Bacterial Toxin Research Citations

EAE:

…containing 500 µg of the heat-killed H37Ra strain of Mycobacterium tuberculosis (Difco, Detroit, USA). 200 ng of pertussis toxin (List Biological Laboratories, CA, USA) in 100 µl PBS was administered iv immediately after immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Ovalbumin food allergy model:

The OVA food allergy model has been described previously( 16 , 24 ). Briefly, 6-week-old conventional female BALB/c OlaHsd mice (10–15 animals per group) (Harlan Laboratories) were sensitised via the oral route daily during the first 3 d and then at weekly intervals with 20 mg of OVA (Sigma) along with 10 μg/mouse of cholera toxin used as an adjuvant (List Biological Laboratories) for a continuous period of 7 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Analysis of T-cell and monocyte population activation:

…Monocyte populations in the PBMC samples (200 l per well) were stimulated in separate ninety-six-well plates with 10 g/l of ultra-pure lipopolysaccharide (LPS; List Biological Laboratories, Inc.) per well or an equal volume of sterile endotoxin-free water as a negative control. PBMC were incubated at 37°C in 5 % CO₂ and the supernatants were removed after 24, 48 and 72 h for the analysis of monocyte-derived cytokines as described below.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Memory B Cell ELISPOT:

… Cultured PBMCs were washed then plated at 1:2 serial dilutions (3.75 10⁵ 1.6 10⁴ cells/well) in triplicate onto ELISPOT plates coated with 10 g/mL PA (List Biologicals, Campbell, CA, USA) … The frequency of PA-specific ASCs was defined as: [(anti-PA IgG spots per 100,000 input cells)/(total IgG spots per 100,000 input cells)] 100.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Cell Treatment:  

The cells were incubated in HDMEM + 10% FBS for 72 hours before they were
treated with either media (control), 1M CP 55,940 (Tocris; Bristol, UK), 100pg/mL,
1ng/mL, 10ng/mL, or 20ng/mL E. coli O55:B5 LPS (List Biological Laboratories, Inc.;
Campbell, CA),

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents:  the receptorbinding protein protective antigen and metalloprotease lethal factor were from List Biological

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

SNAPtide Assay[44]

Recombinant 425aa BoNT/A LC was used throughout all assays and was prepared as previously described.[45] All SNAPtide assays were run in 40 mM HEPES + 0.1% Triton X-100 at pH 7.4. BoNT/A LC concentrations were 18.5 nM while SNAPtide (prod. no. 521, List Labs) concentrations were 5 M. Fluorescence was recorded continuously for 1.5 h to calculate an accurate k_obs using equation 1. In the preincubation experiments, inhibitors at the specified concentrations were incubated with 925 nM enzyme and diluted 50-fold into substrate and initial rates of SNAPtide cleavage were measured. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

EAE:

…0.2 ml inoculum containing 200 µg MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 µg Mycobacterium tuberculosus hominis H37Ra (MT) on day 0 and 7 following [23], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and IVIg treatment:

Mice (8-10 wks of age) were immunized by subcutaneous injection of mice at two sites in the chest (50 µl/side) with an emulsion containing myelin oligodendrocyte glycoprotein amino acids 3555 (MOG₃₅₅₅) (2 mg/ml) (Stanford University Pan Facility, Stanford, CA) with Complete Freunds Adjuvant (CFA) containing heat killed Mycobacterium tuberculosis H37Ra (4 mg/ml; Difco, Detroit, MI) [29]. Mice were also injected i.p. with 75 ng of pertussis toxin (List Biologicals, Campbell, CA) on the day of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…emulsified 1:1 in Complete Freunds Adjuvant (CFA) to each hind flank (50 µL each), in addition to intravenous Pertussis toxin injection (300 ng in endotoxin-free PBS) (List Biological Laboratories, Campbell, CA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)