Citations

EAE:

… Mice were also injected intraperiton eally with 200ng of pertussis toxin (List Biological Laboratories) on day 0 and day 2. Clinical signs of EAE were recorded daily using a 0 to 4 scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Immediately after immunization, and on day 2, the mice received intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in phosphate-buffered saline (PBS). PLP-induced EAE in SJL mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EC sensitization of mice and secondary site challenge:

… Mice were subjected to two rounds of EC sensitization with 100 µg of OVA on the first week, followed by a 2-week rest period. This cycle was repeated three times and, at the end of the third cycle, mice were challenged by intradermal (i.d.) injection in the ear of 20 µl of 1:1 mixture of 25 µg of OVA_323-339 peptide in PBS with 100 ng of pertussis toxin (PT; List Biological Laborarories, Inc.) and incomplete Freund adjuvant (IFA; Sigma). PBS-PT-IFA was injected in the second ear of the same mouse as control. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

For active EAE induction, mice were immunized in two sites on the hind flanks…Pertussis toxin (List Bio Lab) was administrated intraperitoneally at the dosage of 500 ng per mouse on day 1 and day 8. For single MOG_35-55/CFA immunization, the similar procedure was performed on day 0 and 1 only.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Pertussis toxin (200 ng per mouse; List Biological Laboratories) in PBS was administered i.p. on the day of immunization and again 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria toxin (DT) was administered to DTR mice genetically engineered to express the human diphtheria toxin receptor selectively in hair cells (Tong et al., 2011; Golub et al, 2012). A 50g/Kg dose of DT (List Biological Laboratories, Inc. #150) was delivered via intramuscular injection to four week old DTR mice. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

…The SEB and protective antigen (PA; List Biological Laboratories, Campbell, CA, USA) were fluorescently tagged using an amine reactive DyLight 488 …

MMS sorting:

In target sorting, the streptavidin-depleted library was resuspended in 1ml PBS and was incubated with 600nM SEB-biotin for 45min. After incubation, the cells were centrifuged at 3000g for 5min and resuspended in 1ml PBS containing 110⁹T1 streptavidin beads. After 45-min incubation with the magnetic beads, the cells were loaded onto the MMS and separated using the MMS positive selection program. The bacterial cells selected as SEB positive binders by the MMS were plated in serial dilutions to determine the resultant library diversity, and the remaining cells were grown overnight in LB-Cm²⁵. In the second, third, and fourth rounds of sorting, the SEB concentration was decreased from 600nM in round one to 300nM in round two, 150nM in round three, and 75nM in round four. …

Off-Cell affinity and specificity:

After blocking, a 45 min binding step was performed with each protein dissolved in PBST and serially diluted across each well in the 96-well plate: SEB-HRP initial concentration 14 nM, Strep-HRP initial concentration 250 nM, HRP initial concentration 200 nM, and PA-HRP initial concentration 100 nM. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Immunohistochemical analysis was performed using biotin conjugated cholera toxin B subunit (List Biological Laboratories, Inc.) for detection of GM1 gangliosides, …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… After topical application of tetracaine hydrochloride to the cornea, they received a unilateral intraocular injection of cholera toxin subunit B (CTb) (List Biological Laboratories, Inc., Campbell, CA). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Twelve days after the optic nerve was crushed, the operated animals were anesthetized with a 7% chloral hydrate solution, and 3 µl 1% cholera toxin B subunit solution (CTB; List Biological Laboratories, Campbell, CA) was intravitreally injected. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt