Bacterial Toxin Research Citations

Reagents and cell culture:

Staphylococcus aureus enterotoxin B (SEB) was obtained from List Biological Laboratories INC. (Campbell, CA, USA) …

Anatomical tracer injections:

…The injection micropipette (tip ca. 15 m) was filled with cholera toxin subunit b (CTb; 1%, List Biological, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture and transfection:

DMEM/Hams F12 medium supplemented with… and 100 ng/ml cholera toxin (List Biological Labs). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Screening Against LF:

… Each well contained a volume of 90 L including buffer (20 mM HEPES, pH 7.4), recombinant LF (10 nM final concentration, List Biological Laboratories), and the fragment solution (1 mM final concentration). After a 20 min incubation period at 25 C, the reaction was initiated by the addition of 10 L fluorogenic LF substrate (2 M final concentration, MAPKKide DABCYL/FITC, List Biological Laboratories).

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

EAE Induction:

… Mycobacterium tuberculosis (MTB) H37Ra (Difco). At the time of EAE induction and 48 h later, mice were injected intraperitoneally with 200 ng pertussis toxin (List Biologicals) in 200 µL of PBS. Following the encephalitogenic challenge …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Injection of retrograde tracer:

CTb (2%, List Biological Laboratories, Campbell, CA, USA), dissolved in distilled water, was targeted into the right dlPAG (coordinates: 6.36.6 mm posterior to Bregma, 1.6 mm lateral to the midline, 4.44.9 mm from the surface of the dura mater, angle 15) of 13 animals. The tracer was delivered into the targeted area through a glass micropipette, either by pressure injection (pipette tip diameter 2030 m; volume ranged between 20 and 75 nl) using an automated air-pressure…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Induction:

Chronic progressive EAE was induced in 8-10 week old mice on day 0 by subcutaneous injection of a homogenized mixture of 200 µg MOG 35 55 peptide…An intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories, Campbell CA) in 100 µL of PBS was also delivered on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

As positive controls, cells were stimulated for 12 h with 10 ng/mL PMA and 500 ng/mL ionomycin or 100 ng/mL LPS (Salmonella Minnesota , List Laboratories). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

EAE:

…Immediately following MOG peptide injection and 24 h later mice were intraperitoneally (ip) administered 200 µg pertussis toxin (List Biological Labs, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and clinical assessment of EAE:

C57BL/6J Jcl (B6) female mice (n = 5-6 per group, 7-8 weeks old) were immunized subcutaneously at the base of the tail with 100 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) peptide (amino acid sequence, MEVGWYRSPFSRVVHYRNGK, derived from mouse MOG) dissolved in 0.1 ml phosphate buffered saline (PBS) and 0.1 ml CFA containing 1 mg of Mycobacterium tuberculosis strain H37Ra (Mtb H37Ra). Shortly after immunization and 48 h later, the mice were injected i.p. with 200 ng of Bordetella pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)