Bacterial Toxin Research Citations

Reagents:

…LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA, USA). …

Results:

BCR stimulation elicits transcriptional and post-transcriptional activation of IB-:

Induction of IB- mRNA in LPS-stimulated macrophages requires NF-B-mediated transcriptional activation of IB- (10, 11). We, therefore, analyzed activation of the IB- promoter by reporter analyses. Upon stimulation of A20 cells with F(ab)₂ of anti-immunoglobulin antibody, a reporter harboring an IB- promoter fragment from 1725 to +115bp was significantly activated similar to the NF-B reporter pELAM1-Luc (Fig. 2A). A further truncated IB- promoter from 786bp was also activated in response to BCR engagement (Fig. 2B). This promoter region contains three NF-B binding sites, B1, B2 and B3. Introduction of mutations at the B2 site severely impaired activation in response to BCR engagement as in LPS-stimulated macrophages (11), indicating a critical role for NF-B in the transcriptional activation. Mutations at the B1 site moderately reduced activation, but mutations at the B3 site, whose sequence is not conserved between mouse and human, did not affect activation.

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…An intraperitoneal injection of 300 ng pertussis toxin (List Biological Labs, Campbell, CA, USA) was administered on the day of induction and 48 h later in EAE immunized mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…200 ng of pertussis toxin (List Biological Lab) was given ip on days 0 and 2 post immunization (pi). Mice were scored daily for appearance of clinical signs of EAE by a scale from 0 to 5 as described previously [18]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… Mice (48 weeks old) were immunized with 200 µg of myelin oligodendrocyte glycoprotein peptide (3555) (MOG) emulsified with Complete Freuds Adjuvant… Pertussis toxin (PTX), 250 ng (List Biologicals Laboratories, Campbell, CA), was injected intravenously on day 0 and again on day 2…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peptide synthesis and EAE induction:

…Mice were injected s.c. in their anks with 0.1 ml of the peptide emulsion (for a total of 100 mg MOG3555 and 400 mg M. tuberculosis/mouse) and, on the same day and 48 h later, were injected i.v. with 0.2 ml containing 200 ng Bordetella pertussis toxin (List Laboratories) dissolved in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Forty nanolitres of 1% low-salt cholera toxin B subunit (CTb) (, USA) was injected over a period of 1 min, and the needle was left in place for 10 min prior to removal, to allow for diffusion and to reduce spread up the injection tract. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunohistochemistry:

In a subset of SH animals treated with intrathecal BDNF or aCSF (n=6 each), an intrapleural injection of cholera toxin subunit B (CTB; List Biological Laboratories, Campbell, CA) was used to retrogradely label phrenic motoneurons at 3 days before the terminal experiment, as previously reported… Spinal cord sections were blocked with 10% Donkey Serum in 0.3% Triton tris-buffered saline (TBS), followed by overnight incubation with CTB antibody (goat polyclonal, #703; List Biological Laboratories) and…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

For the detection of CTb, free-floating sections were incubated for 1824 h with goat anti-CTb IgG primary antibody (List Biological Laboratories, Inc.), diluted 1:9000, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

Female SJL/J mice were purchased from Janvier (Janvier SAS, Le Genest-St-Isle, France). For active EAE, 22 mice (12 weeks old) were immunized subcutaneously with 250 µg PLP (Pepceuticals Ltd., UK) and 800 µg mycobacterium tuberculosis H37Ra (Difco) in 200 µl emulsion containing equal volumes of phosphate/buffered saline (PBS) and complete Freunds adjuvant (BD-Difco), as previously described [22] 200 ng pertussis toxin (List Biological Laboratories, US) were administered intraperitoneally at days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Macrophage Isolation and Treatment:

Wildtype and SR-A deficient mice bred on a C57/BL6 background…After 24 hrs of ENP exposure, media was aspirated, cells were washed, and 2 ml fresh media with or without 10 ng/ml LPS (Escherichia coli O111:B4, LIST Biological Laboratories Inc.) was added.

• Product #201 – LPS from Escherichia coli O111:B4