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Anthrax PA 63 – FITC Conjugate
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Products are for research purposes only and are
not for use in humans or as diagnostic agents.
View our other Bacterial Toxins Products
$480.00
#175 (50 ug)
Purity
>95%
Form
lyophilized powder
Storage temp
2-8°C
TRIM21 promotes astrocyte-mediated neuroinflammation in experimental autoimmune encephalomyelitis by stabilizing RGMa via K33-linked ubiquitination
Zhang, Wang, Cheng et al
J Neuroinflammation (2026)
Zhang, Wang, Cheng et al
J Neuroinflammation (2026)
Cited by 1
Snippet: … subsequently received an intraperitoneal injection of 200 ng pertussis toxin (PTX, 181, List Biological Labs, CA, USA) on days 0 and 2 post-immunization (total of 400 ng PTX per mouse) …
Multi-Compartmental Analysis of the Murine Pulmonary Immune Response by Spectral Flow Cytometry
Chang, Brune, Black et al
Am J Physiol Lung Cell Mol Physiol (2023) 325 (4), L518-L535 DOI: 10.1152/ajplung.00317.2022
Chang, Brune, Black et al
Am J Physiol Lung Cell Mol Physiol (2023) 325 (4), L518-L535 DOI: 10.1152/ajplung.00317.2022
Cited by 14
Snippet:
Lipopolysaccharide, Bleomycin, House Dust Mite Administration
Lipopolysaccharide (LPS, Escherichia coli O111: B4, List Biological Laboratories, Campbell, CA, USA) was administered by oropharyngeal aspiration under isoflurane anesthesia at a dose of 2.0 mg/kg of mouse body weight in 50μL of PBS. ...
A regulatory circuit controlled by extranuclear and nuclear retinoic acid receptor α determines T cell activation and function
Larange, Takazawa, Kakugawa et al
Immunity (2023) 56 (9), 2054-2069.e10 DOI: 10.1016/j.immuni.2023.07.017
Larange, Takazawa, Kakugawa et al
Immunity (2023) 56 (9), 2054-2069.e10 DOI: 10.1016/j.immuni.2023.07.017
Cited by 24
Snippet:
... Pertussis toxin List Biological Laboratories Inc 180 ...
Mediobasal hypothalamic neurons contribute to the control of brown adipose tissue sympathetic nerve activity and cutaneous vasoconstriction
Mota, Madden
J Therm Biol (2023) 114, 103551 DOI: 10.1016/j.jtherbio.2023.103551
Mota, Madden
J Therm Biol (2023) 114, 103551 DOI: 10.1016/j.jtherbio.2023.103551
Cited by 6
Snippet:
... immunohistochemistry. Briefly, sections ... were processed and incubated overnight in a goat anti-CTb antibody (List Biological Lab, #7032A6) at 1:10,000, followed by rinses in PBS and incubation ...
Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate
Weiss, Spahn, Chiang et al
Nat Chem Biol (2021) 17 (6), 684-692 DOI: 10.1038/s41589-021-00776-9
Weiss, Spahn, Chiang et al
Nat Chem Biol (2021) 17 (6), 684-692 DOI: 10.1038/s41589-021-00776-9
Cited by 31
Snippet:
... For the resistance screen, ... diphtheria toxin (List Labs) (Supplementary Information) ...
Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release
Szot, Saha, Zhang et al
J. Clin. Invest. (2018)
Szot, Saha, Zhang et al
J. Clin. Invest. (2018)
Snippet:
Flow cytometry:
Cells were trypsinized, rinsed in cold PBS-BSA, and labeled with m825-MMAE, an isotype-matched human IgG, or FITC-conjugated protective antigen (List Biologicals Laboratories) in PBS-BSA at 4o C. ...
Febuxostat ameliorates secondary progressive experimental autoimmune encephalomyelitis by restoring mitochondrial energy production in a GOT2-dependent manner
Honorat, Nakatsuji, Shimizu et al
PLoS ONE (2017) 12 (11), e0187215 DOI: 10.1371/journal.pone.0187215
Honorat, Nakatsuji, Shimizu et al
PLoS ONE (2017) 12 (11), e0187215 DOI: 10.1371/journal.pone.0187215
Snippet:
EAE induction, treatment and DNA microarray:
... EAE was induced in 8 weeks old female non-obese diabetic (NOD)/ShiJcl mice (CLEA Japan, Inc.). EAE was induced using a modified method we previously reported [11]. In brief, NOD mice were subcutaneously immunized into both flanks with 100 µg MOG 3555 (myelin oligodendrocyte glycoprotein, MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (200 l) containing Mycobacterium tuberculosis H37Ra (200 µg, Difco Laboratories), followed by intraperitoneal injections of 200 ng pertussis toxin (List Laboratories) on days 1 and 2. ...
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
Treatment with retinoid X receptor agonist IRX4204 ameliorates experimental autoimmune encephalomyelitis
Chandraratna, Noelle, Nowak
Am J Transl Res (2016) 8 (2), 1016-26
Chandraratna, Noelle, Nowak
Am J Transl Res (2016) 8 (2), 1016-26
Cited by 1
Snippet:
EAE immunization and clinical evaluation:
For active EAE, mice were immunized subcutaneously with 175 µg MOG35-55 (Anaspec) emulsified in Incompete Freunds Adjuvant (Sigma-Aldrich) supplemented with 0.4 mg Mycobacterium tuberculosis H37 (Difco Laboratories) on day 0 and given 200 ng pertussis toxin (List Biologicals) intraperitoneally (IP) on day 0 and 2. ...
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
Single-cell characterization of autotransporter-mediated Escherichia coli surface display of disulfide bond-containing proteins
Ramesh, Sendra, Cirino et al
J. Biol. Chem. (2012) 287 (46), 38580-9 DOI: 10.1074/jbc.M112.388199
Ramesh, Sendra, Cirino et al
J. Biol. Chem. (2012) 287 (46), 38580-9 DOI: 10.1074/jbc.M112.388199
Snippet:
Expression and Labeling of M18 scFv :
A standard 3-ml culture of cells harboring plasmids to surface display M18 scFv (e.g. pBAD_M18_138) was grown in LB medium (BD Diagnostics) in the presence of 30 g/ml chloramphenicol (Thermo Fisher Scientific) to an optical density (A600) of 0.6 at 25 C. Cells were then induced via the addition of 0.2% l-arabinose (Sigma) to express M18 scFv for 12 h at 25 C. The presence of M18 scFv on the surface of E. coli was characterized by the ability of intact cells to bind antigens (PA63 and PAD4) using flow cytometry. PAD4 containing FLAG epitope tag (rPAD4) was recombinantly expressed in E. coli and purified as described previously (35), and PA63-FITC was obtained from List Biological Laboratories, Inc. ...
Anthrax lethal toxin has direct and potent inhibitory effects on B cell proliferation and immunoglobulin production
Fang, Xu, Chen et al
J Immunol (2006) 176 (10), 6155-61 DOI: 10.4049/jimmunol.176.10.6155
Fang, Xu, Chen et al
J Immunol (2006) 176 (10), 6155-61 DOI: 10.4049/jimmunol.176.10.6155
Cited by 79
Snippet:
Reagents and antibodies:
Recombinant anthrax PA and LF were purchased commercially and were stored in 1:1 glycerol-water at 20C (List Biological Laboratories) for in vitro studies. Unless otherwise indicated, anthrax LT was administered in excess at concentrations of 2.5 g/ml PA and 1 g/ml LF. In selected experiments a proteolytically inactive mutant of LF was used as a negative control (E687C substitution in zinc binding site that eliminates enzymatic activity; List Biological Laboratories). ...
Anthrax PA binding assays:
Purified murine or human B cells were cultured at 4C for 30 min with FITC-labeled anthrax PA (50 g/ml; List Biological Laboratories) in the presence or absence of unlabeled anthrax PA (150 g/ml) to confirm specific binding. Stained cells were then washed with PBS and analyzed by flow cytometry (see below). Unstained cells were analyzed in parallel to establish background levels of autofluorescence.
ELISA:
Primary B cells were cultured in complete RPMI for 4 to 5 h, washed with RPMI 1640, and then stimulated as indicated in the presence or the absence of anthrax LT for 7 days. ...
Murine in vivo studies:
Mice were treated with varying doses of anthrax LT as indicated, using a fixed ratio of LF/PA of 1:2.5. LF and PA were resuspended in PBS and injected i.p. into mice in a total volume of 1.0 ml of PBS. As a negative control, selected mice were treated with PBS alone. Mice were sacrificed 3 h after treatment, and spleens were harvested for primary B cell isolation as previously described. Primary B cells were then evaluated for proliferation and IgM production.
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
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