Bacterial Toxin Research Citations

…Pertussis toxin (PTX) and Ly294002 were obtained from List Biological Laboratories (Campbell, CA) and Cayman Chemicals (Ann Arbor, MI), respectively. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Effects of I3C and DIM on EAE in mice:

… Following immunization, 200ng of pertussis toxin (List Labs) was injected i.p. into mice on Day 0, followed by a 400ng pertussis toxin i.p. injection on Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE and evaluation:

...Additionally, at day 0 and 2, mice were administered 250 ng of pertussis toxin (PTX, List Biological Laboratories, Campbell, CA) via intraperitoneal (i.p.) injection.

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 - Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 - Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

Active EAE and evaluation:

On day 0, B6 mice were s.c. immunized with 100 mg MOG3555 in CFA supplemented with… Additionally, at days 0 and 2, mice were administered 250 ng pertussis toxin (List Biological Laboratories, Campbell, CA) via i.p. injection. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active induction of EAE and clinical evaluation:

EAE was induced by immunizing male LIGHT+/+ and LIGHT2/2 mice s.c. over two sites on the anks with either 50 mg or 150mg myelin oligodendrocyte glycoprotein peptide (3555) (MOG3555) (MEVGWYRSPFSRVVHLYRNGK)… Pertussis toxin (PTx) (250 ng; List Biological, Campbell, CA) was injected i.v. at the time of immunization and repeated 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Lymphocyte homing assay:

…In additional experiments, CFSE-labeled lymphocytes (4 x 10⁷ cells per milliliter) were treated with 100 ng/ml pertussis toxin (PTX) (List Biological Laboratories)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune encephalomyelitis (EAE):

Mice were given 100 ng pertussis toxin (list Biological Laboratories) intraperitoneally on days 0 and 2. Mice were sacrificed 3 wk later. EAE was scored as follows: 0, no diseases; 1, limp tail; 2, hind limb weakness; 3, partial paralysis and hind limb paralysis; 4, front and hind limb paralysis; 5, death.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.8 Retrograde tracing and immunohistochemistry for CTb:

In order to identify potential circumventricular organ projections to the BLA, we used cholera toxin B-subunit (CTb) as a retrograde tracer to identify neurons with direct afferent projections to the BLA. Basolateral amygdala injection sites were taken from a previous experiment examining activation of an anxiety-related neuronal circuit projecting to the BLA and thus the procedure for iontophoretic injection of CTb and exposure to an open-field was conducted… Briefly, prior to CTb injection rats were anesthetized between 4 and 6 h after light onset with Hypnorm-Dormicum mixture…  A glass microelectrode was broken to a final tip diameter of 15-20 µm and a solution of dialyzed 4% CTb (Cat. No. 104, LIST Biological Laboratories, Campbell, CA) in PBS was applied iontophoretically using positive current pulses of 10 mA (7 s on; 7 s off) for 10 min. …

One set of slices was used for double immunostaining using primary antibodies directed against CTb (goat anti-CTb, Cat No. 703, 1:3000 List Biological Laboratories, Campbell, CA) and …

Immunohistochemistry for CTb and c-Fos was conducted on free-floating tissue in 30 ml tubes and gently shaken on an orbital shaker throughout double immunostaining. Tissue was rinsed in 3% hydrogen peroxide (H₂O₂) in PBS for 30 min, followed by washing in PBS containing 0.1% Triton X-100 (PBST) for 310 min; tissue was then blocked in 1% human serum albumin (HSA) in 0.1% PBST for 30 min. Sections were incubated overnight at 4° C with 1:3000 goat anti-CTb in 1% human serum albumin(HSA)/PBST. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Induction of EAE and Therapeutic Study:

In addition, 200 ng/100 L of pertussis toxin (List Biological Laboratories Inc.) was injected intraperitoneally on the day of immunization (day 0) and 2 days post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)