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4165 citations found

Sensitive and Specific Assay to Measure Endoprotease Activity of Botulinum Toxin Type A Holotoxin in Milk.

Shine, N.; Eaton, L.; Crawford, K.

Product: Botulinum Neurotoxin Type A from Clostridium botulinum

Correction of Murine Hemophilia a by Hematopoietic Stem Cell Gene Therapy

Moayeri M, Hawley TS, Hawley RG

Product: Tetanus Toxoid from Clostridium tetani

  • FVIII and Tetanus Toxoid Immunizations:

    ... Two of the 800 cGy-irradiated mice (mice #26 and #27) and four naive E16 mice were immunized with tetanus toxoid (List Biological Laboratories, Inc., Campbell, CA, USA) 3 weeks after the final FVIII injection as follows: 4 g tetanus toxoid was mixed with 1 mg Al (OH)3adjuvant (Alhydrogel 2%; Accurate Chemical & Scientific Corporation, Westbury, NY, USA), diluted in sterile saline to a total volume of 108 l and injected intraperitoneally. Two weeks later, sera from these mice was assessed for the presence of anti-tetanus toxoid antibodies by ELISA (described below).

Anthrax Lethal Toxin-Mediated Killing of Human and Murine Dendritic Cells Impairs the Adaptive Immune Response

Alileche A, Serfass ER, Muehlbauer SM, Porcelli SA, Brojatsch J

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

  • Materials and Methods:

    ... Recombinant anthrax LF was obtained from List Biological Laboratories (Campbell, California, United States). PA was generously provided by Dr. Steven Leppla (National Institutes of Health). PA and LF were reconstituted in water at 500 ng/ml and 250 ng/ml, respectively [35]. Recombinant LF and PA were produced in B. anthracis and were free of LPS contamination as indicated by the manufacturer, and by a lack of CD86 up-regulation on immature cells, as measured by flow cytometry.

    Cell death and viability assays:

    Cell viability was measured by analysis of MTT cleavage to formazan by succinate dehydrogenases in living cells [37]. For the colorimetric MTT assay, cells were exposed to LT (500 ng/ml PA and 250 ng/ml LF), and the MTT solution (5 mg/ml MTT in PBS) was added directly to wells and incubated at 37 C for 4 h. The dye was solubilized with acidic isopropanol (25 mM HCl and 0.5% SDS in isopropanol), and the absorbance was measured at 570 nm. ...

    Western blotting:

    Cells were cultured in 24-well plates and treated with LT.  ...

    In vivo assay:

    Ten-week-old female BALB/c mice were injected intraperitoneally with LT [3,4]. Mice were sacrificed 0, 3, 6, and 24 h after LT injection. Spleens were harvested and treated with 400 U/ml collagenase D (Roche) to release DCs. Levels of splenic DCs (CD11c+, MHC class II+), macrophages (CD11b+, MHC class II+), and circulating B cells (B220+) and T cells (CD3+) were determined by flow cytometry using fluorescently labeled antibodies to surface markers.


    LT (Lethal Toxin) = Lethal Factor (LF) + Protective Antigen (PA)

    Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Physical Characteristics of rLF and rPA: Effects on Enzymatic : Effects on Enzymatic Activity and Binding

Shine, N., Le, C., Eaton, L., Crawford, K.

Product: Unquenched Calibration Peptide for MAPKKide® 530 for Anthrax Lethal Factor

A Soluble Receptor Decoy Protects Rats against Anthrax Lethal Toxin Challenge

Scobie HM, Thomas D, Marlett JM, Destito G, Wigelsworth DJ, Collier RJ, Young JA, Manchester M

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Efficient synthetic inhibitors of anthrax lethal factor

Forino M, Johnson S, Wong TY, Rozanov DV, Savinov AY, Li W, Fattorusso R, Becattini B, Orry AJ, Jung D, Abagyan RA, Smith JW, Alibek K, Liddington RC, Strongin AY, Pellecchia M

Product: MAPKKide® Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor

Comparison of Activity of Botulinum Neurotoxin Type A Holotoxin and Light Chain Using SNAPtide FRET Substrates.

Christian, T.; Shine, N.; Eaton, L.; Crawford, K.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

The Chinese herbal medicine formula FAHF-2 completely blocks anaphylactic reactions in a murine model of peanut allergy

Srivastava KD, Kattan JD, Zou ZM, Li JH, Zhang L, Wallenstein S, Goldfarb J, Sampson HA, Li XM

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Mice and reagents:

    Five-week-old female C3H/HeJ mice purchased from the Jackson Laboratory (Bar Harbor, Me) were maintained on peanut-free chow under specific pathogen-free conditions according to standard guidelines for the care and use of animals …. Reagents were purchased from the following sources: cholera toxin, List Biological Laboratories, Campbell, Calif; …

    Intragastric peanut sensitization, challenge, and FAHF-2 treatment:

    Mice were sensitized intragastrically with ground peanut (10 mg/ mouse) and cholera toxin (20 mg/mouse) weekly for 3 weeks as previously described,14 then treated intragastrically with FAHF-2 (20 mg/mouse in 0.5 mL water) twice daily for 7 weeks (weeks 3-9). …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Determination of serum IgG antibodies to Bacillus anthracis protective antigen in environmental sampling workers using a fluorescent covalent microsphere immunoassay

Biagini RE, Sammons DL, Smith JP, Page EH, Snawder JE, Striley CA, MacKenzie BA

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

  • Antigen:

    Recombinant PA, produced in an avirulent, non-capsulated, sporulation suppressed Ba host was obtained from List Biological Laboratories, Inc. (Campbell, CA). The PA migrated as a single major band with an apparent molecular weight of 83 000 daltons on 10% polyacrylamide gels in the presence of sodium dodecyl sulphate (SDS). The PA was reconstituted in distilled water and stored aliquoted at 220C. Before use, individual aliquots were thawed and used immediately.

    Competitive inhibition:

    To determine the specificity of measurements performed by FCMIA, pre-incubation of positive sera, control sera, negative worker sera, and a dilution of AVR414 with PA (competitive inhibition) was performed. Sera (130 ml final volume) were treated with either 80 mg/ml PA (final concentration) or dilution buffer and incubated overnight at 4C. The sera were then centrifuged and the supernatants analysed as outlined above. ...

Expression and Immunogenicity of a Recombinant Diphtheria Toxin Fragment A in Streptococcus gordonii

Lee CW, Lee SF, Halperin SA

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • Intranasal immunization:

    BALB/c mice (n = 5) were intranasally immunized with 109 live S. gordonii DTA2 cells in 25 µl of PBS containing 10 µg of cholera toxin subunit B (CTB; List Biological Laboratories, Inc., Campbell, Calif.) as the mucosal adjuvant. The method of immunization has been described elsewhere (20, 21). A second group of mice received 10 µg of CTB alone as a control. The animals received additional immunizations on days 21, 33, 47, and 54. The animals were euthanized on day 70, and serum samples and saliva and vaginal wash samples were obtained as described previously (10, 20).

    DT neutralization assay:

    The DT neutralization assay was performed as described by Miyamura et al. (26) with modifications. Briefly, sera in triplicate were diluted in serial twofold dilutions (starting dilution at 1:2) with growth medium (minimal essential medium with 1% L-glutamine; Invitrogen Life Technologies, Burlington, Ontario, Canada) supplemented with 10% fetal bovine serum, 0.01% amphotericin B, 0.3% gentamicin, and 0.2% penicillin G. The diluted samples (12.5 µl) were then transferred to 96-well flat-bottomed Nunclon microplates (VWR International Ltd., Toronto, Canada), and an equal volume of native DT (0.8 ng/ml; List Biological Laboratories) was added. The mixtures were incubated at 37°C for 1.5 h, after which 50 µl of Vero cell suspension (1.2 105 cells/ml) and 150 µl of growth medium were added to each of the wells. The cultures were then incubated in a 5% CO2 incubator for 5 to 7 days at 37°C and scored with the aid of an inverted microscope for toxicity: +, confluent monolayer; , approximately 50% cell death; , 100% cell death. Neutralization titer was defined as the reciprocal of the dilution that showed complete neutralization (+, confluent monolayer) of DT cytotoxicity.

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae