Bacterial Toxin Research Citations

EAE induction and treatment:

…Chronic EAE was induced in the female C57Bl/6 mice by subcutaneous immunization with MOG₃₅₅₅ (Anaspec, San Diego, CA), which was emulsified in Complete Freund’s adjuvant with 8 mg/ml of Mycobacterium tuberculosis H35RA (Difco, Detroit, MI) in 11 ratio, and 100 µl (100 ng) of emulsified MOG₃₅₅₅ was injected sub-cutaneously at each side of the base of the tail respectively. Each mouse also received 100 µl (200 ng) of pertussis toxin (List Biological Laboratories, Campbell, CA) by intraperitoneal (i.p.) injection, and the injection of toxin was repeated 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 Construction of the 3D skin:

… 1 nM cholera toxin (List biological laboratories, Inc., Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell Culture

… 100 ng/ml cholera toxin (List Biological Laboratories, Inc.), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materialien und Durchfhrung:

Pertussis toxin List Biological Laboratories, Campbell, US …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) model:

C57Bl/6 non-transgenic and shCD5ETg mice were immunized with a single s.c. injection of 150 µg of MOG₃₅₅₅ peptide (BioSynthesis, USA) emulsified in CFA (Difco, supplemented with 400 µg/mL of Mycobacterium tuberculosis) on day 0, followed by one i.p. injection of 200 ng pertussis toxin (LIST Biologicals, USA) on day 0 and another on day 2…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

VGLUT1 contacts on vertical cells in rats that had received sciatic nerve injections:

…The slices from these rats were initially incubated in avidin conjugated to Alexa 488 (Life Technologies; 1:500). They were then cut into 60 m thick sections with a vibrating microtome, and these were scanned to reveal the morphology of the recorded cells. Three of these were classified as vertical cells, and sections that contained most of the dendritic trees of these cells were incubated for 3 days in a cocktail consisting of guinea pig or rabbit antibody against VGLUT1 (Millipore; 1:5,000 and Synaptic Systems, Gttingen, Germany; 1:5,000, respectively) and goat anti-CTb (List Biological Laboratories, Campbell, CA, USA; 1:500). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Retrograde labeling of ascending projection neurons in the brainstem:

After 2 d of survival, mice were transcardially perfused and transverse brainstem sections (20 m thickness) were prepared using a freezing microtome. For fluorescent localization, sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X containing 0.12% -carrageenan, 1% NRS, and 0.02% sodium azide (PBS-XCR) for 30 min, 1:10,000 goat anti-CTB antibody in PBS-XCR (703; List Biological Laboratories…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… CT ( Choleratoxin ) List Biological Laboratories, Inc. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Intracellular detection of LFn fusion proteins and PARP cleavage:

… Detection of toxin proteins was achieved with rabbit anti-LF and mouse anti-CdtB, both made in our laboratory, and goat anti-Pseudomonas exotoxin A (List Biological Laboratories, Campbell, CA, USA). …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

Induction and Clinical Assessment of EAE:

…Mice were then immediately injected intravenously with 350 ng of pertussis vaccine (List Biological Laboratories, Campbell, USA) and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)