Citations

LPS-induced TNF- release ex vivo:

Samples of EDTA blood (Cohort II) were stimulated with 1 ng/ml LPS (List Laboratories, Campbell, CA, USA) in Modified Eagle Medium (Invitrogen, Grand Island, NY, USA) control or medium only, and incubated in pyrogen-free 1.5-ml tubes for 4 h at 37C, rotating end-over-end, prior to collection of the extracellular medium for subsequent TNF- ELISA (R&D Systems, Minneapolis, MN, USA or BD BioSciences, San Jose, CA, USA).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

… LPS from Escherichia coli O111:B4 was obtained from List Biological Laboratories (Campbell, CA, USA). …

ELISAs:

Isolated BMCs were cultured in the presence of M-CSF (20 ng/ml) + G-CSF (10 ng/ml). On Day 5, cells were sorted using a FACSAria III cell sorter, washed with PBS twice, and cultured in a 96-well plate for 2 h. Subsequently, cells were treated with 100 ng/ml LPS, and cytokine levels were determined in cell culture supernatants using commercially available quantitative ELISA kits from eBioscience. …

• Product #201 – LPS from Escherichia coli O111:B4

Mice:

… Mice were injected with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally on the day of immunization and 2 days after immunization. Mice were assessed daily for clinical signs of EAE according to the following scale designated as EAE clinical score: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Control mice were injected sc bilaterally with an emulsion of PBS, 8 mg/mL H37RA and incomplete Freund’s adjuvant. 100 ng Pertussis toxin (List Biological Laboratories, Inc, Campbell, CA, USA) was administered ip on days 0, 1 and 2 to induce EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For EAE-immunized mice, the adjuvant pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA)was injected intravenously on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental allergic encephalomyelitis, evaluation of symptoms, and administration of peptides:

…On day 1, SJL/J mice were injected with 300µg/mouse bovine myelin basic protein (Invitrogen) that was emulsified in Complete Freund’s Adjuvant with…and injected subcutaneously into 2 sites at the base of the tail along with 400ng/mouse of pertussis toxin (List Biological Laboratories, Inc.) in PBS i.p… On day 3, the pertussis toxin injection was repeated.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (400 ng) (List Biological Laboratories) in PBS and 50 mM NaCl was administered ip on the day of immunization and again after 24 h. For the treatment of EAE, the dose/route/frequency of administration drug was determined through pre-experiments. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For Langendorff perfusion experiments, pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA, USA)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE disease severity is not altered in Cnr2tmZim mice. EAE was induced in Cnr2tmZim mice by immunization with 300 µg MOG3555 emulsified in complete Freunds adjuvant containing 4 mg/ml Mycobacterium tuberculosis… Pertussis toxin (200 ng) (List Biological Laboratories, Campbell, CA) was given i.p. on days 0 and 2 post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatments:

EAE was induced by immunization of female mice with MOG3555 peptide emulsified in CFA (Difco Laboratories) at a dose of 100 µg (C57BL/6 and F1 mice) or 150 µg (NOD mice) per mouse, followed by the administration of pertussis toxin (150 ng per mouse; List biological laboratories, Inc.) on days 0 and 2 as described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)