Bacterial Toxin Research Citations

EAE Induction:

EAE was induced by immunization…followed by two doses of 150 ng pertussis toxin (List Biological Laboratories) i.p. on day 2 and day 4 post-immunization. Mice were monitored for signs of disease as previously described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and cell transplantation:

… The mice also received 350 ng pertussis toxin (List Biological Laboratories Inc, CA, USA) intraperitoneally at the time of immunisation and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive Transfer:

C57BL/6 mice were immunized subcutaneously over two sites on the lateral abdomen…followed by intraperitoneal injections of 250 ng of Bordetella pertussis toxin (List Biological Laboratories) on day 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE:

On days 0 and 2 mice were injected with 250ng of pertussis toxin (List Biologicals).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials for Experiments:

BoNT/A LC and HC were purchased from List Biological Laboratories inc. …

Biotinylation of BoNT/A HC and BoNT/A LC

BoNT/A LC (30 g) and BoNT/A HC (50 g) were biotinylated using the ChromaLinkBiotin One-Shot Antibody Labeling Kit according to Solulinks instructions. Two parameters were necessary to determine the concentration of the proteins: the mass extinction coefficient (E1%) for BoNT/A HC and LC, and their absorbance at 280 nm. The E1% of BoNT/A HC was reported to be 17.1 by Weatherly, G. T. et al [22]. E1% value of BoNT/A LC was roughly estimated to be 10. The biotin molar substitution ratio of biotinylated BoNT/A LC and biotinylated BoNT/A HC was calculated to be 2 and 4.3, respectively, using the MSR calculator of Solulink. The total biotin-labeled BoNT/A HC recovered after biotinylation was 28.3 g and the total labeled BoNT/A LC was 43.9 g. …

• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant
• Product #612A – Botulinum Neurotoxin Type A Heavy Chain, Recombinant, Binding Domain

…Recombinant protective antigen (PA) of anthrax and recombinant lethal factor (LF) were purchased from List Biologicals (Campbell, CA, USA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…100 ng/mL cholera toxin (List Biological, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell transfers and immunizations:

CD4+ T cells were enriched from wt or ICOS2/2 OT-II mice by MACS (Miltenyi). B cells from B1-8hi-GFP mice were enriched by depletion of non-B cells and k L chain B cells by MACS. … Mice were injected i.p. with…or 2 mg cholera toxin (CT; LIST Biological) in PBS.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Ultra pure TLR4 ligand lipopolysaccharide (LPS) was purchased from List Biological Laboratories (Campbell, California, USA). …

Author indicated the following LPS used:

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Reagents:

Lipopolysaccharide (LPS) was purchased from List Biologicals (Campbell, CA). …

Results – Acylcarnitine chain-length effects on IL-6 production:

…Furthermore, in experiments not shown, LPS treatment of cells failed to elicit an AK response despite the expected robust IL-6 response.

Author has indicated that List Labs Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4 was utilized for this research.