Citations

Sensitization of mice:

Mice were sensitized by intragastric administration of 1 mg ovalbumin (OVA, grade V, Sigma Chemicals, St. Louis, MO) or ovomucoid (OM, Sigma) plus 10 µg cholera toxin (List Biologicals, Campbell, CA, USA)as adjuvant weekly for six weeks.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Inflammation experiments:

Three-month-old mice were killed (n = 3, each group) and bone marrow-derived dendritic cells (BMDC), bone marrow-derived macrophages (BMDM), and thioglycolate-elicited peritoneal macrophages were prepared and stimulated as previously described (2832). … Cells were preincubated for 1 h with dexamethasone (Sigma-Aldrich) before stimulation with 10 ng/ml Salmonella minnesota Ultra Pure LPS (List Biological Laboratories, Campbell, CA), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Flavivirus studies:

YFV-17D was grown on Vero cells, and for antigenicity studies purified virus was exposed to dilutions of H₂O₂ (Fisher Scientific), formaldehyde (Fisher Scientific) or BPL (Sigma-Aldrich) at 2024 C, diluted to 1 g ml¹ and used to coat ELISA plates. YFV-immune mice were infected intraperitoneally with 1 10⁶ PFU of YFV-17D, and immune serum was obtained at 220 d after infection. WNV-NY99 was grown on Vero cells and purified by ultracentrifugation using 36% (w/v) sucrose cushions in PBS (pH 7.4) at 110,000g for 2.5 h at 4 C. Pellets were resuspended in PBS, pH 7.4 and inactivated with 3% H₂O₂ for 2 h at 2024 C, with inactivation confirmed by plaque assay on Vero cells before final formulation and immunization. Mice were immunized subcutaneously with 10-g doses of H₂O₂-WNV formulated with 50 g of aluminum hydroxide (alum, Sigma-Aldrich) and 5 g of monophosphoryl lipid A (MPL, List Biological Laboratories, Campbell, CA) …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

Reagents and Antibodies :

… LPS from Salmonella minnesota R595 was obtained from List Biological Laboratories. …

Results – Sp-D Binding to SIRP Is Calcium-dependent, Carbohydrate-specific, and Inhibited by LPS :

… Sp-D is also known to bind with high affinity to rough LPS, the major component of Gram-negative cell walls (19). Therefore, we assessed whether Sp-D binding to SIRP was affected by LPS from S. minnesota Re mutant that expresses a truncated rough LPS known to be a strong ligand for Sp-D (37). The Re mutant expresses the shortest form of LPS among different S. minnesota strains containing only lipid A and 3-deoxy-d-manno-octulosonic acid (38). As shown in Fig. 3C, increasing concentrations of LPS significantly decreased Sp-D binding to SIRP. We observed 50% inhibition of Sp-D binding to SIRP at a concentration of 40 g/ml LPS, which is consistent with previous reports (39).

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Stimulation assays:

Synovial fibroblasts were plated in 12-well plates (5 10⁴ cells/well) in 1 ml supplemented Dulbecco’s modified Eagle’s medium and stimulated for 2, 8, or 24 hours with 10 ng/ml tumor necrosis factor (TNF), 1 ng/ml interleukin-1 (IL-1) (both from R&D Systems), 300 ng/ml bacterial lipopeptide palmitoyl-3-cysteine-serine-lysine-4, 10 g/ml poly(I-C) (both from InvivoGen), or 10 ng/ml lipopolysaccharide (LPS; from Escherichia coli J5) (List Biological Laboratories). …

• Product #301 – LPS from Escherichia coli J5 (Rc)

Media and reagents:

… Ultrapure LPS was from List Biological Laboratories. …

Western blot:

For detection of inammasome components, HMDMs were stimulated with 10 ng/ml LPS for 6 h, and 1 mM ATP was added for the last 1 h of stimulation. For detection of IL-1b, HMDMs were stimulated with LPS for 18 h, and ATP was added during the last 3 h of LPS stimulation. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… LPS was obtained from List Biological Labs. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Blood samples were treated with either LPS (E. coli O111:B4; List Biological Laboratories, Inc., Campbell, CA, USA) or vehicle. …

• Product #201 – LPS from Escherichia coli O111:B4

Treatment of Mice with LPS and MALP2:

MALP2 and LPS (LPS from Escherichia coli O111:B4) were purchased from Alexis Biochemicals (Enzo Life Sciences, Inc., Plymouth Meeting, PA) and List Biologicals (Burlington, Ontario, Canada), respectively. MALP2 (5 g) was dissolved in 200 L of saline and intraperitoneally injected into wild-type and TLR2-/- mice. For the corresponding LPS experiment, 200 L of saline containing 0.5 mg of LPS/kg mouse were administered in wild-type and TLR4-/- mice. Control mice received 200 L of saline ip.

• Product #201 – LPS from Escherichia coli O111:B4

Induction and Scoring of EAE:

Experimental autoimmune encephalomyelitis was induced as in our prior studies (Quinn et al., 2011). … Control mice were injected with an equal volume of phosphate buffered saline (PBS) and CFA. EAE and control mice received 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 ml PBS by i.p. injection on the day of immunization (day 0) and again on day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)