Citations

Production and purification of BoNT/A and TeNT:

TeNT was purchased from List Biological (CA). …

Entry of HCR/T and HCR/A into primary cortical neurons:

Binding and entry into cortical neurons were monitored by total internal reflection fluorescence (TIRF) microscopy. … Toxin entry was measured by analyzing the epitope-tagged receptor binding domains of BoNT/A (HCR/A) and TeNT (HCR/T), which are nontoxic but retain the structure-function properties and compete with CNTs for entry into cells (9, 10, 33). …

• Product #190 – Tetanus Toxin from Clostridium tetani

… The B. anthracis proteins, protective antigen (PA), edema factor (EF), and lethal factor (LF) were purchased from List Biological Laboratories, Inc. and required HRP labeling prior to analysis. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… Recombinant Bacillus anthracis lethal factor (LF) and Clostridium botulinum neurotoxin light chain A (BoNTALC) were obtained from List Laboratories (Campbell, CA; 169A and 610A, respectively). …

Protease Inhibition Assay:

Protease inhibition assays were performed as described
previously (25), but with modifications. Biotinylated green
fluorescent protein (GFP) protease substrates for LF, BoN-
TALC, and a protease-resistant substrate (pinpointGFP)
were prepared and loaded on streptavidin microspheres as
described previously (14,25,26). Additions to wells were in
sequence as follows: (1) 4 lL protease buffer; (2) 2 lLofa
mixture of 1.5 lM LF and 5 nM BoNTALC in protease
buffer; …

• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… 1 nM cholera toxin (List Biological Laboratories, Campbell …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Protocol for tissue fixation and histological processing:

The ferrets were transcardially perfused using a 0.9% saline solution.  … 

The histology was performed on free floating sections kept in small wells; all buffer solutions were made using 0.1M PBS PH (7.4). … The sections were then incubated for a short time in 0.1M glycine solution in dH20), rinsed in PBS, then incubated overnight at 4oC in a blocking solution containing 4% normal rabbit serum (NRS), 2.5% bovine serum albumin (BSA), and a 1% Triton-X solution to reduce nonspecific staining. Then they were rinsed in PBS, then incubated for 48 hours in a solution containing 1: 5,000 dilution of goat anti-choleraganoid (primary antibody, List Biological Labs), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Next, the animals received a unilateral intraocular injection of cholera toxin subunit b (CTb; List Biological Laboratories, Inc., Campbell, CA, USA). … Primary antibodies, Goat anti-CTb, List Biological Labs, Campbell, CA, USA, 703, 7032A, 1:9000. …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Anatomical Analysis:

… For visualization of CTB-labeled sensory axons, endogenous peroxidase activity was blocked with 0.6% hydrogen peroxide and non-specific antibody reactions were blocked with 5% horse serum for 1 hr at room temperature. Sections were incubated for 72 hours at 4C with the primary CTB antibody (goat polyclonal 1:80,000 dilution, List Biological Labs) followed by incubation …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Stimulation experiments:

… Cells were stimulated with the following agents: … 100 ng/ml lipopolysaccharide (LPS) from Esherichia coli (List Biological Laboratories), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… The induction protocol included administration of 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) intraperitoneally on days 0 and 2 and emulsions of 400 mg of MOG 35-55 peptide (myelin oligodendrocyte glycoprotein, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Emulsions were prepared by mechanical Omni Mixer homogenizer and injected subcutaneously at four sites (i.e. in each flank). Pertussis toxin (300 ng/mouse; List Biological Labs, Inc., Campbell, CA, USA) in PBS was injected intraperitoneally at the time of immunization and at 72 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)