Bacterial Toxin Research Citations

Induction of EAE:

…Female SJL/J mice were immunized by subcutaneous injection into the flank regions with emulsified PLP139-151 (50 nmol/mouse). Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS was injected intravenously (100 ng/mouse/day) on the day of immunization and 2 days later. Mice immunized by subcutaneous injection of emulsion without PLP139-151 served as normal controls. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Please note that a goat anti-CTB antibody is also available from List Biological Labs and can be then used as an alternative to the rabbit anti-CTB antibody. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… And pertussis toxin 200 ng (List Biological laboratories, USA) on the day of immunization and on the second day after immunization. After immunization, daily weight and clinical symptoms of the mice were measured. The progress of the EAE is …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Shiga toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Shiga toxin was utilized.  List Labs provides Product #161 – Shiga Toxin 1 from Escherichia coli and Product #162 – Shiga Toxin 2 from Escherichia coli.

EAE induction and treatments:

EAE was induced by immunization female mice with MOG₃₅₅₅ peptide emulsified in CFA (Difco Laboratories) at a dose of 100µg (C57BL/6 and F1 mice) or 150µg (NOD mice) per mouse, followed by the administration of Pertussis toxin (150 ng per mouse; List Biological Laboratories, Inc.) on days 0 and 2 as described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Preparation of primary mouse corneal epithelial cells:

We followed the methods described previously to conduct the primary culture of the corneal epithelial cells by corneal discs obtained from freshly killed Kcnj10^/ (KO; 710 days old) and the corresponding wild-type littermates… The pellet cells were resuspended with Epilife…with 100 ng/ml cholera toxin (List Biological Laboratories, Campbell, CA) and 1 antibiotic. The subcultured primary cells at passage 2 were used for electrophysiology studies. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Stimulants of ROS production included PMA (Sigma-Aldrich) at 10 7 M, Salmonella typhimurium LPS (List Biologicals Laboratories, Inc., Campbell, CA) at 1 g/ml, …

• Product #225 – LPS from Salmonella typhimurium

Experimental allergic encephalitis induction:

Experimental allergic encephalitis (EAE) induction experiments were performed as described in our previous study (21). …Some experiments were performed using 100 mg MOG3555 plus 100 mg zymosan in IFA. Mice also received 250 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2 postimmunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE):

Ten mice were randomly selected to be immunized with 50 µg MOG₃₅₅₅ peptide emulsified (1:1) in incomplete Freunds adjuvant (IFA) and Mycobacterium tuberculosis. Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA, USA) was injected intravenously on the day of MOG₃₅₅₅ immunization and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Serological analysis:

Anti-PT IgG antibodies were analysed using a validated in-house enzyme-linked immunosorbent assay (ELISA) slightly modified from previous publications [15,16]. Briefly, PT (List Biological labs, CA, USA) was coated to 96 wells micro-titer plates at 1 µg/ml in 0.05 M bicarbonate buffer pH 9.6 for 48 h at 4° C. Blocking was performed with 250 µL 1% powdered skimmed milk (Oxoid, UK) in PBS for 30 min at room temperature. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)