Bacterial Toxin Research Citations

IPEC-J2 cell culture conditions and bacterial adherence assays:

…To test for the effects of endogenous LT expressed by the bacteria on adherence in isogenic porcine-origin ETEC strains that had not demonstrably shown an effect of LT on colonization [14], inocula were prepared and directly applied to the IPEC-J2 cells, as described above. To test the effect of exogenous LT on adherence on these strains, LT (List Biological Laboratories) at a concentration of 1, 10, or 100 ng/ml per well was added (pre-incubation) 1 h prior to bacterial inoculation. To test the potential of GM1 ganglioside to mitigate the effects of LT on adherence, LT at a concentration of 10 ng/ml or 100 ng/ml was incubated with the cells 1 h prior to their inoculation with bacteria, and 10 ng/ml or 100 ng/ml GM1 ganglioside (Sigma) was applied to the IPEC-J2 cells at the time of bacterial inoculation. Three independent experiments were performed for each assay.

Test of effects of different carbohydrates on LT production and secretion:

To test the effects of different sugars on LT secretion, CAYE media containing glucose, sucrose or fructose at 0%, 0.25%, 0.5%, 1%, or 2% were prepared. Strains were cultured in different media as described above, with aliquots taken at 0, 2, 4, and 6 h post-inoculation (PI). These samples were centrifuged (2500g, 10 min), and supernatants taken to analyze for LT concentrations by GM1-ELISA as previously described [17]. Three independent experiments were performed for each assay. 

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Ultrapure E. coli O111:B4 LPS, List Biological Laboratories, 421, …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Cell Culture and Transfection:

BT-20, DLD-1, HEK293T, HCC1937, HeLa, MDA-MB-435, MDA-MB-231, NIH 3T3, and MCF7 cells were cultured in DMEM (Cellgro) supplemented with 10% fetal bovine serum (FBS; GIBCO). ZR-75-1 cells were grown in RPMI 1640 supplemented with 10% FBS. MCF10A cells were maintained in DMEM/Hams F12 medium with 5% equine serum (Cellgro), 10 mg/ml insulin, 500 ng/ml hydrocortisone (Sigma-Aldrich), 20 ng/ml epidermal growth factor (EGF) (R&D Systems), and 100 ng/ml cholera toxin (List Biological Laboratories).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Female A/J mice were vaccinated with rPA (1 mg; List Laboratories) formulated with Al(OH) 3 (200 m g), SMIP-7.10 (25 m g), or both.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

… Mice were also injected intraperitoneally with 400 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) on days 0 and 2. Clinical scores of EAE were evaluated as follows: 0, no signs; 1, paralyzed tail; 2, mild hind limb paresis; 3, moderate hind limb paralysis; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… To facilitate the efficiency of EAE induction, 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) were given intraperitoneally to each mouse on the day of immunization with MOG 3555 peptide and 48 h later (total 2 doses). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Recombinant human myelin oligodendrocyte glycoprotein (rhMOG) 1125 (rhMOG1125) was generated as previously described (5). EAE was induced by s.c. immunization at four sites on the back… On days 0 and 2, mice were injected i.p. with 300 ng pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

The concept of a soluble SNAP-25 FRET substrate was further expanded with the design of a 13-mer peptide containing a terminal FITC fluorophore and DABCYL quencher; this product, SNAPtide, was commercialized by List Biological Laboratories, Inc. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

… After final behavioral testing, animals were anesthetized as described above and nerves were traced using a 3 µL injection of Cholera Toxin B subunit (CTB; 1% CTB in sterile diH 2 O; List Biological Laboratories, Campbell, USA) 10 mm distal to the crush site (63). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents and Antibodies:

LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA). …

Analysis of in Vitro CSR:

Splenic B cells were stimulated with LPS (20 g/ml) or anti-CD40 antibodies and the additional reagents indicated below. …

RNA Sequence:

Total RNA were purified from LPS-stimulated (20 g/ml) splenic B cells on day 3 by RNeasy…

ChIP Assay:

Splenic B cells were activated with LPS plus IL-4 for 3 days. …

• Product #201 – LPS from Escherichia coli O111:B4