Bacterial Toxin Research Citations

Experimental Autoimmune Encephalomyelitis induction:
 
2.2.2 Immunization of mice
 
Mice were immunized by a subcutaneous injection of 100µl of the prepared emulsion in
the ventral thorax, with an injection of 50µl in two sides in the chest (left and right side).
At the time of immunization and 2 days after, mice were injected intraperitoneally with
75ng/ml pertussis toxin (List Biologicals, Campbell, CA). 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of MOG 35 – 55 immunization and two days later. The remaining six sham mice underwent the same immunization procedure with only IFA without MOG 35 – 55 . Mice were assessed daily for neurological disabilities using a standard clinical score [ 33 ] system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and methods – Reagents:

… LPS was purchased from List Biological Lab (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

ELISAs for the avidity measurement of anti-DFS70 autoantibodies and anti-diphtheria toxoid antibodies:

The ELISAs were performed as previously described [13-16], with modifications. Microtiter wells (Medisorp; Nunc, Roskilde, Denmark) were coated with recombinant DFS70 [9] or diphtheria toxoid (DT; List Biological Laboratories, Inc., Campbell, CA) in PBS (1 and 2 g/ ml, respectively). …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

Retrograde labelling of the neurons projecting to the rostral raphe pallidus:

CTb in the hypothalamus was detected with goat anti-CTb antiserum (1:1000, List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Chemicals and reagents:

Chemicals and reagents were purchased from the following sources: …endotoxin-free PBS and phenol-extracted LPS (E. coli O111:B4) that is free of lipoprotein contamination (List Biological Laboratories); …

Cell isolation:

…Platelet activation was induced for the stated time at 37C with 100 ng/ml LPS with addition of 100 ng/ml each of human rCD14 and recombinant LPS binding protein (LPB).

Mouse thrombosis:

…Whole blood of mice, injected or not with actinomycin D, was isolated 90 min after injection and stimulated with LPS for 60 min before leukocytes were isolated, and their ability to synthesize IL-1b mRNA relative to constitutive GAPDH was assessed by RT-PCR.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… Pertussis toxin (List Biological Laboratories, Campbell, CA, USA; 200 ng ip) was given on days 0 and 2. Animals were assessed daily in a blinded fashion using a 10 point scale score (EAE disease score) and sacrificed and assessed electrophysiologically as soon as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… The emulsion was administered sc as four 50µl injections into the flanks of each leg. In addition, 200 ng pertussis toxin (List Biological Laboratories) dissolved in 200 µl PBS was injected ip on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tract tracing and immunofluorescence:

Retrograde tract tracing was performed using a 1% solution of cholera toxin B subunit (CTB; List Biological Labs), injected using standard stereotaxic coordinates…Large areal injections of CTB were performed by pressure injection using a 33 ga stainless steel needle,…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Induction:

For the induction of EAE, C57BL/6 mice received a single immunization of MOG35-55 in Freunds complete adjuvant containing killed Mycobacterium tuberculosis H37Ra subcutaneously on day 0, followed by intravenous injection with 200 ng of pertussis toxin (List Biological Laboratories) on day 0 and 2 [39]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)