Bacterial Toxin Research Citations

Immunization.

Mice were immunized weekly for 6 weeks by oral gavage with 5 mg of ground blanched peanut (Western Mixers Produce & Nuts) or 1 mg of NP-Ovalbumin (LGC Biosearch) with 10 µg CT (List Biologicals, Lot: #10167A2 and #10165A1) in 200 µl of 0.2 M sodium bicarbonate buffer per mouse based on a protocol modified from (10). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE]

EAE was induced in 7- to 13-wk old female mice as described previously.81 Briefly, mice were immunized subcutaneously with 150 μg MOG35-55 (MEVGWYRSPFSRVVHLYRNGK, EZBiolab) in PBS and complete Freund’s adjuvant (BD Biosciences, cat # 263910) containing 4 mg/mL M. Tuberculosis H37Ra (BD Biosciences, cat # 231141) with or without intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories, cat # 180) on day 0 and day 2. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Diphtheria Toxin (DT) List Biological Laboratories Inc Cat# 150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The pertussis toxin was purchased from List Biological Laboratories(Campbell, CA, U.S.A.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3 Experimental autoimmune encephalomyelitis

Mice were induced with experimental autoimmune encephalomyelitis (EAE) following a protocol well established in the laboratory (Brambilla et al., 2011; Madsen et al., 2020). Briefly, on day 0 and 2, pertussis toxin (PTX, List Biological Laboratories, #181) diluted in PBS was injected intraperitoneally (500 μg/mouse). On day 1, myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55, Bio-Synthesis, #A9349-1) emulsified in complete Freund’s adjuvant was injected subcutaneously (0.33 mg/mouse). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

4.2. EAE Model Preparation

The detailed methods used for the EAE procedure are as described previously [25]. Briefly, EAE was induced by immunization of mice with 200 mg of MOG35–55-CFA solution (Myelin Oligodendrocyte Glycoprotein peptide in Complete Freund’s Adjuvant) injected subcutaneously near the inguinal lymph nodes and the axillary lymph nodes on the left side of the mouse, followed by an intraperitoneal injection administering 500 ng of Pertussis toxin (95008-6906; List Biological Laboratories Inc., Campbell, CA, USA) in phosphate-buffered saline (PBS), pH 7.4, on days 0, 2, and 7. Repeat intraperitoneal injections were always carried out on the opposite side of the previous injection. Mice were anaesthetized with a 2–2.5% isoflurane with an oxygen flow of 2 L per minute to minimise pain and distress. Tonabersat was administered via single daily intraperitoneal injections at doses of 0.2, 0.4 and 0.8 mg/kg, either following early dosing (days 3–15 post-inoculation) or late dosing (days 8–15 post-inoculation) regime. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Evaluation of i.v. administration of BLG-p(Man) in a mouse model of BLG sensitization

This BLG sensitization model was adapted from ref. 27 and 28. Beginning at 2 weeks of age, SPF C3H/HeJ mice were treated with a
mixture of antibiotics (Abx). For the first week before weaning, mice were treated with 100 mL Abx solution once daily, including kanamycin (4 mg/mL), gentamicin (0.35 mg/mL), colistin (8500 U/mL), metronidazole (2.15 mg/mL), and vancomycin (0.45 mg/mL)
(Sigma-Aldrich). After weaning, mice were given drinking water supplemented with a 50-folded dilution of the same Abx solution
except vancomycin, which was maintained at 0.45 mg/mL, throughout the duration of the experiment. Prior to weaning, on day 1
and day 5 after first oral gavage of Abx, the mice were i.v. administered with PBS, or 2 mg BLG in the form of unmodified (free)
BLG or BLG-p(Man) per 10 g body weight. Right after weaning, mice were sensitized weekly for 5 weeks by intragastric gavage
with BLG (20 mg per 10 g body weight) (Sigma-Aldrich) and cholera toxin (CT, 10 mg per 10 g body weight) (List Biologicals, Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Recombinant antigens

Pertussis toxin (salt free) was obtained from List Labs (Campbell, CA, USA), …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

SF stimulation

SF were stimulated with human recombinant TNF (10 ng/ml; R&D Systems), human recombinant IL1β (1 ng/ml; R&D Systems), lipopolysaccaride (LPS) from Escherichia coli J5 (100 ng/ml; List Biological Laboratories), polyI-C (PIC) (10 μg/ml; InvivoGen), bacterial lipopeptide (bLP) palmitoyl-3-cysteine-serine-lysine-4 (300 ng/ml; InvivoGen) or human recombinant TGFβ (10 ng/ml; R&D Systems) for 24 h. …

• Product #301 – LPS from Escherichia coli J5 (Rc)

EAE induction

For EAE induction, mice were injected subcutaneously at two sites on the back with 200 mg MOG35–55peptide (synthesised by Toray Research Center, Tokyo, Japan) and 1 mg heat-killed Mycobacterium tuberculosis H37RA emulsified in complete Freund’s adjuvant (Difco, KS, USA). Four hundred nanograms of Pertussis toxin (List Biological Laboratories, CA, USA) were injected intraperitoneally on days 0 and 2 after immunisation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)