Bacterial Toxin Research Citations

Experimental protocols:

For each experiment, mice received 1 mg/kg ultrapure LPS from E. coli O111:B4 (List Biological Laboratory) administered intravenously (iv) 4 h prior to intravital microscopy. This concentration is sufficiently low that all animals survived general anesthesia, and perfusion in the liver vasculature remained such that leukocyte-endothelial interactions occurred under flow conditions. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… LPS (Salmonella minnesota R595) was from List Biological Laboratories. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Salmonella typhimurium lipopolysaccharide (LPS, List Biologicals Laboratories, Inc., Campbell, CA) at 1 g/mL, …

• Product #225 – LPS from Salmonella typhimurium

Materials and Methods –  Reagents:

… Purified LPS purchased from List Biologicals (San Jose, CA, USA) was dissolved in endo-toxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE induction:

At 9 weeks of age C57Bl/6 mice received a subcutaneous injection of 125g MOG35-55 peptide (Mimotopes, Australia) emulsified 1 : 1 (vol/vol) in Complete Freunds Adjuvant containing 4 mg/ml Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI), to both flanks and the base of the tail. Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Laboratories, Cambell, CA) treatment of macrophages was carried out as described (Holian, 1986). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… 200 µg myelin oligodendrocyte glycoprotein (MOG) 35-55 peptide (Biosynthesis) emulsified with CFA (final concentration of 300 µg Mycobacterium tuberculosis; Difco) and intraperitoneal pertussis toxin (500ng; List Biological Laboratories, Inc.) injections on day 0 and 2 (24). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… EAE was induced by injecting the mice subcutaneously into the flanks with 200 µl of emulsion containing 200 g MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) and 500 µg M. tuberculosis H37 Ra (Difco) in incomplete Freund Adjuvant oil (Difco). In addition, the mice received 400 ng pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Active CD4+ T cellmediated EAE was induce d by immunizing mice with 100 µg of rMOG emulsifie d in complete Freunds adjuvant (CFA) containing 1 mg/ml mycobacteria (Sigma) accompanied by two injections of 200 ng pertussis toxin (List Biological Laboratories) as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vitro migration and actin polymerization:

Migration assays were performed using 24-well transwell plates with 5 m pore size filter inserts (Corning). Prior to migration assays, L1.2 or THP-1 cells were pretreated with either 100 M BX-471 (kind gift of Richard Horuk, UC Davis, Davis, CA), 0.2 µg/mL pertussis toxin (List Biological Laboratories) or DMSO as a control for 1 h at 37°C. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)