Citations

Bacterial Toxin Research Citations

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5030 total record number 58 records this year

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5030 citations found

Ultra Sensitive HPLC Detection Assay for Botulinum Neurotoxin Type A

Christian, T.; Suryadi, K.; Shine, N.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

Evidence for Endotoxin Contamination in Plastic Na+ – Heparin Blood Collection Tube Lots

Newhall KJ, Diemer GS, Leshinsky N, Kerkof K, Chute HT, Russell CB, Rees W, Welcher AA, Patterson SD, Means GD

Product: LPS from Escherichia coli O111:B4

  • CYTOKINE PRODUCTION ASSAY AND MEASUREMENT OF INTERLEUKIN-1 AND TNF-:

    … LPS (List Biological Laboratories, cat #201) was diluted in assay medium. Assay medium consisted of RPMI 1640 (Invitrogen Life Technologies Corporation, cat #11875) containing 1 penicillin/streptomycin/glutamine (Invitrogen, cat #10378) and 0.1% human serum albumin (Bayer AG, cat #69220). Cultures of 50% whole blood/50% assay medium in a final volume of 200 L were incubated for 18 h at 37 C and 5% CO2 in the presence of 0 or 100 g/L LPS in a 96-well tissue-culture plate (BD Biosciences, cat #353072). LPS stimulation was initiated <1 h after blood draw and samples were run as triplicates. …

In Macrophages, Caspase-1 Activation by SopE and the Type III Secretion System-1 of S. Typhimurium Can Proceed in the Absence of Flagellin

Hoffmann C, Galle M, Dilling S, Kppeli R, Mller AJ, Songhet P, Beyaert R, Hardt WD

Product: Unspecified List Labs LPS

  • Cell culture and infection experiments:

    RAW264.7 macrophage-like cells (ATCC No. TIB-71) were cultured in RPMI (10% FCS, 1% L-Glutamine) at 37C/5% CO2. For infection experiments for analysis of LDH and Il-1 release, cells were seeded in 96 well plates the day before. 20000 RAW264.7 cells were seeded per well and were pre-stimulated with 1 g/ml E. coli LPS (List Biological Laboratories, California) overnight to induce expression of pro-IL-1. …

    Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Characterization of SNARE Cleavage Products Generated by Formulated Botulinum Neurotoxin Type-A Drug Products

Hunt T, Rupp D, Shimizu G, Tam K, Weidler J, Xie J.

Product: SYNTAXtide® (o-Abz/Dnp) Peptide Substrate for C. botulinum Type C Neurotoxin

Anthrax LFn-PA Hybrid Antigens: Biochemistry, Immunogenicity, and Protection Against Lethal Ames Spore Challenge in Rabbits

Li Q, Peachman KK, Sower L, Leppla SH, Shivachandra SB, Matyas GR, Peterson JW, Alving CR, Rao M, Rao VB

Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Selective modulation of TLR4-activated inflammatory responses by altered iron homeostasis in mice

Wang L, Harrington L, Trebicka E, Shi HN, Kagan JC, Hong CC, Lin HY, Babitt JL, Cherayil BJ

Product: Unspecified List Labs LPS

  • Macrophage stimulation:

    Thioglycollate-elicited peritoneal macrophages were prepared as previously described (25, 41) and stimulated with 100 ng/ml LPS (Ultra-pure; List Biological Laboratories), …

    Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Sensitive, In Vitro, Bifunctional, Potency Assay For Botulinum Neurotoxin Type A

Christian, T.; Shine, N.

Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

  • Materials:

    SNAPtide substrate #520 contains the FRET pair o-aminobenzoic acid/2,4 dinitrophenyl (o-Abz/Dnp) and SNAPtidesubstrate Mca/Dnp contains (7methoxy-coumarin-4-yl)acetyl/2,4 dinitrophenyl as its FRET pair. Botulinum Neurotoxin Type A (Product #130A) and the SNAPtidesubstrates are products of List Biological Laboratories.

FRET Peptide Substrates for BotulinumNeurotoxins Types A, B, C, and E

Shine, N.; Endrukaite,E.; Christian, T.

Product: VAMPtide® Peptide Substrate (o-Abz/Dnp) Substrate for C. botulinum Type B Neurotoxin

Kinetics of Lethal Factor and Poly-D-Glutamic Acid Antigenemia during Inhalation Anthrax in Rhesus Macaques

Boyer AE, Quinn CP, Hoffmaster AR, Kozel TR, Saile E, Marston CK, Percival A, Plikaytis BD, Woolfitt AR, Gallegos M, Sabourin P, McWilliams LG, Pirkle JL, Barr JR

Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis

  • LF quantification:

    … To achieve the necessary dynamic range and to account for high-sensitivity rapid-response sample analysis, two standard curves were prepared, one for 20 ng/ml, l (low volume [LV]) and the other for 200 ng/ml, l (high volume [HV]) serum volumes. In addition, three quality control samples (QCs) were prepared in human serum pools (Interstate Blood Bank, Memphis, TN) using recombinant LF (List Biological Laboratory, Campbell, CA) and validated. …

    PA antigen capture immunoassay:

    Microtiter plates (Thermo Labsystems, Franklin, MA) were coated with monoclonal anti-PA antibody AVR1046 obtained by the same methodologies used for anti-LF monoclonal described previously (4) at 1.28 g/ml in 0.01 M phosphate-buffered saline (PBS), pH 7.4. Coated plates were washed with a wash buffer consisting of PBS and 0.1% Tween 20, pH 7.4. Recombinant PA (rPA; List Biological Laboratories, Inc., Campbell, CA) was serially diluted twofold using a 1/25 or a 1/100 dilution of normal rhesus macaque serum (RMS). The rPA dilutions formed a seven-point standard curve from 0.6 to 37.5 ng/ml. …

    Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).