Bacterial Toxin Research Citations

EAE Induction:

Experimental autoimmune encephalomyelitis was induced in mice by active immunization with MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK), of murine origin… These mice were also injected i.p. with 500 ng pertussis toxin ([PTX] List Laboratories, Campbell, CA), in PBS, pH 7.4, on D0 and D2 after the first immunization (referred to as the MOG-CFA/PTX group).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction in mice:

… on day 0. 6 and 48 h later, the mice were administered i.v. with 500 ng of purified Bordetella Pertussis toxin (List Biological Laboratories, Campbell, CA). Disease severity was monitored according to the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

… Mice were immunized s.c. with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in complete Freund’s adjuvant, in a volume of 0.1 ml/mouse over the right-draining inguinal and axillary lymph nodes…Pertussis toxin (500 ng/mouse; List Biological Laboratories, Campbell, CA) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Acute Optic Neuritis of EAE:

…For EAE-immunized mice, the adjuvant pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of immunization and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigen-induced arthritis:

… Simultaneously, 1 µg of pertussis toxin (List Biological Laboratories Inc, Campbell, CA, USA) was injected intraperitoneally as an additional adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Corneal afferent tract tracing and capsaicin application techniques for immunocytochemistry studies:

2.3.1. Cholera toxin B subunit

(CTb; 1% solution in 0.1 M phosphate buffer (PB); List Biological Laboratories, Inc., Campbell, CA) was used as an anterograde tracer to identify central projections of corneal afferents into the ventrolateral dorsal horn of trigeminal nucleus caudalis (Vc) as previously described …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Animal Surgery and EEG/EMG Implantation:

… Injections of… or CTB, List Biological, Campbell, CA) were administered directly into the brain using a fine glass pipette (1 mm glass stock, tapering slowly to a 10-20 µm tip) connected to an air compression system. A series of 20-40psi puffs of air were used to deliver the compounds into and with the following coordinates and volumes: …, 33nL 1% CTB; …16.5nL 1% CTB…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Purity and characteristics of the Purified C Fragment were determined by HPLC and Western Blot, in comparison with Standard C Fragment purchased from List Biological Laboratories Inc., USA. …

• Product #193 – Tetanus Toxin C-Fragment from Clostridium tetani

TNA Assay:

The TNA Assay measures the levels of anthrax lethal toxin neutralizing antibody using an in vitro cytotoxicity assay. The assay was originally validated at the CDC, but was then transferred and validated at Battelle, where the testing of these serum samples occurred[8, 9]. Briefly, microtiter cell plates were seeded with J774A.1 cells and allowed to adhere. In separate microplates a mixture of recombinant protective antigen (rPA, List Biological Laboratories, Inc., Campbell, California, Cat. No. 171B) and recombinant lethal factor (rLF, List Biological Laboratories, Inc., Campbell, California Cat. No. 172B) was added to serial dilutions of the test samples and controls and incubated prior to transfer to the cell plate. The final concentration of rPA was 0.05 g/mL and the final concentration of rLF was 0.04 g/mL. MTT was then added to the cell plates to allow viable cells to reduce the MTT dye. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis