Citations

EAE:

C57BL/6 mice received 200 ng per mouse pertussis toxin (List Biological Laboratories Inc.) i.p. on the day of immunization and 2 d thereafter.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological Treatments:

To ablate AgRP neurons in adult mice, we administered two i.m. injections of DT (50 mg/kg; List Biological Laboratories) 2 d apart to 6-wk-old mice…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Administration of CT:

Mice were i.g. inoculated under anesthesia (as described above) with 10 µg CT (List Biological Laboratories, Campbell, CA) in a volume of 50 µl of 8.5% NaHCO₃ 16 to 24 h prior to infection. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Toxin challenge:

An in vivo oral CT challenge test was used as described previously (Tokuhara et al., 2010). After being fasted for 12 h, mice (10 per group) were orally challenged with 20 µg of CT (List Biological Laboratories, Campbell, CA)….

GM1-binding inhibition assay:

A GM1-binding inhibition assay was performed using a GM1-ELISA as described previously, with some modifications…The serum of macaques (10%, v/v) was treated with CT at a final concentration of 50 ng/mL for 1 h at RT and then incubated in 96-well plates…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

…LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA, USA). …

Results:

BCR stimulation elicits transcriptional and post-transcriptional activation of IB-:

Induction of IB- mRNA in LPS-stimulated macrophages requires NF-B-mediated transcriptional activation of IB- (10, 11). We, therefore, analyzed activation of the IB- promoter by reporter analyses. Upon stimulation of A20 cells with F(ab)₂ of anti-immunoglobulin antibody, a reporter harboring an IB- promoter fragment from 1725 to +115bp was significantly activated similar to the NF-B reporter pELAM1-Luc (Fig. 2A). A further truncated IB- promoter from 786bp was also activated in response to BCR engagement (Fig. 2B). This promoter region contains three NF-B binding sites, B1, B2 and B3. Introduction of mutations at the B2 site severely impaired activation in response to BCR engagement as in LPS-stimulated macrophages (11), indicating a critical role for NF-B in the transcriptional activation. Mutations at the B1 site moderately reduced activation, but mutations at the B3 site, whose sequence is not conserved between mouse and human, did not affect activation.

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…An intraperitoneal injection of 300 ng pertussis toxin (List Biological Labs, Campbell, CA, USA) was administered on the day of induction and 48 h later in EAE immunized mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…200 ng of pertussis toxin (List Biological Lab) was given ip on days 0 and 2 post immunization (pi). Mice were scored daily for appearance of clinical signs of EAE by a scale from 0 to 5 as described previously [18]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… Mice (48 weeks old) were immunized with 200 µg of myelin oligodendrocyte glycoprotein peptide (3555) (MOG) emulsified with Complete Freuds Adjuvant… Pertussis toxin (PTX), 250 ng (List Biologicals Laboratories, Campbell, CA), was injected intravenously on day 0 and again on day 2…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peptide synthesis and EAE induction:

…Mice were injected s.c. in their anks with 0.1 ml of the peptide emulsion (for a total of 100 mg MOG3555 and 400 mg M. tuberculosis/mouse) and, on the same day and 48 h later, were injected i.v. with 0.2 ml containing 200 ng Bordetella pertussis toxin (List Laboratories) dissolved in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Forty nanolitres of 1% low-salt cholera toxin B subunit (CTb) (, USA) was injected over a period of 1 min, and the needle was left in place for 10 min prior to removal, to allow for diffusion and to reduce spread up the injection tract. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt