Bacterial Toxin Research Citations

Materials and Reagents:

… Lipopolysaccaride (LPS) from Salmonella typhimurium was from List Biological Laboratories (Campbell, CA). …

Culture of Extraplacental Choriodecidual Membranes:

… LTA and LPS treatments were initially dissolved in water and diluted in DMEM/1% FBS. Following acclimation, …

• Product #225 – LPS from Salmonella typhimurium

… the Toll-like receptor [TLR]4 ligand lipopolysacharide [LPS], from Salmonella typhymurium, 0.1 g/mL, List Biological Laboratories (Campbell, CA …

• Product #225 – LPS from Salmonella typhimurium

Protein transfection:

… BMDMs were primed with 10 ng/mL of ultrapure LPS (List Biologicals) for 3 hours to induce pro-IL-1 expression prior to protein transfection, using 88 Profect-P1-lipid based protein delivery reagent (Targeting Systems) as previously described (10, 52); IL-1 secretion was determined by ELISA (Duoset R&D). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and antibodies:

…LPS was from List Biological Laboratories Inc. (Campbell, CA, USA). …

Real-time reverse transcriptionPCR:

BMDMs or RAW264.7 cells (110⁶ cells ml¹) were cultured up to 8h in the presence or absence of LPS, CpG-DNA, necrotic cells or enzyme-treated necrotic cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Pertussis toxin (PTX) List Biologicals 180217A1 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE:

… Mice were also injected intraperiton eally with 200ng of pertussis toxin (List Biological Laboratories) on day 0 and day 2. Clinical signs of EAE were recorded daily using a 0 to 4 scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Immediately after immunization, and on day 2, the mice received intraperitoneal injections of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in phosphate-buffered saline (PBS). PLP-induced EAE in SJL mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EC sensitization of mice and secondary site challenge:

… Mice were subjected to two rounds of EC sensitization with 100 µg of OVA on the first week, followed by a 2-week rest period. This cycle was repeated three times and, at the end of the third cycle, mice were challenged by intradermal (i.d.) injection in the ear of 20 µl of 1:1 mixture of 25 µg of OVA_323-339 peptide in PBS with 100 ng of pertussis toxin (PT; List Biological Laborarories, Inc.) and incomplete Freund adjuvant (IFA; Sigma). PBS-PT-IFA was injected in the second ear of the same mouse as control. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

For active EAE induction, mice were immunized in two sites on the hind flanks…Pertussis toxin (List Bio Lab) was administrated intraperitoneally at the dosage of 500 ng per mouse on day 1 and day 8. For single MOG_35-55/CFA immunization, the similar procedure was performed on day 0 and 1 only.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Pertussis toxin (200 ng per mouse; List Biological Laboratories) in PBS was administered i.p. on the day of immunization and again 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)