Bacterial Toxin Research Citations

Induction and Assessment of EAE:

For the induction of EAE, mice were immunized subcutaneously in the four flanks with 100 µg of MOG(3555) emulsified 1:1 in complete Freund’s adjuvant (Difco) supplemented with 500 µg of Mycobacterium tuberculosis H37Ra on day 0. In addition, 120 ng of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was injected intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Treatment of animals:

… For active induction of EAE, C57BL/6 female mice were immunized with MOG_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) of rat origin, by a modification of the method previously described … Mice were also injected i.p. with 200ng pertussis toxin (List Laboratories, Campbell CA, USA) in PBS on days 0 and 2 following the first immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Additionally, on the day of immunization and 2 days after (day 0 and 2), 100µl of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in PBS was injected ip to the mice (Miller and Karpus 2007). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… These assays were conducted using 60.8 ng/ml of PA (List Biological Laboratories, Campbell, CA) and 48.6 ng/ml of LF (List Biological Laboratories) to achieve more than 99% cell lysis at a fixed cell density of 3 10 4 cells/well as determined by toxin potency testing. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction of experimental autoimmune encephalomyelitis:

Mice were immunized with 200 µg of MOG3555 emulsified in CFA containing 4 mg/ml Mycobacterium tuberculosis (Difco) subcutaneously and given 200 ng of pertussis toxin (List Biological Laboratories) intravenously on the day of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS (lipopolysaccharide) was from List Biological …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials & Methods – Reagents:

… LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

… The Anthrax Lethal Factor and Anthrax Protective Antigen were obtained from List Biological Laboratories, INC.

Assay for the screening of the small molecule library against APA and ALF:

An analyzing solution consisting of 10 L of the Small molecule-carrying MRnS
and 2,000 L DI water was prepared. Samples containing different concentrations of free
toxin (APA or ALF, 2 pM to 20 nM, in 1X PBS buffer) were prepared and 2 L of each
sample was added to 200 L of the MRnS analyzing solution (Small molecule-MRnS). A
negative control sample was prepared in the same fashion, adding 2 L fresh 1X PBS
buffer instead of toxin (0 M APA or ALF control sample). Magnetic relaxation
measurements were performed every 15 minutes of incubation at room temperature for 1
hour. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Antigens:

…Tetanus toxoid was obtained from List Biological Laboratories (Campbell, CA).

• Product #191 – Tetanus Toxoid from Clostridium tetani

HT29 and Vero cell cytotoxicity assays:

…The plates were incubated for 48 h (37C; 5% CO2) to let monolayers form before addition of C. difficile culture supernatants…. Cell supernatants from isogenic toxin A B mutants were used as negative controls (20). Pure toxin A and toxin B (List Biological Laboratories) were used as positive controls, so that the detection limit of each assay could be determined in terms of the toxin concentration. The detection limit of the HT29 cell cytotoxicity assay was 100 pg/ml toxin A, and the detection limit of the Vero cell cytotoxicity assay was 25 pg/ml toxin B. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile