Citations

… Lyophilized PTX (List Biological Laboratories Ltd, Campbell, CA) was purchased from Sapphire Biosciences, New South Wales, Australia. All chemicals used for experiments were of molecular biology grade and used as received unless otherwise specified. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Analysis of N-glycans attached to transgenic N. benthamiana-expressed CTB:

… After electrophoresis, proteins were transferred to a poly(vinylidene difluoride) membrane. Blots were probed with goat anti-CTB antiserum (List Biological Laboratories) …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and disease induction:

EAE was induced as previously described please (21) by injecting 0.2 ml of emulsion containing 125 g of MOG ₃₅₅₅ peptide… In addition, the mice received 300 ng of pertussis toxin (List Biological Industries, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CFA/I and LT Antigens:

The CFA/I fimbriae were isolated as previously described (Byrd et al., 2003) and the LT was obtained from List Biological Laboratories (Campbell, CA).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Botulinum neurotoxin type A was purchased from List Biological Laboratories, Inc. and used after reconstitution in PBS.

Immunohistochemistry:

Free-floating brainstem slices were incubated with antibodies on a shaking platform at room temperature. After each incubation period, sections were rinsed in TPBS 3  20 min. We used goat–CTB (1:100,000; List Biologicals, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Murine models of ALI:

…Female C57BL/6 mice, age 8 12 wk, were treated with 20 g LPS (Escherichia coli 0111:B4; List Biological Laboratories) in 50 l saline or 50 l saline intratracheally (i.t.) and euthanized 2 8 days later. …

• Product #201 – LPS from Escherichia coli O111:B4

Peptide synthesis and immunization procedures:

… To measure recall responses, MOG 3555 emulsified in complete Freund’s adjuvant (CFA) was administered s.c., in the inguinal and sternal regions (100 g per mouse). Disease was induced with peptide emulsified in CFA supplemented with Mycobacterium tuberculosis H37RA extract (Difco Laboratories, Detroit, MI) to a final concentration of 5 mg/ml (200 g/mouse). Additionally, pertussis toxin (PT; List Biological Laboratories, Campbell, CA) was administered i.p., (200 ng/mouse) on day 0 and day 2 postimmunization (Massilamany et al., 2011c).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (List Biological Laboratories): 1 mg/ml dissolved in 18.2 M H 2 O, stored at 20°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cholera toxin immunisation:

TCR^/ mice were sublethally irradiated (500 rad) and reconstituted with B6 or Rorc^/ bone morrow. Before immunization with cholera toxin mice were deprived of food for 2 h and then given 0.25 ml of a solution containing eight parts HBSS and two parts 7.5% sodium bicarbonate by oral gavage (o.g.) to neutralize stomach acidity. After 30 min, mice were o.g. immunized with 25 µg cholera toxin (List Biological Laboratories, USA). Concentrations of antibodies specific for cholera toxin were determined by ELISA with cholera toxin (List Biological Laboratories) as the capture agent.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae