Bacterial Toxin Research Citations

Culture media and supplements:

… The culture medium was supplemented with 250 ng/ml amphotericin B solution (HyClone), 20 ng/ml cholera toxin (List Biological),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Methods:

… Adult male SpragueDawley rats (290360 g; Harlan) under anesthesia (1 ml/kg body weight of 50 mg/ml ketamine and 10 mg/ml xylazine, intramuscular) received single, iontophoretic injections of a mixture of 2.5% P. vulgaris leucoagglutinin (PHAL; Vector Labs) and 0.25% cholera toxin B subunit (CTB; List Labs) targeted stereotaxically to the LHAjv region (Swanson, 2004). … For IHC detection of tracers, the sections were incubated in primary antibodies directed against either CTB (1:10,000; goat, List BioLabs) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… An incision was made in the neck, and the Xth cranial nerve was isolated from the carotid artery and injected 2 cm distal from the head one to two times with the neuronal tract tracer cholera toxin B (CTB, 1% in dH 2 O, … List Biological Laboratories, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of diarrhea:

Cholera diarrhea was induced by oral administration of 25 µg cholera toxin (List Biological Laboratories, Campbell, CA)⁴⁴. Fifteen hours later, we examined the water volume in the intestinal lumen.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and Evaluation of EAU:

Lrrk2 (-/-) mice and their WT controls were immunized with bovine IRBP (150 g) and human IRBP peptide 120 (200 µg), emulsified with complete Freunds adjuvant (CFA) containing 2.5 mg/ml killed Mycobacterium tuberculosis (DIFCO, Detroit, MI), administered subcutaneously in a volume of 0.2 ml. In addition, the mice were injected intraperitoneally with 0.2 µg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS derived from Salmonella typhimurium from List Biological Laboratory (Campbell …

Placental macrophage and THP-1 cell treatment:

… For COX-2 selective inhibition experiments, cells were pretreated with 1 M NS-398 for 30 min, and then treated for 24 h with solvent control or 180 M MEHP with or without 1 M NS-398. Effective inhibitor concentrations were identified based on preliminary concentration response curves in THP-1 cells, using 500 ng/mL LPS as an inducer of COX-2 (data not shown). …

• Product #225 – LPS from Salmonella typhimurium

Immunohistochemistry:

After post-operative survival times of three and five months, mice were terminally anaesthetized and perfused with room temperature saline, followed by a fixative solution containing cooled 4% w/v formaldehyde and 14% v/v saturated picric acid in PBS (pH 7.35 7.45). … The anti-cholera toxin subunit B (CTB, List biological laboratories) antibody was incubated for 4h at room temperature and…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE Induction:

EAE was induced by immunization…followed by two doses of 150 ng pertussis toxin (List Biological Laboratories) i.p. on day 2 and day 4 post-immunization. Mice were monitored for signs of disease as previously described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and cell transplantation:

… The mice also received 350 ng pertussis toxin (List Biological Laboratories Inc, CA, USA) intraperitoneally at the time of immunisation and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive Transfer:

C57BL/6 mice were immunized subcutaneously over two sites on the lateral abdomen…followed by intraperitoneal injections of 250 ng of Bordetella pertussis toxin (List Biological Laboratories) on day 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)