Bacterial Toxin Research Citations

Stereotaxic Injections of Tracers, OX-SAP:

Under chloral hydrate anesthesia (7% in saline, 350mg/kg), a fine glass pipette containing the tracers (1.0% Cholera toxin subunit B (CTB, List Biological), FG (5%), orexin-saporin (Advanced Targeting Systems) was lowered to the pre-calculated targets. Injections of 3100nl volumes were made over 10min by an air pressure system. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Pertussis toxin and cholera toxin were purchased from List Biological Laboratories, Inc. (Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and clinical scoring:

Age and sex matched mice between 8 and 12 weeks of age were induced for EAE by subcutaneous immunization with…and intraperitoneal (i.p.) administration of pertussis toxin (200 ng; List Biological Laboratories) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PA and LF were obtained from List Biological Laboratories, Inc. ,C ampbell, USA. The cells were incubated at 37 8 Ca nd 5% CO 2 for 3h. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Stereotaxic Injection and Fiber Optic Implantation into the Mouse Brain:

Strains of mice used for experiments were wild-type C57BL/6J, CD-1, Oxt^Cre/+, and Oxtr^f/f. 

Tissue Slice Preparation and Immunohistochemistry:

The prepared slices were labeled with the following primary antibodies:  goat anti-CTB (List Biological laboratories, 703, 1:500),

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Immunohistochemistry:

Immunohistochemical labeling was performed on cryostat (12 m) or vibratome (70 m) sections as described (Srmeli et al., 2011). Antibodies used in this study were as follows: goat anti-CTB (List Biological Laboratories),

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

Induction of MOG EAE was done as previously described.[29, 30] Shortly, 8 to 9-week-old female C57BL/6 mice were immunized with an emulsion containing 300 µg of MOG35-55 in phosphate-buffered saline and an equal volume of complete Freund’s adjuvant containing 5 mg/mL H37RA (Difco Laboratories, Detroit, MI). The inoculum (0.2 mL) was injected subcutaneously into the left flank. One hundred nanograms of pertussis toxin (List Biological Labs, Campbell, CA) in 0.1 mL phosphate-buffered saline was also injected i.p. on day 0 and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Pertussis toxin (200 ng per mouse; List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally on the day of immunization (day 0) and again 2 days later. Mice were monitored daily for clinical signs and scored as follows: 0, no disease; 1, tail paralysis; 2, paraparesis; 3, paraplegia; 4, paraplegia with forelimb weakness or paralysis; 5, moribund or dead animals.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Pertussis toxin (List Biological Laboratories, …was given at 200 ng per mouse on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

CNS inflammation was induced using a well-established model of EAE…In brief, groups of 10-wk-old age- and sex-matched mice were s.c. injected in the flank with 100 mg MOG₃₅₅₅ peptide (Genemed)… The same day and 2 d later, 200 ng pertussis toxin (List Biological Labs) in 200 µl physiological saline was injected i.v.

For Foxp3^DTR/+ mice, 8-wk-old females were injected i.p. on days 10, 7, and 4 with 500 ng diphtheria toxin (List Biological Labs). Then EAE was induced as described above.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae