Bacterial Toxin Research Citations

Degranulation assay:

Mice were immunized with 10 mg MOG 3555 peptide emulsied in CFA (Difco) supplemented with 4 mg/ml Mycobacterium tuberculosis (H37RA; Difco). Each mouse also received 200 ng pertussis toxin (List Biological Laboratories) intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Mice were immunized with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freunds Adjuvant (CFA; Difco Laboratories, Detroit, MI) subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (PTX; List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… injections of two different tracers into subdivisions of the mesopallium, … and cholera toxin B subunit (CTB, choleragenoid; List Biological Laboratories, Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

… Pertussigen (PTx)(List Biological Laboratories, Campbell CA) was administered intraperitoneally on day 0 (75 ng) and day + 2 (200 ng) with respect to the day of T cell transfer. EAE scores were determined …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Purified recombinant protective antigen (rPA) was obtained from three different sources; 1) Dr. Stephen H. Leppla, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD; 2) List Biologicals, Campbell, CA or …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Mice:

C57BL/6 (CD45.2⁺) mice and congenic C57BL/6 (CD45.1⁺) mice were bred in and provided by the animal facility of the Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany. … Cell ablation was achieved by a single i.p. injection of DT (30 ng/g body mass) (List Biological Laboratories) unless stated otherwise. Note that, after birth, mice heterozygous or homozygous for the Ccx-Ckr1-eGFP knock-in allele have no overt thymus phenotype (7).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental autoimmune encephalomyelitis (EAE) model:

For analysis of in vivo-generated Th17 cells and Treg cells from an inflammatory site, active EAE was induced and CNS infiltrating leukocytes were obtained as described [32]. Briefly, C57BL/6 mice were immunized by subcutaneous injection in the lower back with 200 µg myelin oligodendrocyte glycoprotein (MOG)3555 (MEVGWYRSPFSRVVHLYRNGK) peptide emulsified at a 11 ratio with complete Freund’s adjuvant containing 150 µg Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). Pertussis toxin (List Biological Laboratories, Campbell CA) was administered on day 0 (200 ng) and day +2 (200 ng) with respect to the immunization day. Only symptomatic mice were used at 14 days after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and Reagents:

Wild-type C57BL/6 mice were purchased from Jackson Labs. … Control mice received PBS. In vitro stimulation of single-cell suspensions from the lamina propria was performed with a concentration of 200 ng/ml of flagellin. Diphtheria toxin (List Biological Laboratories) was administered every other day at a dose of 10 ng/g starting 6 days prior to injection of flagellin. Mice received a total of three doses.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice and Reagents:

Wild-type C57BL/6 mice were purchased from Jackson Labs. … Control mice received PBS. In vitro stimulation of single-cell suspensions from the lamina propria was performed using a concentration of 200 ng/mL of flagellin. Diphtheria toxin (List Biological Laboratories) was administered every other day at a dose of 10 ng/g starting 6 days prior to injection of flagellin. Mice received a total of 3 doses.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

… The PA ligand was purchased from List Laboratories (Campbell, USA). …

Surface modification:

… After washing with HBS for 2 min at a flow rate of 30 m/min, 100 g/ml of PA was introduced and incubated for 30 min with a slow flow rate of 3 l/min for covalent binding to the SAM layer. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis