Bacterial Toxin Research Citations

Retrograde and Transganglionic Labeling using Cholera Toxin b Subunit:

Cholera toxin b subunit (CTb) was used to retrogradely label the motor pools of selected forelimb muscles and to anterogradely (i.e., transganglionically) label muscle afferents as a means to help localize CST terminations. CTb preferentially labels myelinated afferents [18], [19]. Studies have shown a correspondence between CTb-labeled proprioceptive afferents and intra-axonally labeled group 1 afferents [20] and group 1 field potentials [21]. We performed multiple injections of CTb (11000, List Laboratories) unilaterally into the deltoid, elbow flexor, and the wrist extensor compartments (42.5 µL; 33 ga. Hamilton syringe). Our intent was to label selectively muscles that act at the same joint. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Assay for LPS-Stimulated Human Whole Blood:

Whole blood from seventy healthy volunteers was collected into EDTAVacutainertubes.Assayswereperformedafterdiluting the blood samples 9:1 in RPMI-1640 media supplemented with glutamine and antibiotics. Aliquots of 2mL of diluted blood per well were equally divided into LPS stimulated (100ng/mL of E. coli LCD25; List Biological Laboratories, Campbell, CA, USA) …

• Product #314 – LPS from Escherichia coli K12 strain LCD25

Materials:

…CT …were purchased from List Biologicals (Campbell, CA). …

Assay for Holotoxin Disassembly:

CT was appended to a GM1-coated SPR sensor slide as previously described [18]. PDI (0.1 mM) was then perfused over the CT-coated sensor in PBS containing 0.05% Tween 20, 1 mM GSH, and either grape seed or grape pomace extract. …

CTA1 Translocation and Secretion Assays:

HeLa cells exposed to GM1 and CT were processed as described above for the toxin transport assay, except (i) media samples were collected before lifting the cells from the plates; (ii) the cell pellet was resuspended in 1 mL HCN/digitonin buffer; and (iii) the cytosolic fraction was collected and diluted to a final volume of 1 mL in HCN buffer. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and treatment:

…cholera toxin (10 ng/ml; List Biologicals, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experimental Protocol:

Autoimmune cholangitis was induced as previously described …injected intraperitoneally (I.P.) into 8 week-old female B6 mice. The mice were subsequently boosted 2 weeks later with 2OA-BSA in incomplete Freunds adjuvant (IFA, Sigma-Aldrich). Additionally, mice received 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) on the same day and 2 days following the initial …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… They were also injected with 500 ng of pertussis toxin (List Biological Laboratories, Inc, Campbell, CA) intraperitoneally on Day 0 and 2. 20 µg of cholera toxin (List Biological Laboratories, Inc, Campbell, CA)…100 ng/ml of LPS (List Biological Laboratories) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Bordetella pertussis toxin (200 ng, PTX, List Biological Laboratories, Campbell, CA) was administered intra-peritoneally at days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

C57BL/6, trkB+/ and trkB+/+ (wt littermates) mice (5 generations of backcrossing, JAX stock 002544) were immunized subcutaneously on day zero in four sites over the back (left and right shoulder, left and right flank) with 200 g of MOG₃₅₅₅ (MEVGWYRSPFSRVVHLYRNGK) (Princeton Biomolecules, Langhorne, PA) emulsified in a volume of 100 l of complete Freunds adjuvant (CFA) containing 1 mg/ml of M. tuberculosis (M. Tbc) (CFA, Difco Laboratories, Detroit, MI, USA) (ONeill et al., 2006; Papenfuss et al., 2004). On days 0 and 2 post-immunization, 200 ng of pertussis toxin (PT) (List Biological, Campbell, CA) was administered intraperitoneally (i.p.) in 0.2 ml of PBS …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

IMC isolation and differentiation:

B6 mice were immunized with an emulsion of 100 ml PBS and 100 ml CFA… Each animal also received 200 ng pertussis toxin (PT; List Biological Laboratories) i.v. on days 0 and 2 postimmunization. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental protocol:

…To examine the role of chemoattractant receptors and CCR4 on Treg migration during CS, mice underwent sensitization and challenge as already described. Subsequently, anesthetized mice were injected intradermally with either PTx (List Biological Laboratories, Campbell, CA; 100 ng in PBS)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)