Bacterial Toxin Research Citations

EAE Induction:

… with 200 µg of MOG 35-55 peptide emulsified with complete Freund’s adjuvant (CFA) containing 500 µg of Mycobacterium tuberculosis H37RA (BD Biosciences) on day 0 and were ip injected with 500 µg of pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Mice were immunized subcutaneously with MOG 3555 peptide (UCLA Biopolymers Core) emulsified in Complete Freunds Adjuvant (CFA, Difco) with M. tuberculosis (Difco). Additionally, mice were injected with Pertussis toxin (PT, List Biological Laboratories) i.p. on days 0 and 2 following immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin-induced monocyte depletion mouse model:

Itgam(CD11b)-DTR mice were obtained from Jackson Laboratories. CD11b-DTR and C57BL/6 WT mice were injected i.p. with 100 l of diphtheria toxin (List Biological Laboratories, Inc. Campbell, CA) at a concentration of 0.02 g per gram of mouse weight. Two days after PBS or CpGB DNA pump insertion, skin was collected for RNA isolation or immunofluorescence.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… A small cut was made in the dorsal sclera, through which CTB (20 µl of 0.83% in PBS with 2% dimethylsulfoxide [DMSO]; List Biological, Campbell, CA) was injected into the eye’s vitreous body with a Hamilton (Reno, NV) syringe. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunohistochemistry:

In BDNF-MSCs treated SH animals, cholera toxin subunit B (CTB) (List Biological Laboratories, Campbell, CA) was used to retrogradely label phrenic motoneurons by intrapleural injection at 3 days before the terminal experiment, …Spinal cord sections were blocked with 10% donkey serum in 0.3% Triton tris-buffered saline (TBS), followed by overnight incubation with CTB antibody (goat polyclonal, #703; List Biological Laboratories) and …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

MOG79-96 induced EAE:

… Mice were given an additional intraperitoneal (i.p.) injection of 200 µg Pertussis Toxin (PTx) (List Biological Laboratories, Surrey, UK) on day of immunisation and 2 days post immunisation (d.p.i.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse immunohistochemistry – goat anti-CTB (1:4,000, List Laboratories) was used. Behavioural Testing Analysis – Diphtheria Toxin (List Biological Laboratories) was used.

Reagents:

Ultrapure LPS (from Escherichia coli 0111:B4) was from List Biological Laboratories Inc. …

RESULTS – Multiple TLR ligands and cytokines stimulate HC-TNFR1 shedding during polymicrobial sepsis:

We previously showed that HC-TNFR1 shedding is stimulated by the TLR4 ligand lipopolysaccharide (LPS) in vitro and in vivo (10); however, during polymicrobial sepsis, multiple TLR ligands and cytokines are involved in propagating the inflammatory response. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Ubiquitin ELISA:

… A polystyrene plate was coated with goat anti-CTB antibodies (List Biological, Campbell, CA), blocked with PBSTM and crude leaf extracts were added.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction and evaluation of EAE:

To induce relapse EAE, SJL.J mice were immunized with 150µg of PLP₁₃₉₁₅₁ in CFA containing 5mg/ml¹ heat-killed Mycobacterium tuberculosis. For both types of induction, on the day of immunization and 48h later, the mice were also injected i.v. with Pertussis toxin (200ng per mouse; List Biological Laboratories, Campbell, CA) in PBS. Mice were examined daily for EAE disease symptoms, which were scored using a…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)