Bacterial Toxin Research Citations

EAE induction and clinical evaluation:

EAE was induced by s.c. immunization with 100 mg MOG3555 peptide in 100 ml CFA containing 0.4 mg Mycobacterium tuberculosis H37RA (Difco); 200 ng pertussis toxin (List Biological Laboratories) was administered i.v. on days 0 and 2. Clinical scoring was: 1 = limp tail; 2 = hind limb paresis or partial paralysis; 3 = total hind limb paralysis; 4 = hind limb paralysis and body or front limb paresis or paralysis; 5 = moribund.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

Experimental autoimmune encephalomyelitis was induced in 4-mo-old male B6 or B6.Sle1c2 mice. On day 0, mice received an emulsion of 50 mg myelin oligodendrocyte glycoprotein peptide sequence 3555 and 500 mg Mycobacterium tuberculosis (Difco) in IFA (Sigma-Aldrich) via s.c. injections at the base of the tail. In addition, 500 ng pertussis toxin (List Biologicals) was administered i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Material:

… Pertussis Toxin (Catalog #179A; List Biologicals, Campbell, CA). …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (salt-free; List Biological Laboratories, Campbell, CA). It is sold as a lyophilized powder. Reconstitute with PBS to a final concentration of 2 g/ml and store at 4 C. 10. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction and Assessment of EAE:

For the induction of EAE, mice were immunized subcutaneously in the four flanks with 100 µg of MOG(3555) emulsified 1:1 in complete Freund’s adjuvant (Difco) supplemented with 500 µg of Mycobacterium tuberculosis H37Ra on day 0. In addition, 120 ng of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was injected intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Treatment of animals:

… For active induction of EAE, C57BL/6 female mice were immunized with MOG_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) of rat origin, by a modification of the method previously described … Mice were also injected i.p. with 200ng pertussis toxin (List Laboratories, Campbell CA, USA) in PBS on days 0 and 2 following the first immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Additionally, on the day of immunization and 2 days after (day 0 and 2), 100µl of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in PBS was injected ip to the mice (Miller and Karpus 2007). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… These assays were conducted using 60.8 ng/ml of PA (List Biological Laboratories, Campbell, CA) and 48.6 ng/ml of LF (List Biological Laboratories) to achieve more than 99% cell lysis at a fixed cell density of 3 10 4 cells/well as determined by toxin potency testing. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction of experimental autoimmune encephalomyelitis:

Mice were immunized with 200 µg of MOG3555 emulsified in CFA containing 4 mg/ml Mycobacterium tuberculosis (Difco) subcutaneously and given 200 ng of pertussis toxin (List Biological Laboratories) intravenously on the day of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS (lipopolysaccharide) was from List Biological …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/