Bacterial Toxin Research Citations

Mice:

… Mice were injected with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally on the day of immunization and 2 days after immunization. Mice were assessed daily for clinical signs of EAE according to the following scale designated as EAE clinical score: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Control mice were injected sc bilaterally with an emulsion of PBS, 8 mg/mL H37RA and incomplete Freund’s adjuvant. 100 ng Pertussis toxin (List Biological Laboratories, Inc, Campbell, CA, USA) was administered ip on days 0, 1 and 2 to induce EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For EAE-immunized mice, the adjuvant pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA)was injected intravenously on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental allergic encephalomyelitis, evaluation of symptoms, and administration of peptides:

…On day 1, SJL/J mice were injected with 300µg/mouse bovine myelin basic protein (Invitrogen) that was emulsified in Complete Freund’s Adjuvant with…and injected subcutaneously into 2 sites at the base of the tail along with 400ng/mouse of pertussis toxin (List Biological Laboratories, Inc.) in PBS i.p… On day 3, the pertussis toxin injection was repeated.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (400 ng) (List Biological Laboratories) in PBS and 50 mM NaCl was administered ip on the day of immunization and again after 24 h. For the treatment of EAE, the dose/route/frequency of administration drug was determined through pre-experiments. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For Langendorff perfusion experiments, pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA, USA)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE disease severity is not altered in Cnr2tmZim mice. EAE was induced in Cnr2tmZim mice by immunization with 300 µg MOG3555 emulsified in complete Freunds adjuvant containing 4 mg/ml Mycobacterium tuberculosis… Pertussis toxin (200 ng) (List Biological Laboratories, Campbell, CA) was given i.p. on days 0 and 2 post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatments:

EAE was induced by immunization of female mice with MOG3555 peptide emulsified in CFA (Difco Laboratories) at a dose of 100 µg (C57BL/6 and F1 mice) or 150 µg (NOD mice) per mouse, followed by the administration of pertussis toxin (150 ng per mouse; List biological laboratories, Inc.) on days 0 and 2 as described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Deletion:

Deletion of Jun and JunB was verified by PCR. Similarly, 300 ng of Diphtheria toxin (DT, List Biological Laboratories, in PBS) was injected intraperitoneally into experimental mice according to the schemes indicated in the figures. For LC depletion, DT was applied every third day, and for pDC depletion, every other day.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Anatomical tracer injections:

…Cholera toxin B subunit (CTb: List Biological Laboratories, Campbell CA, Cat# 104) was reconstituted in 0.1M potassium phosphate buffer (1%, pH 6.0), and either pressure injected or delivered with current into primary visual cortex. …

Tissue fixation and histological processing:

…The sections were rinsed in PBS, then incubated for 48 hours in a solution containing a 1:5,000 dilution of anti-choleragenoid (primary antibody, List Biological Laboratories, Campbell CA, Cat# 703, RRID: AB_10013220), 2% NRS, 2.5% BSA, and 1% Triton-X. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)