Bacterial Toxin Research Citations

PTx (List Biological Laboratories) was dissolved in PBS (100 ng/l).

Experimental autoimmune encephalomyelitis (EAE):

… PTx facilitates T cell access to the CNS, presumably via TLR4-mediated induction of adhesion molecules in the endothelium of the blood-brain barrier84. In addition, PTx contributes to the break of T cell tolerance, promoting clonal expansion and cytokine production by T cells79,80. This immunization model promotes the activation of TH cells, specifically of the TH1 and TH17 lineages79,80. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Freund’s adjuvant (CFA, heat-killed H37Ra, Difco). Mice received pertussis toxin (PTx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). Animals were monitored daily for clinical signs …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune myocarditis:

Previously well-established methods were used to induce EAM [14]. Briefly,100 µg commercially fMOC synthesized MyHC₆₁₄₆₂₉ [15] was emulsified 1:1 in CFA (Sigma, St. Louis, MO) supplemented with 5 mg/mL of heat-killed M. tb strain H37Ra (Difco, Detroit, MI), and installed sc in mice twice on days 0 and 7. On day 0, mice also received 500 ng of pertussis toxin ip (List Biologicals, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of EAE:

SJL/J female mice (57 weeks-old) were immunized subcutaneously (s.c.) with 200 µg PLP in a 0.2 ml emulsion comprised of equal volumes of PBS and complete Freund’s adjuvant (CFA) containing killed mycobacterium tuberculosis strain H37RA (at a final concentration of 4 mg/ml, Difco, Detroit, MI). The PLP/CFA emulsion was administered to regions above the shoulder and the flanks (total 4 sites; 50 µl at each injection site). In addition, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Degranulation assay:

Mice were immunized with 10 mg MOG 3555 peptide emulsied in CFA (Difco) supplemented with 4 mg/ml Mycobacterium tuberculosis (H37RA; Difco). Each mouse also received 200 ng pertussis toxin (List Biological Laboratories) intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Mice were immunized with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freunds Adjuvant (CFA; Difco Laboratories, Detroit, MI) subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (PTX; List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… injections of two different tracers into subdivisions of the mesopallium, … and cholera toxin B subunit (CTB, choleragenoid; List Biological Laboratories, Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

… Pertussigen (PTx)(List Biological Laboratories, Campbell CA) was administered intraperitoneally on day 0 (75 ng) and day + 2 (200 ng) with respect to the day of T cell transfer. EAE scores were determined …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Purified recombinant protective antigen (rPA) was obtained from three different sources; 1) Dr. Stephen H. Leppla, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD; 2) List Biologicals, Campbell, CA or …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Mice:

C57BL/6 (CD45.2⁺) mice and congenic C57BL/6 (CD45.1⁺) mice were bred in and provided by the animal facility of the Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany. … Cell ablation was achieved by a single i.p. injection of DT (30 ng/g body mass) (List Biological Laboratories) unless stated otherwise. Note that, after birth, mice heterozygous or homozygous for the Ccx-Ckr1-eGFP knock-in allele have no overt thymus phenotype (7).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae