Citations

Induction of EAE in DR2-Tg and MBP-TCR/DR2-Tg mice

…In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 postimmunization (75 and 200 ng/mouse, respectively).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

The mice also received two 400 ng intraperitoneal injections of pertussis toxin (List Biological Laboratories,… immediately and 48 h later in 100 µL of phosphate buffered saline (PBS).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

In addition, mice were treated with pertussis toxin (PTX; 200 ng, List Biological Laboratories) administered intraperitoneally on the day of immunization (day 0) and on dpi 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE disease models:

On days 0 and 2, 50 µg pertussis toxin (List Biological Laboratories) was given i.v. Subsequently, these mice were treated with KN-93 or PBS twice a week.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo experiments

EAE was induced by subcutaneous injection of 150 µg MOG peptide (amino acids 3555), in 0.1 ml of CFA emulsion containing 400 µg Mycobacterium tuberculosis (Difco), into wild-type or Traf5^/ B6 mice on day 0. The mice received intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2. Clinical signs of EAE were assessed according to the following…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The nasal tissue-derived cells were immunostained without in vitro culture or stimulation. The frozen PBMCs were thawed for phenotype analysis after stimulation with SEB (List Biological Laboratories Inc., Campbell, CA) in 96-well round-bottom plates. …

…Ovalbumin was purchased from Greer Source Materials (Lenoir, NC). Cholera toxin B (CT) utilized as an adjuvant was purchased from List Biological Laboratories (Campbell, CA). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Animals:

…Five animals received CTb (highly purified B subunit isolated from Vibrio cholerae type Inaba 569B, product 103B; List Biological, Campbell, CA) injections into the CT in the middle ear;

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… and cholera toxin subunit B [CTb; List Biological, Campbell, OR; 12%]) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Viral transneuronal tracing experiments:

The viral transneuronal labeling experiments followed the same protocol as described in a previous publication from our laboratory (39). Sprague-Dawley male and female rats (n = 60) were anesthetized with pentobarbital sodium (50 mg/kg ip) and placed in a stereotaxic apparatus. The skull was leveled, and a craniotomy was performed. A glass micropipette (25-m tip diameter) containing a mixture of the Bartha strain of pseudorabies virus (PRV; K. Platt, Iowa State University, Ames, IA) and cholera toxin -subunit (CTb; 0.1% salt free; no. 103B, List Biological, Campbell, CA) was advanced into the NAc with a micromanipulator. A 40-nl injection of a mixture of the Bartha PRV (5 l; titer = 1 10⁸ plaque-forming units/ml) and 0.1% CTb (2 l) made in a 0.02 M potassium phosphate buffer was delivered over 15 min using a hand-held air pressure injection system. …

To evaluate the injection site, forebrain sections at the level of the NAc were reacted with a polyclonal goat anti-CTb (1:25,000, no. 703; List Biological), as described previously (39). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt