Bacterial Toxin Research Citations

EAE induction:

Mice were injected i.p. with pertussis toxin (400 ng/dose; List Biological Labs) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On day 0, each mouse was first given 200 ng pertussis toxin (List Biological Laboratories, Campbell, Calif., USA) in 200 µl PBS intraperitoneally (ip). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay (ELISA):

A 50 L of a 5 g/mL solution of activated PA63 (List Biological Labs, Campbell, CA, USA) dissolved in 50 mM carbonate-bicarbonate buffer (pH 9.6) (Sigma-Aldrich) was used to coat each well of a 96-well transparent flat plate …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Reagents:

…Recombinant forms of Bacillus anthracis PA63, LF, and EF were obtained from List Biological Laboratories (Campbell, CA) and reconstituted per the manufacturer’s instructions. …

The following applications were performed using the above listed Anthrax products:

• Integration of recombinant PA₆₃ artificial membranes
• The pH dependence of PA₆₃ conductance and LF binding
• Effect of EF/ LF present during acidification
• Planar membrane rupture by recombinant proteins
• Planar membrane rupture by cationic polymers
• Planar membrane rupture by animal isolates
• Droplet membrane rupture by animal isolates
• Molecular modeling

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

 Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

ELISA assays:

For stimulation experiments, cells were grown in 48 well plates for 4 days and the indicated stimulus was added in triplicates to the cell medium. E. coli Dm31 LPS was purchased from List Biological Laboratories (Denver, CO), …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

Endotoxemia:

Ultra Pure LPS (Escherichia coli 0111:B4) from LIST Biologicals (Campbell, CA) was dissolved in sterile normal saline and injected intraperitoneally (1.5 mg/kg). At various time points after LPS, mice were euthanized, blood was isolated by cardiac puncture, and the kidneys were harvested.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… For active EAE induction, mice were immunized in two sites on the hind flanks with 300 µg MOG35-55 in 100 µl CFA containing 5 mg/ml heat-killed M. tuberculosis strain H37Ra (Difco) at day 0 and day 7. Pertussis toxin (List Bio Lab) was administrated intraperitoneally at the dosage of 500 ng per mouse at day 1 and day 8. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

LPS from Escherichia coli O111:B4 was from List Biological Laboratories (Campbell, CA). …

In Vitro Generation and Activation of BMDCs:

BMDCs were generated from gal3^+/+ and gal3^/ mice by culturing bone marrow cells for 8 to 10 days as described… For measuring cytokine production, 2 10⁵ DCs per well were stimulated with 0.1 or 100 ng/mL of LPS; 1, 10, or 100 g/mL of curdlan; or 10⁵, 10⁶, 10⁷ cells/mL of HKCA in 96-well plates at 200 L per well for 24 hours.

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cell culture, construction of EST library, and screening of EST libraries with TcdB:

…To identify clones that are less sensitive to TcdB killing, cultures containing 2 10⁶ HeLa-tTA EST library cells or a similar number of HeLa-tTA naive cells were plated in a 150-mm culture dish and treated with 2 ng/ml TcdB (List Biological Laboratories, Inc., Campbell, CA). …

• Product #155 – Toxin B from Clostridium difficile