Citations

Study animals:

… Individual foals were randomly assigned to the following groups: 1) EBRE 1 group (n=9) which received 21010 CFUs of R. equi inactivated by 4 kGy of e-beam radiation  in 100 ml of saline adjuvanted with 100 g of
cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA); 2) EBRE 2 group (n=10) which received 11011 CFUs of R. equi inactivated by 5 kGy of e-beam radiation in 100 ml of saline adjuvanted with 100 g of CTB; 3) Saline (negative) control group (n=9) which received 100 ml of saline adjuvanted with 100 g of CTB; and, 4) LVRE (positive) control group (n=6) which received 11010 CFUs of live R. equi in 100 ml of saline. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Engraftment of mice with human PBMCs:

Female C.B-17 SCID mice (Taconic) were maintained in microisolator cages (Innovive) and used at 10 weeks of age. The engraftment of human PBMCs into SCID mice was performed essentially as previously described ([25, 26]). … On day 10, 10 mice in each group were injected intraperitoneally with 15 g of tetanus toxoid (List Biological Laboratories) in PBS. On the days indicated, blood was collected, and serum was assayed for human IgM, IgG2, and anti-tetanus IgG (IBL). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Cytotoxicity assay:

PA and LF were purchased from List Biological Laboratories, Inc., …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization and immunogenicity studies:

For intranasal immunizations, 4 µg of the indicated recombinant protein with 1 µg cholera toxin (CT; List Biological Laboratories Inc.) per dose was administered in a 20 µl volume in sterile phosphate-buffered saline (PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tissue Processing, Analysis, and Immunohistochemistry:

Nucleus ambiguus.

…The sections were then incubated in goat anti-CTB antiserum (1:25,000; List Biologicals) in TPBS-Triton containing 10% NHS for 3 days at room temperature. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and Diets:

Male APOE3L.CETP mice were 1014 weeks old…

… Groups were additionally treated with one of the following treatments until week 16: LPS from Salmonella minnesota R595 (lot. no. 30446A1; List-Biological Laboratories, Campbell CA, USA) by minipump at 5g per day; recombinant murine IL-1…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Briefly, BALB/c mice were injected three times intraperitoneally with 100 or 150 µl of the viral suspension (1.3 10 10 copies/ml) in PBS on days 0, 14 and 53 and with 0.1 µg of inactivated pertussis toxin (Pertussis Toxin Salt-Free; List Biological Laboratories, Inc., Campbell, CA …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Toxin injection:

Mice were injected intraperitoneally with PTX (20 µg/kg), PTX-A (5.3 µg/kg), PTX-B (14.5 µg/kg), PTX^mut (20 µg/kg), … The toxins were purchased from List Biological Laboratories, … In most experiments, mice were given a single injection and killed 3 or 6 h later. In indicated experiments, they were given 2 injections, 2 days apart, and killed 24 h after the last injection.

Bone marrow transplantation:

… Chimeric mice were injected with PTX 2 months after transplantation.

EAE induction by active immunization:

Mice were subcutaneously injected into both flanks with a total of 200 µl of emulsion containing 300 µg of MOG peptide 3555 (Feldan) dissolved in saline and mixed with an equal volume of complete Freund’s adjuvant containing 500 µg of killed Mycobacterium tuberculosis H37 RA (Difco Laboratories). The mice were also injected intraperitoneally with 20 µg/kg of PTX immediately and 2 days after immunization.

Ex vivo stimulation of leukocytes:

Peritoneal leukocytes were harvested by flushing the peritoneum of naive mice with 5 ml D-PBS. Cells were pooled in DMEM supplemented with 2% FBS, transferred in 24-well plates at a density of 210⁶ cells per well in 500 µl containing or not 500 ng/ml PTX, PTXmut or heat-inactivated PTX (heated at 80C for 30 min). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

Mice were injected subcutaneously in both flanks with 200 µL of inoculum containing 100 µg MOG₃₅₅₅ and 0.5 mg Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI) in incomplete Freund’s adjuvant. Pertussis toxin (200 ng; List Biological Laboratories Inc., Campbell, CA) was injected intravenously on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… The animals also received 150 ng of pertussis toxin (PT) (List Biological Laboratories) intra-peritoneally (IP) on day 0 and day 2. The animals were followed daily, and clinical signs of EAE were assessed on a daily basis according to the following scores: 0, no disease; 1, loss …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)