., Campbell CA, USA) was injected intraperitoneally (pH=7, in saline) on day 0 and day 2. MOG71-90 and MOG101-120 were not sufficient to induce EAE in any genotype. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Reagents:

… The cross-reactive material (CRM197), which is a nontoxic mutant of diphtheria toxin, was purchased from List Biologicals Laboratories, Inc. (Campbell, CA).

Conjugation of 1 with TT and CRM₁₉₇ carrier proteins:

The thiolated 1 was conjugated to the TT and CRM₁₉₇ carrier proteins in a two-step reaction using the NHS-(PEG)₂-maleimide crosslinker [(SM-(PEG)₂]. …

Conjugates with varying hapten densities were obtained by treating the TT and CRM₁₉₇ carrier proteins with an increasing molar conjugation ratio of the (SM-(PEG)₂ crosslinker to the carrier protein. The TT and CRM₁₉₇ carrier proteins were dialyzed overnight against PBS, pH 7.2. Aliquots containing 2 mg of the protein were mixed with increasing excess molar ratios (5, 25, 100, 200, 400 and 800) of the SM-(PEG)₂ crosslinker (250 mM stock in DMSO) and incubated for 2 h at RT. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

Induction of EAE:

EAE was induced using standard protocols as previously described [24, 34, 35]. Briefly, 810 week old female WT and cathepsin B, S, L or B and S deficient mice were anesthetised with ketamine-xylazine. These mice were subsequently injected subcutaneously with 200 µl of an emulsion of 50 µg MOG35-55 in complete Freunds adjuvant (0.5 mg/ml M. butyricum in paraffin oil) (CFA) (BD Difco, Franklin Lakes NJ, USA). Pertussis toxin (PT) (300 ng) (List Biological laboratories Inc., Campbell CA, USA) was injected intraperitoneally (I.P.) (pH = 7, in saline) on days 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Culture media and supplements:

… The culture medium was supplemented with 250 ng/ml amphotericin B solution (HyClone), 20 ng/ml cholera toxin (List Biological),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Methods:

… Adult male SpragueDawley rats (290360 g; Harlan) under anesthesia (1 ml/kg body weight of 50 mg/ml ketamine and 10 mg/ml xylazine, intramuscular) received single, iontophoretic injections of a mixture of 2.5% P. vulgaris leucoagglutinin (PHAL; Vector Labs) and 0.25% cholera toxin B subunit (CTB; List Labs) targeted stereotaxically to the LHAjv region (Swanson, 2004). … For IHC detection of tracers, the sections were incubated in primary antibodies directed against either CTB (1:10,000; goat, List BioLabs) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… An incision was made in the neck, and the Xth cranial nerve was isolated from the carotid artery and injected 2 cm distal from the head one to two times with the neuronal tract tracer cholera toxin B (CTB, 1% in dH 2 O, … List Biological Laboratories, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of diarrhea:

Cholera diarrhea was induced by oral administration of 25 µg cholera toxin (List Biological Laboratories, Campbell, CA)⁴⁴. Fifteen hours later, we examined the water volume in the intestinal lumen.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and Evaluation of EAU:

Lrrk2 (-/-) mice and their WT controls were immunized with bovine IRBP (150 g) and human IRBP peptide 120 (200 µg), emulsified with complete Freunds adjuvant (CFA) containing 2.5 mg/ml killed Mycobacterium tuberculosis (DIFCO, Detroit, MI), administered subcutaneously in a volume of 0.2 ml. In addition, the mice were injected intraperitoneally with 0.2 µg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS derived from Salmonella typhimurium from List Biological Laboratory (Campbell …

Placental macrophage and THP-1 cell treatment:

… For COX-2 selective inhibition experiments, cells were pretreated with 1 M NS-398 for 30 min, and then treated for 24 h with solvent control or 180 M MEHP with or without 1 M NS-398. Effective inhibitor concentrations were identified based on preliminary concentration response curves in THP-1 cells, using 500 ng/mL LPS as an inducer of COX-2 (data not shown). …

• Product #225 – LPS from Salmonella typhimurium

Immunohistochemistry:

After post-operative survival times of three and five months, mice were terminally anaesthetized and perfused with room temperature saline, followed by a fixative solution containing cooled 4% w/v formaldehyde and 14% v/v saturated picric acid in PBS (pH 7.35 7.45). … The anti-cholera toxin subunit B (CTB, List biological laboratories) antibody was incubated for 4h at room temperature and…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)