Bacterial Toxin Research Citations

Molecular weight markers (MW) are indicated to the left of the image. The arrow points to the migration of the standard (STD) loaded at 100 ng (TcdB, List Biologicals).

• Product #155A – Toxin B from Clostridium difficile

… BoNT/A was purchased from List Biological Laboratories (Campbell, CA, USA). …

High K⁺-evoked overflow:

… Synaptosomes were then stimulated with KCl (25 mmol/L or 65 mmol/L). The high K⁺-solutions were prepared by substituting NaCl for equal amounts of KCl to maintain isotonicity. Eight hundred l samples of superfusate were collected at 1, 2, 5 or 10 minutes of superfusion in ice-cold Eppendorf tubes. In some experiments synaptosomes were incubated with 300 nmol/L BoNT/A for 2 hours at 37°C and then high K⁺ overflow experiments were performed. …

Sensitization and Challenge of Mice

Five-week-old C3H/HeJ mice were sensitized orally with 1 mg of native β-LG in 0.2 M bicarbonate buffer containing 20 μg of cholera toxin (List Biologicals, Campbell, CA) on d 0, 7, 14, 21, 28, and 35 by gavage. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… (12-18 per group) were immunized with 50 g of the E. coli-expressed recombinant protein (ie, D1-3, D1-2, D3, or NS1) by the in or intraperitoneal (ip) route, using 20 µl volumes containing 1 µg of cholera toxin (CT; List Biological Laboratories, Campbell, CA …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mouse model of peanut allergy:

… Briefly, mice were sensitized by oral gavage on days 1, 8, and 15 with 10 mg ground, roasted peanuts plus 20 µg cholera toxin (List Biological Laboratories, Inc.) and boosted on day 22 with 50 mg ground, roasted peanuts plus 20 µg cholera toxin. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Surgery and axonal tracing:

Adult female Sprague Dawley Rats (n=12) were divided into 4 groups (n = 3/group): each had a different cell transplant of GRP predifferentiated with FBS, BMP-4, CNTF, or control bFGF for 6 days. …

Three weeks after the surgery to create dorsal column lesions was performed, BMP-4, CNTF, and bFGF transplanted animals were anesthetized with an XAK cocktail and an incision was made into the upper hindquarters to expose the sciatic nerve. A 10-uL Hamilton syringe was inserted into each sciatic nerve and 2 to 3 L of Cholera toxin B subunit (CTB) (10 g/L; List Biological Laboratories, Campbell, CA) was injected to label ascending sensory axonal projections. Animals were sacrificed 3 days after CTB injection.

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

 Cell culture:

RAW 264.7 cells were maintained in RPMI1640 supplemented with 10% FBS and 200mg/ml gentamicin (Invitrogen Life Technologies). For stimulation of the RAW 264.7 cell, cells were plated in 6-well culture plates (3 106 cells/well) overnight. The overnight growth was performed to allow resting before stimulation. Cells were then washed twice with Opti-MEM (Invitrogen Life Technologies) and stimulated with 50ng/ml E. coli O111:B4 Ultrapure LPS (List Biological Laboratories) …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

… Pertussis toxin was obtained from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… the emulsion was mixed with Freund full adjuvant (Difco Laboratories, USA) containing the death bacterium, and the Pent Balbiter was immunized with the lateral abdominal subcutaneous of the mouse. At the first immunization and after 48 hours, 50 ng whooping toxins per pet (List biological Laboratories, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Disease induction:

Active EAE was induced in mice by subcutaneous injection in the lower flank with 200µg MOG3555 peptide emulsified at a 1:1 ratio with CFA containing 200µg heatkilled Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). Pertussis toxin (PTx; List Biological Laboratories, Campbell, CA) was administered on day 0 (75ng) and day +2 (200ng) with respect to the immunization day. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)