Bacterial Toxin Research Citations

Reagents:

Lethal Toxin, consisting of protective antigen and lethal factor, was from List Biological Laboratories.

…cells used for the Lethal Toxin stimulation were from BALB/c mice.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Mice and in vivo treatments:

DC depletion (in mice reconstituted with CD11c-DTR marrow) was accomplished by injection of diphtheria toxin (DT) (5 ng/g, IV; List Biological) 2 days in a row.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The right hindlimb was gently shaved and disinfected. A small incision was done in the skin above the tibialis ante- rior muscle, and 5 µl of 0.5% cholera toxin subunit B (CTB, List Biological Laboratories) were injected, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials and Cell Culture:

.. pertussis toxin by List Biological Laboratories. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CTb (list biological laboratories Inc., Campell, CA)

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Tetanus toxoid-specific human T-cell response:

For in vitro TT stimulation, dendritic cells were incubated with 2gml¹ TT (List Biological Laboratories) overnight.

• Product #190 – Tetanus Toxin from Clostridium tetani

Vaccine Preparation and Treatment Groups:

…The number of foals in each group was determined a priori, and foals were assigned randomly to each of the groups. The study groups were as follows: 1) low-dose inactivated virulent R. equi group (n=9), receiving 210¹⁰ CFUs of inactivated R. equi combined with 100 µg of cholera toxin subunit B (CTB, List Biological Laboratories, Campbell, CA) as a mucosal adjuvant, diluted in 100 ml of saline administered via nasogastric intubation; 2) high-dose inactivated virulent R. equi group (n=10), receiving 110¹¹ CFUs of inactivated R. equi with 100 µg of CTB diluted in 100 ml of saline via nasogastric intubation; 3) live virulent R. equi group (n=6), receiving 110¹⁰ CFUs of live R. equi diluted in 100 ml of saline administered via nasogastric intubation; 4) control with CTB group (n=9), receiving 100 µg of CTB diluted in 100 ml of saline via nasogastric intubation; and, 5) control without CTB group (n=8), receiving 100 ml of saline via nasogastric intubation. Treatments (i.e., live bacteria, inactivated bacteria, and negative controls) were administered by nasogastric intubation to foals at 2, 9, 16, and 23 days of age.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

… On the day of immunization (day 0) and 48 h later (day 2), PTx (List Biological laboratories Inc …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals:

Male Wistar rats … and male heterozygous transgenic VP-EGFP Wistar rats (56 weeks old) were used.

Retrograde Tracing:

…cholera toxin B (CTB) (1%; List Biological Laboratories) was microinjected into the RVLM (starting from bregma: 12 mm caudal along the lamina, 2 mm medial lateral, and 8 mm ventral). Animals were used 34 days after surgery.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Briefly, ninety-six well polystyrene plates (Immulon 4 HBX, Thermo Electron Corp., Milford, MA) were coated with 1 g of purified tetanus toxoid (List Biological Laboratories, USA) at a 20 g/mL concentration in Carbonate Bicarbonate Buffer (pH 9.6). …

• Product #191 – Tetanus Toxoid from Clostridium tetani