Citations

Brainstem histology:

Mice were initially anesthetized with isoflurane and then given an intraperitoneal urethane injection … The sections were incubated overnight with primary antibodies against CT- (polyclonal goat antiserum to purified CT-, choleragenoid isolated from Vibrio cholerae type Inaba 569B) (product no. 703, lot no. 7032A5, diluted 1:10,000; List Biological Laboratories, Campbell, CA). … Western blot (manufacturer’s technical information). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Stimulation of human cells by Mtb:

Human THP-1 and U137 cell lines and ex-vivo peripheral blood mononuclear cellss were plated in RPMI 1640, HeLa cells were cultured in DMEM and A549 cells were cultured in F12K, all containing 10% FCS, 100Uml¹ penicillin, 100gml¹ streptomycin, and 2mM L-glutamine. 5 10⁵ cells were plated in 24-well plates overnight and stimulated with either Mtb, LPS, PAM2 or PAM3 (Mtb is whole-cell lysate of Mtb H37Rv (Colorado State University); LPS from Salmonella Minnesota (List Biological Laboratories, Campbell, CA, USA); …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Analysis of parasite replication in macrophages:

Mutants were rst screened in 96-well plates containing bone marrowderived macrophages. Equal volumes of parasites were transferred from 96-well plates containing HFF cells to 96-well plates containing macrophages. Parasites were allowed to infect macrophages for 4 h and then plates were rinsed with D10 media to remove extracellular parasites. D10 media supplemented with 100 ng/ml LPS (Escherichia coli; List Biologicals, Campbell, CA), and …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Experimental Autoimmune  Encephalomyelitis (EAE) Induction:
 
MOG-dependent EAE was induced in 6- to 8-week old female C57BL/6 mice via
a single peptide dose.  MOG35-55 (Bio-Synthesis, Inc. Lewisville, TX) peptide was
emulsified in complete Freunds adjuvant (CFA) with 4 mg/mL of killed Mycobacterium
tuberculosis, which was subcutaneously (s.c.) injected in 100 µL into the flank of
anesthetized mice on Day 0.  Mice also received 200 ng of pertussis toxin (PT; List
Biological Laboratories, Inc., Campbell, CA) intraperitoneally (i.p.) on Day 0 and Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were also injected i.p. with 400 ng Bordetella pertussis toxin (List Biological Laboratories) at the time of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

SJL Mice Immunization:

… At day 7, immunization boost was performed by delivering another identical subcutaneous injection of emulsion on the second shoulder. Mice were also injected intraperitoneally with 100 mL of a pertussis toxin solution (500 ng/100 mL; List Biological Laboratories, Campbell, CA) at days 0, 1, 7, and 8.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… We attempted to induce breach of the BBB by injection of complete Freund’s adjuvant (CFA, Sigma, Gibco Laboratories and Difco, containing heat-killed H37Ra Mycobacterium tuberculosis [MTB]) and pertussis toxin (PTx, List Biological Laboratories Inc) before passive transfer …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive transfer of 2D2 T cells and induction of EAE:

… For induction of EAE in RAG-1^/ mice, mice of 8-12 wks of age first received purified 2D2 T cells (1 10⁶/mouse) i.v. Twenty four hours later, 2D2 T-cell recipients were immunized s.c. with 200 jig MOG 35-55 in CFA (containing 400 jig of Mycobacterium tuberculosis) in a total volume of 100 mL. Mice received 100 ng of pertussis toxin (List Biological, Campbell, California, USA) in 200 L PBS in the tail vein immediately after the immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture:

N18TG2 neuroblastoma cells (passage numbers 2550) were maintained at 37°C under a 5% CO₂ atmosphere in complete media comprising: Dulbecco’s modified Eagle’s medium … Where indicated, N18TG2 cells were pretreated with receptor antagonists or other inhibitors prior to addition of CB₁ receptor agonists. Pertussis toxin (100 ngmL¹; List Biological Laboratories, Campbell, CA, USA) was added to cells 16-20 h before addition of agonists.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK), purchased from Genemed Synthesis (San Antonio, TX), was used as the immunogen. Mice of 8-12 wk of age were immunized s.c. with 200 mg MOG peptide in CFA (containing 400 mg Mycobacterium tuberculosis) in a total volume of 100 ml. Mice also received 150 ng pertussis toxin (List Biological, Campbell, CA) in 200 ml PBS via the tail vein immediately after the immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)