Bacterial Toxin Research Citations

Reagents were purchased as follows: edema factor (LIST Biologicals)

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Bacterial strains and chemicals:

… CT was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials, antibodies, and cell culture:

…BEAS-2B cells were grown in Ham’s F-12 medium (MediaTech) supplemented with 1 ml bovine hypothalamus extract (PromoCell), insulin (2 mg/ml) (Sigma), transferrin (2.5 mg/ml) (Sigma-Aldrich), dexamethasone (0.05 mM) (Sigma), cholera toxin (10 µg/ml) (List Biological Laboratories, Inc.), and epidermal growth factor (10 µg/ml) (Millipore). HBE cells were grown in modified Eagle’s medium supplemented with 10% FBS, 1% l-glutamine, and 0.01% gentamicin. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Protein expression and purification:

…Protein was detected by immunoblotting using commercial polyclonal goat anti-toxin A (List Biological Laboratories, Campbell, CA)…

Immunization Regimen:

…To perform a direct comparison to the formalin-inactivated toxoid vaccine currently in human clinical trials, we immunized one cohort of mice with the toxoid preparation by the i.p. route using alum as the adjuvant. Toxoid A and toxoid B from C. difficile…were purchased from List Biological Laboratories.

Mice, cells and reagents:

… Cells were exposed to Salmonella minnesota ultra pure lipopolysaccharide (LPS, List Biologicals Laboratories), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

…For lipid staining, cells were transferred to sterile coverslips in 24-well plates. Macrophages were activated with ultrapure LPS isolated from Salmonella minnesota (List Biological Laboratories, Campbell, CA, USA; …

• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

… LPS List Biological Lab (San Jose, CA, USA) …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Macrophage bacterial killing assay:

Isolated rat or human macrophages were exposed to 25 g/mL volcanic ash for 2 hr and primed with 10 ng/mL lipopolysaccharide (LPS; LIST Biological Laboratories, Campbell, CA, USA) for 1 hr. 

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Drugs and Chemicals. E. coli lipopolysaccharide (LPS) 0111:B4 was purchased from List Biological Laboratories Inc. …

Thermal Injury and LPS Administration:

… E. coli lipopolysaccharide (LPS) 0111:B4 was dissolved in normal saline at a final concentration of 0.2mg/mL. Doses of 0.1mg/kg and 0.3mg/kg body weight were injected into the jugular vein via the jugular cannula. …

• Product #201 – LPS from Escherichia coli O111:B4

 …Pertussis toxin was from List Biological (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)