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December 3, 2012
Frontiers In Cellular And Infection Microbiology
Burtnick, MN;Heiss, C;Schuler, AM;Azadi, P;Brett, PJ;
Product: Diphtheria Toxin CRM197, Mutant
Glycoconjugate Synthesis:
… To facilitate conjugation of the OPS antigens to cationized bovine serum albumin (cBSA; Pierce), diphtheria toxin mutant CRM197 (CRM197; List Biological Laboratories, Inc.) … the activated OPS samples were added to small amber vials. …
December 1, 2012
Thesis
Prashad, RC;
Product: Botulinum Neurotoxin Type B Light Chain, Recombinant
Vesicle-associated membrane protein (VAMP)-specific tetanus neurotoxin light-chain (TeNT-LC) and botulinum neurotoxins B and D light-chain (BoNT/B-LC and BoNT/D-LC, respectively) were purchased from List Biologicals Laboratories, Inc. (Campbell, CA., USA).
December 1, 2012
Aids Research And Human Retroviruses
Jones, RB;Kovacs, C;Chun, TW;Ostrowski, MA;
Product: Tetanus Toxoid from Clostridium tetani
Assessing levels of virus production upon antigenic stimulation:
Peripheral blood mononuclear cells (PBMCs) were cultured at 5106 cells/ml in stimulation medium [RPMI+10% human AB serum+1 g/ml anti-CD28+1 g/ml anti-CD49d+1 M zidovudine (AZT)+1 M lamivudine (3TC)+1 M nevirapine (NVP)] with either 1 g/ml cytochrome c (Austral Biologicals), 1 g/ml HIV-1-p55 (Austral Biologicals), 1 g/ml tetanus toxoid (List Biological Laboratories), …
December 1, 2012
Thesis
Cella, L;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Materials:
… PA toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …
Cloning:
In the ACE process the aptamers were initially labeled with a 6-carboxyfluorescein and incubated with increasing concentration of PA Toxin. …
December 1, 2012
Toxins
Garman, L;Dumas, EK;Kurella, S;Hunt, JJ;Crowe, SR;Nguyen, ML;Cox, PM;James, JA;Farris, AD;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Recombinant Protein ELISAs:
Ninety-six well plates were coated overnight at 4 C with 1 g/well of rLF or rPA (List Biologicals, Campbell, CA). After washing with PBS-Tween and blocking with PBS/BSA, diluted pre-immune and Day 28 (4 days after a second booster) serum from each mouse was added in duplicate and incubated for 2 h at room temperature (RT). …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
December 1, 2012
Infection And Immunity
Langer, M;Duggan, ES;Booth, JL;Patel, VI;Zander, RA;Silasi-Mansat, R;Ramani, V;Veres, TZ;Prenzler, F;Sewald, K;Williams, DM;Coggeshall, KM;Awasthi, S;Lupu, F;Burian, D;Ballard, JD;Braun, A;Metcalf, JP;
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Challenge with anthrax toxin or MEK pathway inhibitors:
At indicated time points, cultured cells were treated with fresh medium containing 2 g/ml PA or 2 g/ml LT (2 g/ml PA plus 2 g/ml LF), both from List Biological Laboratories, Inc., Campbell, CA. An equal volume of diluent was used as a negative control. Duration of treatment was 24, 72, or 144 h or as indicated. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
December 1, 2012
Book
Vanden Broeck, D;De Wolf, MJS;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Materials:
Highly purified CT was obtained from List Biological Laboratories (Campbell, Ca.). CT was radiolabeled with 125I using the Iodo-gen method as described by Fraker and Speck (1978). …
Preparation of membranes from post nuclear supernatant of a Vero cell homogenate:
Vero cells were grown to confluency in 175cm2 culture flasks and maintained in culture for at least 14 days before harvesting. … Cells were then labeled with 125I-CT (106cpm /2 ml 10nM) in the same medium containing 0.01 % bovine serum albumin for 30 min at 4°C to allow binding without endocytosis. …
Gradient centrifugation:
Subcellular fractionation was always performed on freshly prepared post nuclear membranes or PNS from cells labeled with 125I-CT. …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
December 1, 2012
Clinical And Vaccine Immunology
Schmied, J;Rupa, P;Garvie, S;Wilkie, B;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Treatments and sensitization with ovomucoid:
… Days of age were equivalent to experimental days. On days 14, 21, and 35, intraperitoneal (i.p.) injections of 100 g of purified Ovm, prepared in lab (33), adjuvanted with 10 µg of cholera toxin (List Biologicals, CA), were given to all pigs as previously described …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
December 1, 2012
Thesis
Blackwell, AE;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
… Cholera toxin (CTx) was purchased from List Biological Laboratories, Inc …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
December 1, 2012
Thesis
Mattsson, J;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Preparation of fusion proteins and CT-conjugates:
… CT-OVA and CTB-OVA conjugates were produced using the crosslinker N-succinimidyl 3(2-pyridyldithio) propionate, (SPDP). CT (LIST Biological Laboratories), rCTB (provided by Dr. J. Holmgren at the Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden) and OVA were coupled to SPDP and conjugated as described before [351]. Concentration of the conjugates, and the OVA content, was determined by ELISA against a standard dilution of CT, CTB and OVA. The molar ratio of OVA to CT or CTB was 4:1.
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae