Bacterial Toxin Research Citations

EAE induction and TFA-12 treatment:

Mice were given intravenous injections of pertussis toxin (List Biological Laboratories) on the day of the immunization and 48 h later and were divided in two experimental groups.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune encephalomyelitis (EAE):

EAE was induced in C57BL/6 wild type, Panx1^+/+ and Panx1^/ mice by subcutaneous immunization with 300g of myelin oligodendrocyte glycoprotein MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK;… The day of MOG immunization was designated day 0. On day 0 and day 2 post immunization (dpi), mice were injected intraperitoneally with 500ng Pertussis toxin (List Biological Laboratories, Campbell, CA, USA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cholera toxin B subunit (CTB) tracer injection:

After 67 d, a CTB tracer was injected in the sciatic nerve. Anesthesia was induced in all rats with 5% isoflurane and maintained at 2.5%. The sciatic nerve of the left hindpaw was exposed, and each of the two branches was injected with 1 µl of CTB solution (#104; List Biological Laboratories; 10 mg/ml)…

Immunohistochemical labeling of CTB:

To examine the regeneration of the dorsal column fibers, animals were killed 10 weeks after the lesion and 3 d after CTB injection and CTB signal was visualized and quantified as follows…blocked with TBS containing 2% horse serum (Invitrogen) and 0.3% Triton X-100 (blocking buffer) for 45 min and incubated at 4C with goat-anti-CTB (#703, List Biological Laboratories; 1:80,000)

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Toxin, Antibodies, and Reagents:

… The anti-PA antibody was purchased from List Biological Laboratories, Inc (Campbell, CA, USA). …

Materials:

Recombinant PA, EF, and LF toxins were obtained from the List Biological Laboratories, Inc. … Stable toxin solutions of PA, EF, and LF were prepared according to the supplier’s technical note (List Biological Laboratories, Inc., Campbell, CA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis 

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Production and purification of rPA and rLF preparations:

MLA:

The J774A.1 mouse macrophage cell line (ATCC; TIB-67, Manassas, VA) was cultured in 75-cm² flasks in Dulbecco’s modified Eagle’s medium with high glucose … Culture medium was discarded before incubation with rPA and rLF. The macrophage lysis assay (MLA) using different concentrations of rPA and rLF was performed by diluting reagents in appropriate volumes of complete DMEM.

• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

… The Strep-tag of rCdtA was cleaved with enterokinase, followed by repurification of CdtA on Ni-NTA agarose to remove the cleaved tag and the enterokinase. Native toxins A and B were purchased from List Biological Laboratories. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

…  List Biological Laboratories, Campbell, California, USA. …

• Product #152C- Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Bacterial strains and reagents:

CT was purchased from List Biological Laboratories (Campbell, CA).

Histopathology:

Lungs were taken at various times after intranasal administration with V. cholerae O1 Inaba (10⁸CFU per mouse) or CT (0, 1, 3, or 10 µg per mouse). Individual lungs were fixed with formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… Escherichia coli O111 B4-derived LPS was from List Biological Laboratories, Inc. (Campbell, CA, USA).

Inhibition of E.coli LPS-induced COX2 gene expression by tocopherols:

The results are shown in Figure 3. Tocopherols significantly inhibited the expression of the COX2 gene in
RAW264.7 cells induced by LPS (p<0.01). …

• Product #201 – LPS from Escherichia coli O111:B4