Bacterial Toxin Research Citations

The concept of a soluble SNAP-25 FRET substrate was further expanded with the design of a 13-mer peptide containing a terminal FITC fluorophore and DABCYL quencher; this product, SNAPtide, was commercialized by List Biological Laboratories, Inc. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

… After final behavioral testing, animals were anesthetized as described above and nerves were traced using a 3 µL injection of Cholera Toxin B subunit (CTB; 1% CTB in sterile diH 2 O; List Biological Laboratories, Campbell, USA) 10 mm distal to the crush site (63). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents and Antibodies:

LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA). …

Analysis of in Vitro CSR:

Splenic B cells were stimulated with LPS (20 g/ml) or anti-CD40 antibodies and the additional reagents indicated below. …

RNA Sequence:

Total RNA were purified from LPS-stimulated (20 g/ml) splenic B cells on day 3 by RNeasy…

ChIP Assay:

Splenic B cells were activated with LPS plus IL-4 for 3 days. …

• Product #201 – LPS from Escherichia coli O111:B4

Inhibition of Cell Signaling Pathways:

… All inhibitors were obtained from Calbiochem, except for PTX (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA), and it was dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Sensitization and treatment protocol:

…Sensitization began when mice were 6 to 12 weeks old. Sensitized mice received, by gavage, a mixture of PT-gliadin (500 µg) and cholera toxin (CT) (25 µg) (List Biological Laboratories Inc.) once a week for 3 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For the experiment, the TA muscle on the immobilized side was injected with either 1 l saline (N = 5), or 1 ng tetanus toxin dissolved in 1 l saline (N = 7; List Biological Laboratories) at the time of joint fixation. …

• Product #190 – Tetanus Toxin from Clostridium tetani

Peptides and proteins:

… Recombinant protective antigen (rPA) was obtained from List Biologicals (Campbell, CA ) ….

 Vaccines and immunization:

… For anthrax and influenza protein vaccines used in Chapter 3, lipids were prepared with S100 lecithin (Lipoid GmBH, Bermany).  These vaccines were delivered via intramuscular injections of 25 L on each the right and left leg.  Each 50 L dose of anthrax vaccine contained 1 g rPA and adjuvants as indicated in figure legends.  …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

PN allergy model including antibody administration:

Intragastric gavage (Delvo, Biel/Bienne, Switzerland) of PN (Kraft, Northfield, IL) with CT (List Biologicals, Campbell, CA) was performed weekly for 4 weeks, with challenge by crude PN extract (Greer, Lenoir, NC) 1 week later.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antigens and adjuvants: Cholera toxin was from List Biological Laboratories.

Epicutaneous sensitization of mice:

Six- to eight-week-old female mice were anesthetized, and abdominal fur was removed with depilatory cream… SEB or cholera toxin were applied at a dose of 10 µg. In some experiments, antigens were applied to the ear pinnae. Mice were exposed weekly for a total of 6 exposures and challenged a minimum of 1 week after the last exposure.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae