Bacterial Toxin Research Citations

Chemicals and reagents:

Lipopolysaccharide (LPS; from Escherichia coli 0111:B4) was obtained from List Biological Laboratories, Inc.

Cell culture and treatments:

The human lung adenocarcinoma epithelial cell line A549…The cells were treated with or without LPS (1 g/ml) and cytokine mixture (TNF-, IFN-, and IL-1, 20 ng/ml each) for up to 24 h before harvest of the conditioned media and cells for nitrite measurement and Western blotting…

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…Each mouse was immunized with subcutaneous injection of 100 µl MOG 3555 /CFA emulsion (containing 200 µg MOG 3555 ) and intraperitoneally injection with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) diluted in sterile PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Freunds adjuvant (both DIFCO; BD GmbH, Heidelberg, Germany). Pertussis toxin (200 ng; List Biological Laboratories, Epsom, UK) was injected on days 0 and 2 of immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

… Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was added to cells (100 ng/mL) for 16-20 h before addition of agonists.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization strategies:

For generation of Th9 cells under autoimmune conditions, SJL/j mice were immunized s.c. in the hind anks with 100 mg proteolipid protein (PLP)139151 in 100 ml CFA, as previously described (21). On days 0 and 2, mice received 300 ng pertussis toxin (List Biological Laboratories) in 250 ml endotoxin-free PBS…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Exacerbation of EAE:

…A total of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) was injected i.p. on days 0 and 2. The mice were monitored daily for clinical signs of EAE.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Simultaneously with rMOG as well as 48 h later, each mouse received intraperitoneally 200 µL Pertussis Toxin (PTX) (1 µg/mL in PBS) (List Biological Laboratories, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and stimulation of T cells with MOG peptide:

EAE was induced as described previously…Mice received 200 ng Pertussis toxin (List Biological Laboratories, Campbell, CA) intraperitoneally at the time of immunization and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For injection of bulbospinal neurons the T2 spinal cord was exposed following a midline incision, the dura was removed and three to six 200-nL injections of cholera toxin B subunit (CTB; List Biological Laboratories, USA; 1%) were made bilaterally at 0.75 mm lateral to the …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Adjuvants:

… Lyophilised CT (List Biological Laboratories, Sapphire Bioscience Pty Ltd, Redfern, Australia) was resuspended in PBS and stored at 4°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae